[Education projects as an effective tool for developing meta-skills among moscow primary care providers: a case of "Scientific laboratory: Moscow polyclinic" project].

Against the background of a large-scale modernization of primary health care, introduction of new treatment and diagnostic methods and clinical guidelines, primary care physicians have an increasing need to improve professional skills, expand horizons and adapt to a rapidly changing reality, requiring the development of meta-skills, for example, critical thinking and research competencies. The purpose of the study was to assess the possibility of developing meta-skills among primary care providers through an educational project exemplified by the "Scientific Laboratory: Moscow Polyclinic" urban project.

[The clinical practice guidelines for primary hyperparathyroidism, short version].

Primary hyperparathyroidism (PHPT) is an endocrine disorder of parathyroid glands characterized by excessive secretion of parathyroid hormone (PTH) with an upper normal or elevated blood calcium level. Classical PHPT refers to a symptomatic, multi-system disorder, wich can lead to a significant decrease in the quality of life, disability of patients, and even an increased risk of premature death. Hypercalcemia and the catabolic effect of PTH on various cells are considered as the main pathogenetic mechanisms of the PHPT associated complications.

[Review of clinical practice guidelines for hypoparathyroidism].

Hypoparathyroidism is a rare disorder characterized by the absent or inappropriately decreased serum parathyroid hormone in the parathyroid glands, which is accompanied by impaired calcium-phosphorus metabolism.The main etiology of hypoparathyroidism remains damage or removal of the parathyroid glands during neck surgery. In view of the incidence of thyroid cancer, primary hyperparathyroidism and other pathologies of the neck organs, which radical treatment can lead to the parathyroid gland impairment, an increased number of patients with hypoparathyroidism is expected.

Treatment of high fracture risk patients in routine clinical practice.

Unlabelled: A retrospective cohort study determined the high incidence of recurrent fractures in osteoporotic patients with high fracture risk during the observation. The strategy of starting treatment with more potent regimens (zoledronic acid, denosumab and/or teriparatide) seems to have the best secondary fracture prevention efficacy.

Objective: This paper describes the various medical therapy regimens prescribed to osteoporotic patients with high fracture risk and the result of treatment.

[Primary hyperparathyroidism in Russia according to the registry].

Background: There are no large-scale epidemiological studies on primary hyperparathyroidism (PHPT) in Russia. The high prevalence of the disease, the high risk of disability and death in this cohort of patients requires the study of the epidemiological and clinical structure of PHPT to determine the extent of medical care.

Aim: Evaluate the frequency of PHPT detection and characterize its clinical forms in Russia using an online registry.

Polariton-assisted splitting of broadband emission spectra of strongly coupled organic dye excitons in tunable optical microcavity.

Resonance interaction between a localized electromagnetic field and excited states in molecules paves the way to control fundamental properties of a matter. In this study, we encapsulated organic molecules with relatively low unoriented dipole moments in the polymer matrix, placed them in tunable optical microcavity and realized, for the first time, controllable modification of the broad photoluminescence (PL) emission of these molecules in strong coupling regime at room temperature. Notably, while in most previous studies it was reported that the single mode dominates in the PL signal (radiation of the so-called branch of the lower polariton), here we report on the observation of two distinct PL peaks, evolution of which has been followed as the microcavity mode is detuned from the excitonic resonance.

International intercomparison of retrospective luminescence dosimetry method: sampling and distribution of the brick samples from Dolon' village, Kazakhstan.

There are still many differences in dose estimates depending on the applied methods of retrospective dosimetry in the areas, which were affected by nuclear tests at the Semipalatinsk nuclear test site (SNTS). In order to provide more correct estimation of radiation doses to population in proximity to the SNTS an International Intercomparison of Retrospective Luminescence Dosimetry (RLD) method had been proposed. It was suggested there be a comparison of the dose estimates for the brick samples from the buildings in the settlement, suffered following nuclear tests at the SNTS.

A gradient of radioactive contamination in Dolon village near the SNTS and comparison of computed dose values with instrumental estimates for the 29 August, 1949 nuclear test.

Spatial distributions of soil contamination by 137Cs (89 sampling points) and 239+240Pu (76 points) near and within Dolon village were analyzed. An essential exponential decrease of contamination was found in Dolon village: the distance of a half reduction in contamination is about 0.87-1.

Some immunological correlations between thyroid pathology and ophthalmopathy.

