Publications by authors named "Kristen E Thane"
Background: A glycemic challenge test is used for the diagnosis of insulin dysregulation (ID) in horses and ponies. Different forms of the test exist where the administrative route and dose of glucose vary, which makes interpretation of results challenging.
Hypothesis/objectives: To evaluate the palatability of, and blood glucose and insulin responses to, carbohydrate pellets fed as an oral glucose test (OGT), and to establish the diagnostic threshold for ID when using the pellets.
Growing interest in extracellular vesicles (EV) has necessitated development of protocols to improve EV characterization as a precursor for myriad downstream investigations. Identifying expression of EV surface epitopes can aid in determining EV enrichment and allow for comparisons of sample phenotypes. This study was designed to test a rigorous method of indirect fluorescent immunolabeling of single EV with subsequent evaluation using nanoparticle tracking analysis (NTA) to simultaneously determine EV concentration, particle size distribution, and surface immunophenotype.
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- Mesenchymal stem cells (MSCs) have strong potential as therapeutic agents due to their ability to modulate the immune system, particularly in veterinary contexts using canine models.
- This study specifically investigates how extracellular vesicles (EVs) from Wharton's jelly-derived MSCs (WJ-MSC EV) can inhibit the proliferation of CD4 T cells, linking this effect to biochemical mechanisms involving TGF-β and adenosine.
- The findings indicate that WJ-MSC EVs exert a dose-dependent suppression of CD4 T cell proliferation, suggesting their use could be valuable for advancing immune modulation therapies in both veterinary and human medicine.
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- Myxomatous mitral valve disease (MMVD) is similar to mitral valve prolapse (MVP) in humans, but there's currently no medical treatment to slow its progression, highlighting the necessity for better understanding of its molecular pathology.
- The study analyzed exosomal miRNA from different groups of dogs (healthy, with MMVD, and with MMVD-related heart failure) to investigate potential biomarkers associated with the disease and aging.
- Significant increases in specific exosomal miRNAs (like miR-181c and miR-495) were recorded in dogs with MMVD-related heart failure, indicating that exosomal miRNA changes might be more relevant to the disease compared to total plasma miRNA.
Alpha-1 antitrypsin deficiency is typified by panacinar emphysema in humans. Whilst animal models of (α1A-TD) that more accurately reflect the histology and molecular pathology of α1A-TD are in development, it is timely to discuss methods to assess emphysema severity. Several methods exist to quantify emphysema from histologic sections, including linear mean intercept (Lm), equivalent diameters (D) or their statistical derivatives (D2), and more recently probability models of D2 ("severity index").
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