DORQ-seq: high-throughput quantification of femtomol tRNA pools by combination of cDNA hybridization and Deep sequencing.

Due to its high modification content tRNAs are notoriously hard to quantify by reverse transcription and RNAseq. Bypassing numerous biases resulting from concatenation of enzymatic treatments, we here report a hybrid approach that harnesses the advantages of hybridization-based and deep sequencing-based approaches. The method renders obsolete any RNAseq related workarounds and correction factors that affect accuracy, sensitivity, and turnaround time.

Giant Two-Level Systems in a Granular Superconductor.

Disordered thin films are a common choice of material for superconducting, high impedance circuits used in quantum information or particle detector physics. A wide selection of materials with different levels of granularity are available, but, despite low microwave losses being reported for some, the high degree of disorder always implies the presence of intrinsic defects. Prominently, quantum circuits are prone to interact with two-level systems (TLS), typically originating from solid state defects in the dielectric parts of the circuit, like surface oxides or tunneling barriers.

N1-methylation of adenosine (mA) in ND5 mRNA leads to complex I dysfunction in Alzheimer's disease.

One mechanism of particular interest to regulate mRNA fate post-transcriptionally is mRNA modification. Especially the extent of mA mRNA methylation is highly discussed due to methodological differences. However, one single mA site in mitochondrial ND5 mRNA was unanimously reported by different groups.

"Real-life" data of the efficacy and safety of belantamab mafodotin in relapsed multiple myeloma-the Mayo Clinic experience.

Belantamab mafodotin is a highly selective targeted therapy for multiple myeloma. It targets the B cell maturation antigen (BCMA) on plasma cells and showed promising results in several randomized clinical trials. We report the outcomes of 36 patients treated at Mayo Clinic.

Topographic Guidance in Melt-Electrowritten Tubular Scaffolds Enhances Engineered Kidney Tubule Performance.

To date, tubular tissue engineering relies on large, non-porous tubular scaffolds (Ø > 2 mm) for mechanical self-support, or smaller (Ø 150-500 μm) tubes within bulk hydrogels for studying renal transport phenomena. To advance the engineering of kidney tubules for future implantation, constructs should be both self-supportive and yet small-sized and highly porous. Here, we hypothesize that the fabrication of small-sized porous tubular scaffolds with a highly organized fibrous microstructure by means of melt-electrowriting (MEW) allows the development of self-supported kidney proximal tubules with enhanced properties.

Manganese Ions Individually Alter the Reverse Transcription Signature of Modified Ribonucleosides.

Reverse transcription of RNA templates containing modified ribonucleosides transfers modification-related information as misincorporations, arrest or nucleotide skipping events to the newly synthesized cDNA strand. The frequency and proportion of these events, merged from all sequenced cDNAs, yield a so-called RT signature, characteristic for the respective RNA modification and reverse transcriptase (RT). While known for DNA polymerases in so-called error-prone PCR, testing of four different RTs by replacing Mg with Mn in reaction buffer revealed the immense influence of manganese chloride on derived RT signatures, with arrest rates on mA positions dropping from 82% down to 24%.

Graphical Workflow System for Modification Calling by Machine Learning of Reverse Transcription Signatures.

Modification mapping from cDNA data has become a tremendously important approach in epitranscriptomics. So-called reverse transcription signatures in cDNA contain information on the position and nature of their causative RNA modifications. Data mining of, e.

Fiber Scaffold Patterning for Mending Hearts: 3D Organization Bringing the Next Step.

Heart failure (HF) is a leading cause of death worldwide. The most common conditions that lead to HF are coronary artery disease, myocardial infarction, valve disorders, high blood pressure, and cardiomyopathy. Due to the limited regenerative capacity of the heart, the only curative therapy currently available is heart transplantation.

Mesoporous silica supported multiple single-site catalysts and polyethylene reactor blends with tailor-made trimodal and ultra-broad molecular weight distributions.

