Publications by authors named "Kristina Kovacova"

The dataset consists of records from the NTFS file system and event logs. In this study, we used images of devices from capture the flags competitions focused on the digital forensic of Windows operating systems and user activities. We created timelines of the security incident from the disk images using the Plaso tool, which we then processed and transformed the attributes of the timelines into binary values to simplify the application of data analysis and machine learning methods.

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Article Synopsis
  • Polysaccharide endotransglycosylases (PETs) are enzymes crucial for modifying the cell walls of fungi and plants, playing a key role in how these organisms grow and respond to infections.
  • Inhibiting PETs could be an effective strategy for treating fungal infections, making them a target for drug development.
  • The study describes two innovative high-throughput assays to identify PETs and screen their inhibitors, using different methods to separate labeled products from unreacted components in microplate setups.
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Fungal wall formation is a dynamic process involving several categories of enzymes. The GH72 family of β(1,3)-glucanosyltransferases is essential for the determination of cell shape, for cell integrity and for virulence in pathogenic fungi. Candida albicans has five GH72 genes: PHR1 and PHR2 are pH dependent, the first being expressed at pH ≥ 6 and repressed at lower pH and the second regulated in the opposite manner, PGA4 is transcribed independently of pH whereas PHR3 and PGA5 have low expression levels.

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The mechanical properties of fungal cell walls are largely determined by composition and mutual cross-linking of their macromolecular components. Previous work showed that the Crh proteins are required for the formation of cross-links between chitin and glucan at the Saccharomyces cerevisiae cell wall. In the present study, the proteins encoded by CRH1 and CRH2 were heterologously expressed in Pichia pastoris and a sensitive fluorescence in vitro soluble assay was devised for determination of their transglycosylating activities.

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