Isozymes of AMP-Deaminase in Muscles Myasthenia Gravis Patients.

Similar symptoms observed in Myasthenia gravis (MG) can be also detected in the case of skeletal muscle AMP-deaminase deficiency. We compared the activity and expression of - products in skeletal muscles of MG patients and MG-free individuals. The activity of AMP-deaminase in the muscles of MG patients was significantly higher than in the controls and was 2.

Paraquat inhibits progesterone synthesis in human placental mitochondria.

Introduction: Human placenta mitochondria produces huge amounts of progesterone necessary for maintaining the pregnancy. Lipid peroxidation in human placental mitochondria inhibits progesterone synthesis and that inhibition can be reversed by superoxide dismutase and other antioxidants. Paraquat (PQ) a highly toxic herbicide generates superoxide radical inside cells and induces lipid peroxidation.

Effect of AMP-deaminase 3 knock-out in mice on enzyme activity in heart and other organs.

Recent findings suggest that inhibition of AMP-deaminase (AMPD) could be effective therapeutic strategy in heart disease associated with cardiac ischemia. To establish experimental model to study protective mechanisms of AMPD inhibition we developed conditional, cardiac specific knock-outs in Cre recombinase system. AMPD3 floxed mice were crossed with Mer-Cre-Mer mice.

[Acute poisoning with sertindole--a case report].

The paper presents a case of acute, accidental sertindole poisoning. Intoxication had a stormy clinical course with symptoms of cardiovascular, respiratory and nervous system. A relatively small dose of ingested preparation and severe overdose course may indicate a low therapeutic drug index.

[Electronic poison information management system].

We describe deployment of electronic toxicological information database in poison control center of Pomeranian Center of Toxicology. System was based on Google Apps technology, by Google Inc., using electronic, web-based forms and data tables.

AMP deaminase 1 gene polymorphism and heart disease-a genetic association that highlights new treatment.

Nucleotide metabolism and signalling is directly linked to myocardial function. Therefore analysis how diversity of genes coding nucleotide metabolism related proteins affects clinical progress of heart disease could provide valuable information for development of new treatments. Several studies identified that polymorphism of AMP deaminase 1 gene (AMPD1), in particular the common C34T variant of this gene was found to benefit patients with heart failure and ischemic heart disease.

Accidental intoxication with high dose of methoxetamine (MXE)--a case report.

Unlabelled: Methoxetamine (MXE) is an analogue of ketamine.

Case Report: We present a 25-year-old male who, after getting an information from the Internet, started to use MXE to avoid the excitement connected with recreational codeine abuse. For about 8 - 10 months he injected about 100 mg of MXE intramuscularly.

[Manganism--neurodegenerative brain disease caused by poisoning with manganese].

Manganism is a neurodegenerative disease of brain caused by intoxication by manganese and its excessive accumulation in this tissue. Some of the clinical symptoms observed in this disease resemble these observed in Parkinson disease.

[Metabolic acidosis--cause of sudden, unexplained death among chronic alcohol abusers].

Metabolic acidosis (lactic and ketoacidosis) are frequent cause of sudden, unexplained deaths of alcohol abusers with negative results of pathomorphological and toxicological determinations. We described the pathomechanism leading to the acidosis.

[Reactive oxygen species and 3,4-dihydroxyphenylacetaldehyde in pathogenesis of Parkinson disease].

Reactive oxygen species, which plays a role in pathogenesis of many neurodegenerative diseases, seems to be important also in pathogenesis of the Parkinson's disease. Experiments performed recently, revealed in the cerebrum of patients suffering from this disease (induced by the oxidative stress) elevated levels of 3,4-dihydroxyphenylacetaldehyde (DOPAL)--a strong endogenous neurotoxin to dopamine neurons.

Melatonin enhances antioxidant action of alpha-tocopherol and ascorbate against NADPH- and iron-dependent lipid peroxidation in human placental mitochondria.

Human placental mitochondria might be a significant source of NADPH- and iron-dependent production of reactive oxygen species (ROS). Preeclampsia is believed to be a consequence of overproduction of ROS in human placenta. The experimental results presented here show that melatonin inhibits NADPH- and iron-dependent lipid peroxidation of human placental mitochondria in a concentration-dependent manner.

[Pathomechanism of vitamin D intoxication].

