[Mooren's ulcer].

The article reviews available data on Mooren's ulcer - a rare disease of, supposedly, autoimmune origin that manifests as peripheral ulceration of the cornea and poses a risk of its perforation. The variability of clinical presentation and course of the disease often leads to misdiagnosis and inadequate treatment. The present review focuses on specific signs characteristic of the disease and the most effective methods of its treatment.

[INDICES OF THE OXIDATIVE STATUS IN CHRONIC ADMINISTRATION OF COLLOID CARBONATE CALCIUM PRAPARATION WITH FAUCET AND LOW-MINERALIZED DRINKING WATER IN RATS].

There are discussed the changes of an array of indices of the oxidative status in chronic administration of colloidal calcium carbonate preparation with faucet and low-mineralized drinking water to rats. Slight differences between significant effects of administration of 3 and 30 mg/L of preparation permit to suggest that the process of its incoming delivery into organism of rats has a bottleneck in the nature of total capacity of macrophages of intestinal lymphoid tissue to absorption of particles.

[Biochemical effects of chronic peroral administration of carbon nanotubes and activated charcoal in drinking water in rats].

Chronic 6-month experiment was carried out in rats, which received drinking water with multi-walled carbon nanotubes (MWCNTs), diameter of 15-40 nm, length ≥ 2 mkm) or activated charcoal (AC, diameter of 10-100 mkm), blood samples of the animals were used for assessment of biochemical markers. Both coal compounds induced the appearance of signs of oxidative stress 2 weeks after the beginning of the experiment and alteration of serum markers of liver and renal damage, as well as changes of cortisol and protein serum concentrations later Thus, despite of known high (asbest-like) inhalation toxicity of carbon nanotubes in comparison with other carbon allotrops (fullerenes and black carbon), we have found similar effects of MWCNTs and carbon microparticles in orally treated rats.

[Comparative analysis of the influence of nano- and ionic forms of silver on biochemical indices in laboratory animals].

A comparative analysis of the effect of silver nanoparticles (SNP) with a diameter of 14 nm and silver sulfate (SS) on biochemical indices of the state of the organism under the administration of preparations with drinking water to mice F1 CBAxC57Bl (from 0.1 to 500 mg Ag/l for 2 weeks) and nonlinear rats (from 0.01 to 5 mg Ag/l within 6 months) has been performed In the experiment on mice there was found an increase in activity of glutathione reductase (GR) in erythrocytes under the administration of both drugs and reduction of antiradical activity of serum--under the introduction of SNP only.

[Phenotypic polymorphism of biomedical parameters of the health status in hygienic studies].

The paper presents the results of investigating the phenotypic polymorphism of a number of biochemical and immunological parameters (the values of oxidative stress, the activity of catalase and glutathione-S-transferase in red blood cells, the serum levels of catecholamines, tumor-necrosis factor-?, and IgG antibody subclasses) in the authors' hygienic studies of genotypic and nongenotypic population samples.

FTIR spectroscopy studies of nucleic acid damage.

Fourier transform infrared (FTIR) spectra of RNA isolated from tumour brain (glioma) and DNA isolated from low-dose gamma-irradiated epididymis cells of rats from the Chernobyl accident zone have been investigated in the 700-4000 cm(-1) region. The total absorbed radiation doses were equal to or less than 57 cGy. Observed changes in the FTIR spectra represent the damage in the primary, secondary and tertiary structure of nucleic acid, which seem to be connected with modification of bases and sugars, and redistribution of the H-bond network.

[Unusual shapes of absorption isotherms in three-component systems].

It has been shown that the shape of Scatchard isotherms upon competitive binding of two ligands to the same binding site in the three-component ligand 1-ligand 2-DNA system depends crucially on the binding constant values. The binding isotherm of ligand 2 in the presence of the competitive ligand 1 turns back (has a bow-like form) when the binding constant of the first ligand is larger than the binding constant of the second one.

[Interaction of actinocin derivative with different poly(rC) structures].

The interaction of actinocin derivative Act III with single- and double-stranded poly(rC) has been investigated by the methods of differential scanning microcalorimetry and UV-vis absorption spectroscopy. It was shown that, after the addition of the ligand, the temperature, enthalpy and entropy of poly (rC) melting decrease. The analysis of poly(rC)-ActIII absorption spectra indicated that the conformation of polynucleotide differs from that of free poly (rC) in the presence of ActHI at pH 4.

