Soluble plant cell signals induce the expression of the type III secretion system of Pseudomonas syringae and upregulate the production of pilus protein HrpA.

Type III protein secretion is essential for the pathogenicity of Pseudomonas syringae on its host plants. Expression of HrpA, a major component of the type III secretion system (T3SS)-associated pilus, was studied both in plant leaves and in vitro using reporter genes. We found that induction of the hrpA promoter was stronger in plants than in vitro, and that the induction was enhanced by both host and nonhost plants of P.

Bioremediation of diesel oil-contaminated soil by composting with biowaste.

Soil spiked with diesel oil was mixed with biowaste (vegetable, fruit and garden waste) at a 1:10 ratio (fresh weight) and composted in a monitored composting bin system for 12 weeks. Pure biowaste was composted in parallel. In order to discern the temperature effect from the additional biowaste effect on diesel degradation, one recipient with contaminated soil was hold at room temperature, while another was kept at the actual composting temperature.