Hormonal and immunogenetic investigation of patients with ophthalmopathy in the presence of thyroid pathology and without it made it possible to come to a conclusion of the independence of both diseases. Immunological markers of thyroid pathology in the presence of ophthalmopathy are noted less frequently than in thyroid diseases without ophthalmopathy. The latter is characterized by the presence of specific autoantibodies to eye muscle membranes in the patients' sera.

Antigen shared by HeLa-like human cell line and gastric mucosa.

An antigen of human gastric mucosa immunologically related to the antigen of established HeLa-like cell lines (CL-GMA) is described. In gel-immunodiffusion test the antigen was revealed in 10/10 samples of normal gastric mucosa (including all parts of stomach), in 15/16 samples of cancer patients' gastric mucosa 5-10 cm distant from tumor and in 2/2 samples of ulcer patients' mucosa 5-10 cm distant from the ulcer. However, the antigen was undetectable at a distance 1-2 cm from ulcer.

Detection in the blood serum of breast cancer patients of circulating immune complexes containing antigens showing common epitopes with structural proteins of mouse mammary tumour virus (MMTV).

In the blood serum of a proportion of breast cancer patients (29.4%) immune complexes specifically reacting with the immunoglobulins of the serum against MMTV structural proteins were detected using indirect enzyme-linked immunosorbent assay (ELISA). The presence of such complexes could decrease the titre of antibodies reactive with MMTV in the blood serum of breast cancer patients.

Antibodies to MuMTV proteins in the sera of mammary carcinoma patients' healthy daughters.

The presence of antibodies to MuMTV-related proteins in sera of 31 mammary carcinoma patients' healthy daughters have been tested by indirect immunoperoxidase method (ELISA). Ten of them (32.3 +/- 8.

A new cell system for the study of mouse mammary tumour virus.

A stable cell line derived from mammary gland cancer of Djungarian hamster is proposed for the study of oncogenic properties of mouse mammary tumour virus (MMTV). The conditions of infection and expression of the viral genome in this cell line are described in addition to the chromosomal composition or the original and infected cells. The MMTV-infected cells showed increased colony formation in semi-solid media.

Hormone-dependent enhancement of neoplastic phenotype expression by MuMTV-infected heterologous cells, and MuMTV-induced cellular antigens.

A new property, i.e. acquisition of insulin (insulin + dexamethasone)-mediated enhancement of anchorage independence by heterologous MuMTV-infected cells, is described.

Dependence of the structural MuMTV protein expression on hormone-induced murine mammary carcinoma GR/mt cell differentiation in vitro.

Effect of three hormones on the clonal cell line, originating from the stable GR/mt mammary carcinoma (MC) cell line was studied. In the hormones free media the cells had fibroblast-like shapes. Normal differentiation markers and MuMTV protein processing could not be revealed.

Use of an electroimmunodiffusion method on cellulose acetate films for the detection of antigenicity in human breast cancers that is related to the major core antigen of mouse mammary tumor virus (MuMTV).

A highly sensitive electroimmunodiffusion method on cellulose acetate films, developed by Abelev on the basis of isotachoelectrophoresis, was applied to search of MuMTV-related antigenicity in tumor tissue and sera of breast cancer patients. In 2 out of 5 breast cancer extracts, protein fractions corresponding to a molecular weight of about 50,000 daltons were seen to exhibit antigenicity related to the major core antigen of MuMTV (p27). This antigenicity could not be detected in the sera of the patients.

Human mammary gland associated antigens. Lack of D-type retravirus-induced antigens.

Antisera against tumor-associated and organ-specific antigens of human mammary glands, as well as mammary carcinoma patient sera were used in cross-reactions with the test-system for a nucleoid antigen of Hep-2 retravirus. None of the antigens, revealed in mammary carcinomas and human milk seems to be either viral structural, or virus-induced cellular antigen. No cross-reactivity was revealed between preparations, containing A-type particles, isolated from Hep-2 cells and human milk and mammary carcinomas.

Immunologic study of antigens and molecular hybridization of nucleic acids from milk of breast cancer patients.

Comparative study has shown that by the gel diffusion assay both anti-HEp-2 virus serum and anti-breast cancer milk ultrasediment serum precipitated an identical antigen in human milk specimens, which was not identical with the group specific antigen of the HEp-2 virus. At the same time tha anti-breast cancer milk serum did not precipitate the HEp-2 virus. However, molecular hybridization experiments revealed nucleotide sequences homologous to nucleic acids of oncornavirus of D-type in milk obtained from breast carcinoma patients as well as in milk of healthy donors.