Immunohistochemical study of proteins linked to apoptosis in rat fetal kidney cells following prepregnancy adriamycin administration in the mother.

Adriamycin is an antibiotic of the anthracycline group. In a previous study, we showed that administration of a single dose of adriamycin (i.p.

[The role of integrins in the physiologic and pathogenic processes].

Cell adhesion molecules (CAM) are a numerous, diverse group of cell surface proteins, which are both receptors and ligands for receptors. Their functions include adhesion, recognition, cell-cell interaction, and communication between mediate cells and extracellular matrix. The following groups of CAM can be distinguished: seletins, integrins, cadherins and other isoforms, including CD 44.

Influence of dizocilpine (MK-801) on neurotoxic effect of dexamethasone: behavioral and histological studies.

Elevated levels of endogenous glucocorticoids (GCs) or prolonged treatment with high doses of dexamethasone (DEX) or other GCs preparations are frequently associated with psychosis as well as cognitive deficits, such as the impairment of memory and learning. GCs potentiate stress or ischemia-induced accumulation of excitatory amino acids in the extracellular space of hippocampus. The antagonism of glutamate receptors may potentially improve safety profile of therapy with GCs.

In-vitro mechanical impacts on calves' proximal femurs: significance of mechanical weakening of the femoral head in the etiology of Perthes disease in children.

Experimental research was conducted to determine the effects of mechanical forces on the hip joint in the etiology of Perthes disease in children. The authors aimed to identify areas of lower resistance to mechanical forces in a growing femoral head. Calves' femurs, used as experimental models, were repeatedly subjected to mechanical impacts.

The influence of a single dose of adriamycin on the pregnant rat female liver-histological and histochemical evaluation.

The purpose of the study was histological evaluation of the liver of a pregnant rat, which 4 weeks before planned pregnancy was administered adriamycin intraperitoneally in a dose of 5 mg/kg of body weight. Focal damage of hepatocytes ("naked nuclei" which were the evidence of cell damage) were observed in histological preparations. Significant steatosis and vessel damage were visible as well.

Increased apoptosis in the adult rat liver after a single dose of adriamycin administration.

The purpose of this study was the evaluation of hepatocytes in the adult rat's liver after adriamycin administration in a single dose. The presented above morphological changes, on tissue, cell and ultrastructural level are the evidence of persistent and irreversible damage of liver tissue via adriamycin. Cells damaged in that way waste away.

Histological examination of the liver after experimental administration of MK-801 and dexamethasone.

The aim of the research was histological assessment of the influence of MK-801 (NMDA receptor antagonist) and dexamethasone on the liver. The experiment was carried out on adult Albino-Swiss mouse males. MK-801 was administered in the dose of 0.

Morphological changes in adrenal cortex--the final effect of angiotensin II receptor antagonists.

The influence of losartan, angiotensin receptor on the histological image of the adrenal glomerular layer of white rats was revealed. The drug was administered in the therapeutic and ten times higher dose. It was manifested by the decrease of glandular cells and prevalence of blastic cells of the cortex.

Investigations of ultrastructure of damaged and regenerated skeletal muscle fibers.

Our investigations concerned the head of the parietal part of quadriceps femoris, and we based our investigation on observations of the ultrastructure of muscle fibers using an electron microscope. We observed tissue samples taken from patients (10 men) 25-35 years old, who had old damage of knee joint ligament (after about 6 week's immobilization). In the first group, segments of tissue of parietal head of quadriceps femoris were taken inter-operationally from patients in whom there was found old damage of knee joint ligament.

Ultrastructure of the hepatocytes after application of azithromycin (Sumamed).

Azithromycin (Sumamed, PLIVA Cracow) was administered intragastrically to rats in doses 10 times and 100 times bigger than the therapeutic dose (according to the indications for a 5-day treatment). Ultrastructural examinations of the liver were performed. Changes observed in the morphology of hepatocytes and Browicz-Kupffer cells (swelling of mitochondria, more numerous lysosomes, weaker RER content and granular material inside biliary canaliculi) may indicate temporary violation of the functional equilibrium in the liver due to the application of azithromycin.

High doses of atorvastatin (Sortis) modify the secretory process in the exocrine portion of the pancreas.

Changes were observed in the ultrastructure of the pancreatic acinar cells in rats after administration of atorvastatin (Sortis) in the therapeutic and ten times larger dose for the period of 6 weeks. It was found that atorvastatin given to rats in the therapeutic dose (80 mg/day) stimulated the secretion in pancreocytes, whereas the ten times higher dose of atorvastatin distorts the complicated process of the release of the secretion from the cell.

Histological picture of intestinal mucosa of rats after simultaneous administration of atorvastatin and ethyl alcohol.

The rats received atorvastatin in the therapeutical dose and 10x higher, 20% solution of ethanol and ethanol with atorvastatin for 6 weeks. The microscopic investigations (H + E staining, Masson's method, PAS method by McManus) showed no changes in histological picture of intestinal mucosa after giving atorvastatin. The rats receiving alcohol and alcohol with atorvastatin showed similar changes, but different than the first groups.

