[Use of immunoenzyme assay to detect the level of pepsinogen in blood].

Determining the level of blood pepsinogens has important clinical significance in the formation of risk groups for gastric cancer and erosive/ulcerative lesions of the gastric mucosa. A comparison of imported and domestic kits for testing the level of pepsinogens was performed. Russian diagnostic kits in comparison with foreign ones proved to show similar sensitivity and specificity.

[The level of serum cytokines in children infected with various strains of Helicobacter pylori].

Serum contents of 7 cytokines (IL-1, IL-1beta, IL-6, IL-8, TNF-alpha, INF-gamma, INF-alpha) were examined in 87 children aged 14-17 years according to presence of antibodies against Helicobacter pylori and cytotoxic CagA protein. There was a lack of difference in cytokine levels between infected and non-infected children. Thus H.

[Prevalence of atrophic gastritis in different populations in Siberia on medical evidence of the serological survey].

Prevalence of atrophic gastritis in various population of Siberia with serological tests was studied. Representative samples of Novosibirsk adult population and also urban and rural population of Yakutia were examined. 348 persons at the age more than 45 years (180 males and 168 females) were studied.

[Noninvasive diagnosis of chronic atrophic gastritis by serology].

The authors describe a current approach to the laboratory diagnosis of chronic gastritis, by using the plates of serological tests: pepsinogen I (PG-I), gastrin 17, and antibodies to Helicobacter pylori (HP). These tests and a questionnaire were used to examine 168 persons aged 45-70 years, who were a random population sample. Almost a fourth of the adult population was observed to have pronounced gastric mucosal atrophic changes, which might be associated with the high prevalence of HP infection.

[Helicobacter pylori and symptoms of gastroesophageal reflux in adolescents: role of cagA-comprising strains].

The objective of this study was to assess the prevalence of gastroesophageal reflux symptoms (GERS) in 403 adolescents at the age of 14-17 according to the Helicobacter pylori status in a two-year prospective school-based survey. Initially the H. pylori infection was revealed in 55.

[Relationship between CagA-bearing strains of Helicobacter pylori and gastrointestinal pathology].

Aim: To study the prevalence of CagA-positive strains of Helicobacter pylori in Novosibirsk residents and to assess their relation to some clinical parameters.

Material And Methods: Random sample of the population (218 males and 220 females aged 25-64 years) was examined. Antibodies against Helicobacter pylori and CagA protein were tested in the sera using enzyme immunoassay.

[Prevalence of Helicobacter pylori CagA-producing strains within families].

During prophylactic examination of blood sera taken from the members of 59 families by the enzyme immunoassay, antibodies to H. pylori and CagA protein were determined. As shown in this study, the children of non-infected mothers proved to be infected in 6.

[Association between the level of serum pepsinogen I and markers of the Helicobacter pylori infection in teenagers].

The concentration of pepsinogen I as well as antibodies to Helicobacter pylori and CagA protein were determined in the blood serum in 84 schoolchildren at the age of 14-17 using the immune-enzyme analysis. The level of pepsinogen I did not depend on the sex, age, gastroenterological symptoms and ulcer occurrence in parents. However, the concentration of pepsinogen was higher in teenagers infected with H.

[The detection of antibodies to the trophozoite antigens of Lamblia by an immunoenzyme method].

Whether purified antigens of Lamblia intestinalis trophozoites can be used to detect these antibodies by immunoassay. The drugs of immunodominant Lamblia antigens were prepared by anion-exchange chromatography of solubilized trophozoite components and they are mainly presented by proteins having molecular weights of 70, 56, and 49 kD. Immunoassay using these antigens revealed antibodies to Lamblia trophozoite antigens in sera of 87.