Publications by authors named "Krisztina Christmon"

Article Synopsis
  • * In 2021, researchers analyzed RNA viromes from a significant number of honey bees and mites, uncovering 45 complete and 1702 partial viral genomes, leading to the identification of new viral entities.
  • * Common viruses identified included Sinaivirus and various strains of Iflavirus, with concerns that existing detection methods might misrepresent the presence of certain virus types in honey bee populations.
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Novel transmission routes can allow infectious diseases to spread, often with devastating consequences. Ectoparasitic varroa mites vector a diversity of RNA viruses, having switched hosts from the eastern to western honey bees ( to ). They provide an opportunity to explore how novel transmission routes shape disease epidemiology.

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Background: Managed honey bees are key pollinators of many crops and play an essential role in the United States food production. For more than ten years, beekeepers in the United States have been reporting high rates of colony losses. One of the drivers of these losses is the parasitic mite Varroa destructor.

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We developed a honey bee RNA-virus vector based on the genome of a picorna-like Deformed wing virus (DWV), the main viral pathogen of the honey bee (). To test the potential of DWV to be utilized as a vector, the 717 nt sequence coding for the enhanced green fluorescent protein (eGFP), flanked by the peptides targeted by viral protease, was inserted into an infectious cDNA clone of DWV in-frame between the leader protein and the virus structural protein VP2 genes. The in vitro RNA transcripts from -tagged DWV cDNA clones were infectious when injected into honey bee pupae.

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