[Effect in vitro of albendazole on the kinetics of cytosolic glutathione transferase from the rat liver].

Introduction: Since the idea of multifunctional mode of action of anthelmintics is considered and in experimental trichinellosis in vivo albendazole seems to act as an allosteric activator of cytosolic GST from mice muscles, in this study a termosensitivity after in vitro incubation with albendazole of purified commercial cytosolic glutathione transferase (GST) from the rat liver was investigated.

Methods: Two extremal temperatures: -80 degrees C and +30 degrees C were used to destroy the dimer in quaternary structure of this enzyme.

Results: In control preparations both extremal temperatures destroy this structure, so the Michaelis-Menten kinetic curves of substrate saturation show the typical hyperbolic shape.

Evaluation of effects of albendazole on the kinetics of cytosolic glutathione transferase in skeletal muscles during experimental trichinellosis in mice.

In this study, the effect of trichinellosis as well as the effect of albendazole treatment on the kinetics of substrate saturation of cytosolic glutathione transferase (GST) in the skeletal muscles from infected mice was examined because the idea of multifunctional mode of action of anthelmintic drugs was considered. Our results pointed out to the influence of trichinellosis and albendazole treatment on the quaternary structure of GST in mouse muscles. A double reciprocal plot of GST saturation in control mice was biphasic with apparent low and high K (m) values equal to 0.

[The data from the first investigations of a new antioxidants family--peroxidoxins--in parasites].

The properties and mechanism of action of a new family of antioxidants-peroxidoxins-have been described. The properties of parasitic peroxidoxins in some helminths and protozoans discovered as a result of genom sequencing and expressed sequence tag (EST) project have been presented. The data described untill now from a number of studies with parasites indicate that the family of peroxidoxins, very important for the host-parasite interactions may be in future a potential candidate vaccine molecules in some systems.

[The intriguing influence of albendazole on glutathione reductase activity in the muscles from Trichinella spiralis infected mice].

A clear stimulation of the activity of glutathione reductase (RG, EC 1.6.4.

Effect of aminoguanidine and albendazole on inducible nitric oxide synthase (iNOS) activity in T. spiralis-infected mice muscles.

The aim of this study was to provide evidence for the expression of iNOS in the cells of inflammatory infiltrates around larvae in skeletal muscles of T. spiralis infected mice. The BALB/c mice (n = 8) divided into subgroups, received either aminoguanidine (AMG)--a specific iNOS inhibitor or albendazole (ALB)--an antiparasitic drug of choice in trichinellosis treatment.

Changes in the activity of glutathione-S-transferase in muscles and sera from mice infected with Trichinella spiralis after treatment with albendazole and levamisole.

In this study, changes in GST activity before and after treatment with antiparasitic drugs were measured in muscles and sera from mice infected with Trichinella spiralis between the 2nd and 9th weeks post-infection (wpi). Levamisole and albendazole, two anthelmintics with a different mode of action, were used. An about twofold stimulation of GST activity in the 2nd wpi and in the later muscular phase (6-7 wpi) was observed in muscles from animals treated with albendazole.