Histological and ultrastructural changes in cross-striation muscle cells, under the influence of atorvastatin-reductase HMG-CoA inhibitor.

The investigation was carried out on 15 Wistar rats--males weighing about 200 mg each. The animals were divided into three groups: one control group and two experimental groups, with five animals in each. In experimental group I the animals received emulsion of Atorvastatin in distilled water at the therapeutical dose of 80 mg/kg of body mass, by stomach tube for 6 weeks.

The pregnancy coexisting with experimentally induced nephrotic syndrome (NS) in rats--the histological assessment of kidneys in light microscopy.

The purpose of this study was histological evaluation in light microscopy of kidneys of pregnant female rats, which before pregnancy suffered from adriamycin-induced NS. The result show numerous changes in glomerules and renal convoluted tubules, which were the evidence of increased protein loss with urine.

Nephrotic syndrome (NS) and pregnancy in rat--evaluation of kidney in electron microscopy.

The purpose of this study was the histological evaluation of kidneys on the ultrastructural level. In the experiment there were used pregnant female rats in which gestation coexisted with adriamycin-induced NS. Results showed numerous focal changes in kidney glomerules and tubules which were the evidence of increased protein loss and filtration barrier damage as the main cause of increased permeability for proteins.

Glomerulosclerosis 4 weeks after administration of adriamycin in a single dose.

The purpose of this study was the histological assessment of kidney glomerules in light microscopy after a single dose of adriamycin. Results of lab investigation showed that as soon as 4 weeks after adriamycin administration given peritoneally appears focal, segmental glomerulosclerosis.

Ultrastructure of kidney renal proximal convoluted tubules of experimental animals after Cladribine (2-CdA) administration.

The proximal convoluted tubules of the kidney of white Wistar rats were examined. The animals were given Cladribine (2-CdA) subcutaneously at dosages of 0.07 mg/kg b.

The ultrastructure of kidney renal corpuscles of experimental animals after Cladribine (2-CdA) administration.

The renal glomeruli of the kidney of white Wistar rats were examined. The animals were given Cladribine (2-CdA) subcutaneously at dosages of 0.07 mg/kg b.

The influence of experimental administration of ethanol and cephalexin on stomach mucous barrier of rat.

The Wistar rat males weighing approximately 200 g were administered 20% ethanol ad libitum during 10 days, cephalexin in the dose of 42 mg/24 h, and simultaneously ethanol and cephalexin in the above-mentioned doses. After 10 days the animals were decapitated and stomach specimens were taken from the major curvature region for examinations. They were fixed in 10% formalin, dehydrated and immersed in paraffin.

Histomorphology of renal proximal convoluted tubules of kidney of experimental animals after Cladribine (2-CdA) administration.

The proximal convoluted tubules of the kidney of the white Wistar rats were examined. The animals were given Cladribine (2-CdA) sub-cutaneously at the dose: 0.07 mg/kg b.

Histomorphology of renal corpuscles of kidney of experimental animals after Cladribine (2-CdA) administration.

The renal corpuscles of the kidney of white Wistar rats was examined. The animals were given Cladribine (2-CdA) subcutaneously at the dosages of 0.07 mg/kg b.

Histomorphometry of nuclei of proximal convoluted tubules epithelium of kidney of experimental animals after Cladribine (2-CdA) administration.

The area of the section of nuclei of the epithelium cells lining the proximal convoluted tubules of the kidney of white Wistar rats was examined. The animals were given Cladribine (2-CdA) subcutaneously at the dosages of 0.07 mg/kg b.

Histological examinations of chief and parietal cells of the rat gastric glands after experimental administration of cephalexin and ethanol.

The experiment was carried out on Wistar rat males weighting about 200 g. Animals from experimental group I received 20% ethanol for drinking (ad libitum), animals from experimental group II--cephalexin in the dose of 42 mg/24 h, animals from experimental group III--cephalexin and ethanol in mentioned doses. After 10 days animals were decapitated and specimens of the stomach were taken from the greater curvature.

Late effects of adriamycin single dose on fetal rat kidney-ultrastructural assessment.

The purpose of the study was ultrastructural evaluation of long-lasting activity of an antibiotic from anthracycline group-adriamycin (ADR), on fetal kidneys from rat females which 4 weeks before fertilization were given a single dose of adriamycin intraperitoneally. The results showed the damage of glomerular filtration barrier (fusion of podocytes' foot processes) and degenerative lesions in tubular epithelial cells (EC). Those changes were described in literature in the case of adriamycin induced nephrotic syndrome in adult rats.

Histological and histochemical assessment of the effects of a single dose adriamycin on fetal rat kidney.

Adriamycin (ADR) induces nephritic syndrome (NS) in adult rats. Therefore, effects of ADR in a single dose of 5mg/kg body weight given intraperitoneally to the mothers at 4 weeks before pregnancy were assessed on fetal rat kidneys in the present study. It induces increased amounts of PAS(+)-positive mesangial matrix, glomerulosclerosis, dilatation of the urinary space and thickening of basement membranes in glomeruli.