[Berberine and amitozine binding with calf thymus DNA].

Binding of amitozine and berberine to DNA has been investigated by VIS- and UV-spectroscopy. It has been found that amitozine forms one type of complex and berberine forms two types of complexes with DNA. Observed concentration dependences of absorption spectra were analyzed using the DALSMOD optimization program and association constants were calculated (K(BCl)= 3 x 10(3) M(-1), K(Am) = 1.

[Spatial ultrasound visualization of the eye and orbit].

The paper presents data on the diagnostic use of three-dimensional (3-D) ultrasound visualization, analyzes earlier existing and new ultrasound diagnostic technologies, and shows it expedient to apply them in ophthalmologic practice. A hundred and forty-six healthy individuals and 52 patients (60 eyes) with different diseases of the eye and orbital cavity (chorioidal melanoma, facial angiomatosis, retinal detachment, vitreoretinal changes, etc.) were examined.

[The use of the spectrophotometric analysis for the calculation of the thermodynamic parameters in actinocin derivative-DNA systems].

The spectral properties of the actinocin derivative ActII in complexes with DNA were studied by UV visible spectrophotometry. Two binding models with one and two binding sites for competitive binding with different values of parameters were considered. To choose an optimal model of complexation, the optimization program of spectrophotometric concentration dependencies DALSMOD was used.

[2. Diagnostic resources and clinical indications].

Preliminary investigations, dedicated to the diagnostic application of three-dimension ultrasonic angioreconstruction, confirmed the feasibility of using the above method in ophthalmologic practice in cases of suspected intraocular and orbital neoplasms. 32 patients (37 eyes) with various pathologies of the eye and orbital cavity, including choroidal melanoma, facial angiomatosis, retinal detachment, chronic uveitis etc.) were examined.

[Three-dimensional computerized sonography in evaluation of the vascular system of the eye and orbit. I. Methodological approach and principles of analysis of results].

An examination of 40 healthy persons (80 eyes) enabled a refinement and unification of the ultrasound techniques applicable to investigations of a. ophthalmica, a. and v.

[Spectrophotometric complexes of nucleic acids with pyronine G dyes as a test for radiation damage of DNA].

The spectrophotometric titration curves of the complexes of polynucleotide matrix with pyronine G are highly specific depending on the type and conformation of polyelectrolyte (native and denatured DNA, polyribonucleotides, tRNA). It is found that DNA from the liver of experimental animals of different age, permanent inhabitants of the Chernobyl zone, forms complexes with RNA. A method for testing the defects in a DNA secondary structure have been developed based on comparative analysis of the spectra for the complexes of different nucleic acids with dyes and relative curves of spectrophotometric titration.

[Mechanisms of anti-inflammatory activity of antioxidants in lung tuberculosis].

The experiment on 120 non-inbred albino rats with induced pulmonary destructive tuberculosis has established that the antiinflammatory action of the antioxidant dibunol is displayed through changes in the ratio of biologically active 11-hydroxycorticosteroids--free hydrocortisone and corticosterone.

[Experimental and theoretical studies of the formation of DNA complexes with biologically active ligands containing chromophore groups, depending on the ionic strength of the solution].

The binding of two ligands--dyes pyronin G (PG) and methylene blue (MB)--to DNA depending on the Na+ concentration was studied spectrophotometrically in the visible and UV ranges. The theoretical model taking into account the binding of a dye to DNA by means of aggregation and intercalation on two independent lattices was used for computation of the complexation parameters. The results of optimization of spectrophotometric concentration dependences show that the ligand occupies two phosphates in the process of aggregation on a negatively charged matrix.

[Biochemical mechanisms of neurotoxic reactions to antibacterial preparations in patients with pulmonary tuberculosis].

Examination comprising the electrophysiologic method and study of the biochemical parameters of the antioxidant/lipid peroxidation system and metabolism of biologic amines in 163 patients with infiltrative pulmonary tuberculosis has revealed a clear interaction existing between the functional changes of the CNS state, the state of antioxidative defense (alpha-tocopherol) and serotonin. This allows an assumption that the antioxidant/lipid peroxidation system and serotoninergic system actively participate in the pathogenesis of development of neurotoxic reactions to antibacterial preparations in the clinical picture of tuberculosis.

