Publications by authors named "Krisztina Birone Barna"
Embryonic limb bud-derived micromass cultures are valuable tools for investigating cartilage development, tissue engineering, and therapeutic strategies for cartilage-related disorders. This collection of fine-tuned protocols used in our laboratories outlines step-by-step procedures for the isolation, expansion, and differentiation of primary mouse limb bud cells into chondrogenic micromass cultures. Key aspects covered in these protocols include synchronized fertilization of mice (Basic Protocol 1), tissue dissection, cell isolation, micromass formation, and culture optimization parameters, such as cell density and medium composition (Basic Protocol 2).
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- Much of the skeletal system develops through endochondral ossification, particularly in early fetal life, making it difficult to study initial chondrogenesis in living organisms.
- Researchers are highly interested in improving methods for culturing chondrogenic cells to effectively regenerate articular cartilage and restore joint function.
- The article outlines various protocols, including a micromass culture system for chick embryo-derived cells, high-efficiency cell transfection, and histochemical detection techniques for assessing cartilage matrix production.