Glycan-Tailored Glycoproteomic Analysis Reveals Serine is the Sole Residue Subjected to -Linked Glycosylation in .

Protein glycosylation is a ubiquitous process observed across all domains of life. Within the human pathogen , -linked glycosylation is required for virulence; however, the targets and conservation of glycosylation events remain poorly defined. In this work, we expand our understanding of the breadth and site specificity of glycosylation within by demonstrating the value of strain specific glycan electron-transfer/higher-energy collision dissociation (EThcD) triggering for bacterial glycoproteomics.