[Lymphocyte enzymes, activity of lipid peroxidation processes and the antioxidant protection of patients with tuberculosis of the lungs].

Activity of succinate dehydrogenase, alpha-glycerophosphate dehydrogenase, NAD2- and NADP2-diaphorases and acid phosphatase in lymphocytes of the peripheral blood as well as malonic dialdehyde and alpha-tocopherol, as parameters of lipid peroxidation defense, were studied in 49 patients with different forms of pulmonary tuberculosis and in 17 practically and clinically healthy subjects. Patients with focal pulmonary tuberculosis presented drop of succinate dehydrogenase and NAD2-diaphorase activity and rise of alpha-glycerophosphate dehydrogenase and NADP2-diaphorase activity in lymphocytes. Parameters of malonic dialdehyde and alpha-tocopherol in patients and healthy subjects had no difference.

[Possibilities of intravenous use of helium-neon laser in the treatment of experimental tuberculosis of animals].

The study presents experimental finding of 40 mongrel dogs whose intravascular blood was irradiated with laser as a supplement to the multimodality treatment of respiratory tuberculosis. Earlier disappearance of intoxication symptoms and reduced terms of destruction cavity decrease and closure, as roentgenologically evidenced, was achieved. The influence of this treatment on certain lipid peroxidation parameters, hemocoagulation, immunity status and bacteriostatic blood activity were found.

[Functional disorders of the central nervous system in patients with pulmonary tuberculosis].

Two groups of pulmonary tuberculosis patients were followed up to reveal the relation between the lipid peroxidation (LPO) processes and the function of the central nervous system (CNS) in pulmonary tuberculosis. Group 1 (n = 76) included subjects with normal baseline electroencephalographic findings; group 2 (n = 41) consisted of those with profound changes in the electroencephalogram. The findings of LPO processes were assessed by the blood levels of malonic dialdehyde and alpha-tocopherol.

[The pro-fibrotic and pro-oxidant action of isoniazid].

As a result of experimental research on rats with destructive pulmonary tuberculosis, oxidative action of isoniazid in a dose of 50 mg per kg body weight, corresponding to the mean therapeutic dose of 10 mg/kg in man, was clearly defined. Fibrotic action of the drug was also shown. It manifested itself by a sharp rise of insoluble collagen levels in the lung tissue tuberculous lesion focus of the treated animals and in the formation of mature connective tissue.

[Changes in the levels and composition of glycolipids in various sections of the spinal cord of patients with spinal tuberculosis].

The post mortem content and composition of cerebrosides, sulfocerebrosides and gangliosides found in different sections of the spinal marrow of the healthy persons and 5 patients with spinal tuberculosis were analysed. It was shown that the changes in the content of gangliosides were varying in the lesion focus of different patients. In all patients with chronic tuberculous spondylitis, a significant drop in the content of cerebrosides (myelin markers) and sulfocerebrosides in the lesion focus, as compared with controls, was detected.

[Changes in the content and composition of phospholipids in various portions of the spinal cord in patients with tuberculosis of the spine].

Content and composition of phospholipids in various sections of the spinal marrow were investigated in patients with spinal tuberculosis. In the patients with tuberculous spondylitis, changes in the content of phospholipids in the spinal marrow were observed which were especially pronounced in the injured areas. The changes varied in different patients: in some patients, the content of the lipids was changed insignificantly while in the others it was markedly lowered (5-6-fold).

[Mechanism of the hypotensive effect of sodium oxybutyrate in pulmonary hypertension in experimental tuberculosis in dogs].

The mechanism of sodium oxybutyrate effect on the lung vessels under normal conditions and under conditions of experimental tuberculosis in dogs was studied by the time course of pressure in the pulmonary artery and the content of serotonin, histamine and adenosine in blood of the aorta and pulmonary artery after the drug intravenous administration. To confirm the antihypoxic effect of sodium oxybutyrate, the levels of lactate, pyruvate, ATP, ADP and AMP were investigated. The levels of adenosine were judged indirectly by the activity of 5-nucleotidase, adenosine desaminase and AMP.