Investigation of the HIV-1 matrix interactome during virus replication.

Purpose: Like all viruses, human immunodeficiency virus type 1 (HIV-1) requires host cellular factors for productive replication. Identification of these factors may lead to the development of novel cell-based inhibitors.

Experimental Design: A Strep-tag was inserted into the C-terminus of the matrix (MA) region of the HIV-1 gag gene.