Publications by authors named "Kristel Doets"
Live-attenuated flavivirus vaccines confer long-term protection against disease, but the design of attenuated flaviviruses does not follow a general approach. The non-coding, subgenomic flavivirus RNA (sfRNA) is produced by all flaviviruses and is an essential factor in viral pathogenesis and transmission. We argue that modulating sfRNA expression is a promising, universal strategy to finetune flavivirus attenuation for developing effective flavivirus vaccines of the future.
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- Each year, millions of poultry are affected by avian influenza and infectious bursal disease, necessitating effective vaccines for prevention and control.
- Conventional vaccines often lack adequate safety and efficacy, highlighting the need for advanced vaccine platforms like self-amplifying RNA vaccines.
- This study explored Tembusu virus replicons, which showed better performance in expressing antigens and eliciting immune responses in chickens compared to the conventional Venezuelan equine encephalitis virus replicons.
The novel mosquito-borne Tembusu virus (TMUV, family Flaviviridae) was discovered as the cause of a severe outbreak of egg-drop syndrome affecting ducks in Southeast Asia in 2010. TMUV infection can also lead to high mortality in various additional avian species such as geese, pigeons, and chickens. This study describes the construction of an infectious cDNA clone of a contemporary duck-isolate (TMUV WU2016).
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