Publications by authors named "Kristina K Bushkova"
Article Synopsis
- This study investigates the ability of specific protein fragments (2D FN3 and CD FN3) from a bacterial strain, GT15, to bind to the inflammatory cytokine TNF-α, using a sandwich ELISA method for detection.
- Results reveal that neither 2D FN3 nor CD FN3 can bind to TNF-α alone, however, the full ∆FN3.1 protein is capable of forming a binding pocket for TNF-α.
- Additionally, the study analyzes genetic variations in the amino acid sequences of ∆FN3.1 and compares FN3 domains from human gut bacteria, highlighting those with cytokine receptor motifs and their structural similarities with human proteins.
Most species of the genus Bifidobacterium contain the gene cluster PFNA, which is presumably involved in the species-specific communication between bacteria and their hosts. The gene cluster PFNA consists of five genes including fn3, which codes for a protein containing two fibronectin type III domains. Each fibronectin domain contains sites similar to cytokine-binding sites of human receptors.
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