Publications by authors named "Krupnik O"
The review considers the properties and some applications of complexes formed by peptide nucleic acid (PNA) molecules with complementary DNA sites, with emphasis on the structure and properties of PNA2/DNA triplexes.
View Article and Find Full Text PDFStability and transformations of bis-PNA/DNA triplex structural isomers.
J Biomol Struct Dyn
February 2004
Article Synopsis
- The study focuses on the formation of structurally isomeric complexes between homopyrimidine bis-PNAs and different forms of DNA (single- and double-stranded).
- Isomers S1, S2, and S3 are identified based on their mobility in polyacrylamide gel electrophoresis, with S3 being the most stable and forming exclusively on double-stranded DNA.
- The research also finds that the stability of isomer S1 significantly increases when an excess of complementary single-stranded oligonucleotide is present, and proposes that the S1 structure consists of two bis-PNA/DNA triplexes.
Equilibrium melting curves were obtained for triplexes, formed by single stranded DNA containing an A10 target with bis-PNA consisting of two T10 decamers. Thermodynamic parameters of melting were determined for Na(+) concentrations 50, 200 and 600mM by two methods. The melting enthalpy Delta H degrees was evaluated from the width of the differential melting curves and from the concentration dependence of the melting temperature.
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