Pathomechanism of vitamin D intoxication is still unclear. Experiments on animals showed that intoxication is accompanied by distinctly elevated serum level of vitamin D precursor [25(OH)D3] but not by that of hormonally, metabolic active form of it [1alpha,25(OH)2D3]. The most probable explanation of this phenomenon is that vitamin D precursor releases hormonal form of vitamin from its binding with serum transporting protein (DBP) stimulating in this way transcription of genes in cells of target tissues.

[Energy metabolism in the failing heart].

Daily heart consumption of energy (about 6 kg of ATP) exceeds significantly energy consumption of other organs. Deprivation of energy is considered as one of the main factors in development of heart failure. Currently available research methods permit not only to evaluate more precisely the role of impaired energy metabolism in the heart failure, but also give a hope for the future metabolic therapy.

[Hypercholesterolaemia among patients of the cardiology ward of a Regional Hospital in Wejherowo].

The strong positive relationship between cholesterolaemia and ischaemic heart disease is unquestioned and comes from several lines of evidence. In this paper results of questionnaire studies concerning cholesterolemia performed among patients of the Cardiology Ward of a Regional Hospital in Wejherowo are presented.

[Weak anti-inflammatory effects of acetaminophen are related with its free radicals scavenger activity].

The main target of acetaminophen application is bifunctional enzyme--prostaglandin endoperoxide H2 synthase (PGHS)--which has cyclo-oxygenase and peroxidase activities and synthesizes initial intermediates in prostanoid synthesis. The reaction catalyzed by PGHS is radical-based and it is initiated and then maintained by the constant presence of peroxides especially peroxynitrate, which generate so-called "peroxide tone" in the enzyme surrounding. Currently it is known that inhibitory effect of acetaminophen on PGHS activity is directly connected with the elimination of "peroxide tone".

[Significant periodical fluctuation of insulin requirement in patients with type 2 diabetes].

We have described three physicians aged 65-70 years, who had noticed during their insulintherapy significant, periodically recurrent variations of insulin requirement (max. 50 i.u.

NADPH- and iron-dependent lipid peroxidation inhibit aromatase activity in human placental microsomes.

During pregnancy placenta is the most significant source of lipid hydroperoxides and other reactive oxygen species (ROS). The increased production of lipid peroxides and other ROS is often linked to pre-eclampsia. It is already proved that placental endoplasmic reticulum may be an important place of lipid peroxides and superoxide radical production.

Expression patterns of AMP-deaminase and cytosolic 5'-nucleotidase genes in human term placenta.

Background AMP-deaminase (EC 3.5.4.

[The role of intestine in detoxification].

In the result of liver detoxification, xenobiotics change into more water soluble and thus easier for excretion from the body. It is convenient to consider this process as occurring in two phases. In phase I, the major reactions involved are hydroxylation, catalyzed by monoxygenases.

[Heme oxygenase as a potential therapeutic target for hepatoprotection].

Heme oxygenase (HO) is the rate limiting enzyme in the breakdown of heme into carbon monoxide (CO), iron and biliverdin. To date three mammalian HO isozymes have been identified, and only one of them (HO-1) is inducible. The products of HO-catalyzed reaction, particularly CO and biliverdin/bilirubin have been shown to exert protective effects in the liver against different noxious stimuli.

Do pancreatic endocrine stem cells exist?

After the transplantation of hearts from female donors into male recipients, cells migrating from the host to the transplanted heart demonstrate the presence of the Y chromosome (up to 18 percent of all cardiomyocytes). Most probably, this kind of migration occurs in all transplantations. However, postnatal origins of pancreatic beta-cells remain still controversial.

Carbon monoxide--a regulator of vascular tone in hypoxia?

Carbon monoxide is a side product of enzymatic degradation of heme--a reaction catalyzed by heme oxygenase (HO). There are three independent molecular forms (isozymes) of heme oxygenase in man. Isoforms HO-2 and HO-3 are constitutive.

AMP-deaminase from normal and cirrhotic human liver: a comparative study.

AMP-deaminase (EC 3.5.4.

AMP-deaminase from hen stomach smooth muscle--physico-chemical properties of the enzyme.

AMP-deaminase from hen stomach smooth muscle was isolated and physico-chemical properties of the purified enzyme were investigated. The enzyme had an activity optimum at pH 6.5, and poorly deaminated the substrate analogues tested.