Publications by authors named "Kristyna Barvikova"
Background: In animals, dietary sulfur amino acid restriction (SAAR) improves metabolic health, possibly mediated by altering sulfur amino acid metabolism and enhanced anti-obesogenic processes in adipose tissue.
Aim: To assess the effects of SAAR over time on the plasma and urine SAA-related metabolites (sulfurome) in humans with overweight and obesity, and explore whether such changes were associated with body weight, body fat and adipose tissue gene expression.
Methods: Fifty-nine subjects were randomly allocated to SAAR (∼2 g SAA, n = 31) or a control diet (∼5.
Background: Dietary sulfur amino acid restriction (SAAR) improves metabolic health in animals. In this study, we investigated the effect of dietary SAAR on body weight, body composition, resting metabolic rate, gene expression profiles in white adipose tissue (WAT), and an extensive blood biomarker profile in humans with overweight or obesity.
Methods: N = 59 participants with overweight or obesity (73% women) were randomized stratified by sex to an 8-week plant-based dietary intervention low (~ 2 g/day, SAAR) or high (~ 5.
Article Synopsis
- Regulation of hydrogen sulfide (HS) homeostasis in humans is not well understood, prompting a study of patients with rare enzyme deficiencies related to HS synthesis and catabolism.
- Analysis of sulfur compounds in these patients revealed unexpected results, such as increased bioavailable sulfide levels in those with cystathionine β-synthase (CBS) deficiency, suggesting compensatory mechanisms at play.
- The study highlights the complexity of HS regulation, showing that various genetic defects can lead to altered levels of sulfur compounds, underscoring the need for a thorough understanding of HS homeostasis in metabolic disorders.
The Transient Receptor Potential Ankyrin 1 (TRPA1) channel is an integrative molecular sensor for detecting environmental irritant compounds, endogenous proalgesic and inflammatory agents, pressure, and temperature. Different post-translational modifications participate in the discrimination of the essential functions of TRPA1 in its physiological environment, but the underlying structural bases are poorly understood. Here, we explored the role of the cytosolic N-terminal residue Ser602 located near a functionally important allosteric coupling domain as a potential target of phosphorylation.
View Article and Find Full Text PDFOur understanding of the general principles of the polymodal regulation of transient receptor potential (TRP) ion channels has grown impressively in recent years as a result of intense efforts in protein structure determination by cryo-electron microscopy. In particular, the high-resolution structures of various TRP channels captured in different conformations, a number of them determined in a membrane mimetic environment, have yielded valuable insights into their architecture, gating properties and the sites of their interactions with annular and regulatory lipids. The correct repertoire of these channels is, however, organized by supramolecular complexes that involve the localization of signaling proteins to sites of action, ensuring the specificity and speed of signal transduction events.
View Article and Find Full Text PDFArticle Synopsis
- TRPA1 is a critical channel for sensing reactive compounds and shows different sensitivities to temperature in various species due to evolutionary changes.
- Mouse TRPA1 is involved in detecting both noxious cold and heat, while human TRPA1 has been found to be a bidirectional thermosensor that can respond to both temperatures.
- The study compares human and mouse TRPA1 properties, revealing that they are activated by heat and cold in different ways and suggesting an allosteric mechanism for their temperature responsiveness variability.