Use of a portable real-time reverse transcriptase-polymerase chain reaction assay for rapid detection of foot-and-mouth disease virus.

Objective: To evaluate a portable real-time reverse transcriptase-polymerase chain reaction (RT-PCR) assay designed to detect all 7 viral serotypes of foot-and-mouth disease virus (FMDV).

Design: Laboratory and animal studies.

Study Population: Viruses grown in tissue culture and animals experimentally infected with FMDV.