A ternary blend of the bisiminopyridine chromium (III) (Cr-1) with the bisiminopyridine iron (II) (Fe-2) post-metallocenes with the quinolylsilylcyclopentadienyl chromium (III) halfsandwich complex (Cr-3) was supported on mesoporous silica to produce novel multiple single-site catalysts and polyethylene reactor blends with tailor-made molecular weight distributions (MWDs). The preferred cosupporting sequence of this ternary blend on MAO-treated silica was Fe-2 followed by Cr-1 and Cr-3. Cosupporting does not impair the single-site nature of the blend components producing polyethylene fractions with $\overline M _{\rm w}$ = 10(4) g · mol(-1) on Cr-1, $\overline M _{\rm w}$ = 3 × 10(5) g · mol(-1) on Fe-2, and $\overline M _{\rm w}$ = 3 × 10(6) g · mol(-1) on Cr-3.

Structural changes and chain radius of gyration in cold-drawn polyethylene after annealing: small- and wide-angle X-ray scattering and small-angle neutron scattering studies.

Samples made from linear polyethylene were drawn at room temperature and subsequently annealed at high temperatures below the melting point. The structural changes of the crystalline lamellae and lamellar superstructures as well as the single chain radius of gyration were studied by means of combined small- and wide-angle X-ray scattering and small-angle neutron scattering (SANS). After drawing, the polymeric chain segments in the crystalline phase are preferentially oriented along the drawing direction with a high degree of orientation whereas the lamellae in the samples are found to be slightly sheared exhibiting oblique surfaces as evidenced by X-ray scattering.

The anthropometric verification of corrective surgery outcome in cleft secondary deformities.

An anthropometric measurement serves as both an objective assessment and a description of shape and size. Gypsum casts were used for the measurement, along with a statistical evaluation of results, to compare the appearance of patients with a complete unilateral or bilateral cleft lip and palate prior to corrective surgery of their secondary deformity and following this intervention. The evaluated approach was our own modification of rhinoplasty and lip correction, using the remodeling and extension of soft tissues envelope by a skin flap.

[2]Borametallocenophanes of Zr and Hf: synthesis, structure, and polymerization activity.

The first [2]borametallocenophanes of Zr and Hf are reported, which were obtained from 1,2-bis(dimethylamino)-1,2-dicyclopentadienyldiborane(4). The constitution of these complexes in solution and, for the Hf complex, in the crystalline state is discussed. The title complexes were studied as Ziegler-Natta-type catalyst precursors for the polymerization of ethylene.

Reconstruction of maxilla alveolus for application of dental implant in patients with cleft defect.

The prospective study of dental implant application into the reconstructed maxilla alveolus of cleft patients was started in 2001. Its aims are to specify precisely the indications, conditions and techniques, as well as the factors justifying the assumption that the results will be successful. Indication was based on an examination by a multidisciplinary therapeutic team (plastic surgeon, dental surgeon, orthodontist, and prosthetist), with the proviso that the patient should have a positive approach.

Augmentation by autologous adipose tissue in cleft lip and nose. Final esthetic touches in clefts: part I.

Although patients with a cleft lip and palate undergo many surgeries and other therapeutic procedures in the course of their treatment, many are still deeply concerned with their handicap and continue searching for perfection in their appearance. Augmentation using the subject's own fat cells involves minor invasion, is readily available, is an unpretentious method regarding time and cost, and has no contraindications. This method can serve to supplement a hypotrophic scarred upper lip and nasal columella, and by improving the volume, it induces a more natural contour, which reduces the stigmatizing deformity as well as the visibility of externally apparent scars.

Rhinomanometry in patients with a cleft deformity of the nose.

A typical cleft deformity of the central facial area is very frequently associated with impaired patency of the nose. Rhinomanometry makes it possible to assess objectively and quantitatively the patency of these airways. For objective assessment of the disorder this examination was made closely before secondary rhinoplasty in 36 patients with complete unilateral (81%) or bilateral cleft (19%).

Advantages and disadvantages of endoscopically assisted front-lift.

Reduced surgical risk, a diminution of visible scars, rapid recovery, long-termed results and reduced costs are at present the most important demands on development in aesthetic plastic surgery. Endoscopically assisted front-lift meets perfectly the need of reduced invasiveness. It is technically simple and associated with a minimum of complications.

No Polymerization with Metallocenes in Water?-A Prejudice is Refuted.

Water-sensitive catalysts work in water! Metallocene catalysts that normally have to be handled under the strict exclusion of air and moisture, for example [TiCp*(OMe) ] (1, Cp*=pentamethylcyclopentadienyl), are successfully employed in the aqueous emulsion polymerization of styrene to syndiotactic polystyrene. The catalyst is encapsulated inside the monomer droplets and thus protected against attack by water.