Using rattlesnake microsatellites to determine paternity in captive bushmasters (Lachesis muta).

We used 10 microsatellite DNA markers originally described for two Crotalus and one Sistrurus species to infer paternity in a captive-hatched clutch of Lachesis muta. Although the dam was known, records listed two potential sires, which prevented the inclusion of those offspring in a captive breeding program. Samples were collected from both possible sires, the presumed dam and two offspring.