Ligand field design enables quantum manipulation of spins in Ni complexes.

Creating the next generation of quantum systems requires control and tunability, which are key features of molecules. To design these systems, one must consider the ground-state and excited-state manifolds. One class of systems with promise for quantum sensing applications, which require water solubility, are d Ni ions in octahedral symmetry.

Structurally Defined Water-Soluble Metallofullerene Derivatives towards Biomedical Applications.

Endohedral metallofullerenes (EMFs) are excellent carriers of rare-earth element (REE) ions in biomedical applications because they preclude the release of toxic metal ions. However, existing approaches to synthesize water-soluble EMF derivatives yield mixtures that inhibit precise drug design. Here we report the synthesis of metallobuckytrio (MBT), a three-buckyball system, as a modular platform to develop structurally defined water-soluble EMF derivatives with ligands by choice.

Spin-Lattice Relaxation Decoherence Suppression in Vanishing Orbital Angular Momentum Qubits.

Multifrequency electron paramagnetic resonance spectroscopy on oriented single crystals of magnetically dilute Gd(III) ions in GdY(trensal) is used to determine the Hamiltonian parameters of the ground term and its phase memory time, , characterizing its coherent spin dynamics. The vanishing orbital angular momentum of the term makes it relatively insensitive to spin-lattice relaxation mediated by magnetoelastic coupling and leads to a of 12 μs at 3 K, which is not limited by spin-lattice relaxation.

Spinning-Driven Dynamic Nuclear Polarization with Optical Pumping.

We propose a new, more efficient, and potentially cost effective, solid-state nuclear spin hyperpolarization method combining the cross-effect mechanism and electron spin optical hyperpolarization in rotating solids. We first demonstrate optical hyperpolarization in the solid state at low temperatures and low field and then investigate its field dependence to obtain the optimal condition for high-field electron spin hyperpolarization. The results are then incorporated into advanced magic-angle spinning dynamic nuclear polarization (MAS-DNP) numerical simulations that show that optically pumped MAS-DNP could yield breakthrough enhancements at very high magnetic fields.

A 9.2-GHz clock transition in a Lu(II) molecular spin qubit arising from a 3,467-MHz hyperfine interaction.

Spins in molecules are particularly attractive targets for next-generation quantum technologies, enabling chemically programmable qubits and potential for scale-up via self-assembly. Here we report the observation of one of the largest hyperfine interactions for a molecular system, A = 3,467 ± 50 MHz, as well as a very large associated clock transition. This is achieved through chemical control of the degree of s-orbital mixing into the spin-bearing d orbital associated with a series of spin-½ La(II) and Lu(II) complexes.

Case Report: A Fatal Case of Babesiosis in a Splenectomized Male Patient from Western India.

Human babesiosis is a rare disease, caused by Babesia species and commonly transmitted by tick bite. Although human babesiosis is known to be asymptomatic in immunocompetent hosts, clinical cases of severe babesiosis have been reported from splenectomized or immunocompromised individuals. To our knowledge, only one case of human babesiosis in India has been previously reported.

A metabolically engineered spin-labeling approach for studying glycans on cells.

Metabolic glycan engineering (MGE) coupled with nitroxide spin-labeling (SL) was utilized to investigate the heterogeneous environment of cell surface glycans in select cancer and normal cells. This approach exploited the incorporation of azides into cell surface glycans followed by a click reaction with a new nitroxide spin label. Both sialic acid and -acetylglucosamine (GlcNAc) were targeted for spin labelling.

Quantification of , and genomes in commercial chicken farms by quantitative real time PCR.

Advent of quantitative polymerase chain reaction and its variants have enabled identification and quantification of seven known species of poultry in biological samples. Attempts were made in the present study to identify and quantify three important pathogenic species responsible for intestinal coccidiosis in domestic farmed chicken, and in droppings collected from thirty one poultry farms of North Indian states of Haryana, Punjab, Uttar Pradesh and Uttarakhand. The study included broiler, layer and backyard rearing units.

Mitochondrial cytochrome oxidase C subunit III (cox3) gene as a sensitive and specific target for molecular detection of Babesia gibsoni infection in dogs.

A PCR targeting mitochondrial cytochrome oxidase subunit III (cox3) for molecular detection of Babesia gibsoni infection in dogs has been developed in this study. Fifty blood samples from suspected clinical cases from dogs, brought to the veterinary college clinics, were examined for presence of B. gibsoni using conventional diagnosis by microscopic examination of Giemsa stained thin blood smears.

Canine babesiosis among working dogs of organised kennels in India: A comprehensive haematological, biochemical, clinicopathological and molecular epidemiological multiregional study.

Canine babesiosis is a serious disease among tick-borne haemoprotozoan diseases, globally. The present study was envisaged for carrying out thorough investigation of the disease among working dogs of organised kennels situated in different agro-climatic zones of India as comprehensive understanding of the disease from this country was pertinently lacking. During the study period of three years (2012-2014), 330 dogs suspected for babesiosis were examined for clinicopathology by their physical examination, haematological and biochemical parameters estimation, while the detection of apicomplexan parasites was confirmed by using various diagnostic techniques i.

Theoretical Aspects of the Cross Effect Enhancement of Nuclear Polarization under Static Dynamic Nuclear Polarization Conditions.

In this study, we perform quantum calculations of the spin dynamics of a small spin system that includes nine coupled electrons and one nucleus placed in an external magnetic field and exposed to microwave irradiation. This is an extension of a previous work in which we have demonstrated on a system of 11 coupled electron spins the dynamics of the electron polarizations composing the electron paramagnetic resonance (EPR) line during static dynamic nuclear polarization (DNP) experiments. There we have shown that the electron polarizations are determined by a spectral diffusion process, induced by the dipolar interaction and cross-relaxation.

The effects of sample conductivity on the efficacy of dynamic nuclear polarization for sensitivity enhancement in solid state NMR spectroscopy.

In recent years dynamic nuclear polarization (DNP) has greatly expanded the range of materials systems that can be studied by solid state NMR spectroscopy. To date, the majority of systems studied by DNP were insulating materials including organic and inorganic solids. However, many technologically-relevant materials used in energy conversion and storage systems are electrically conductive to some extent or are employed as composites containing conductive additives.

Experimental quantification of electron spectral-diffusion under static DNP conditions.

Dynamic Nuclear Polarization (DNP) is an efficient technique for enhancing NMR signals by utilizing the large polarization of electron spins to polarize nuclei. The mechanistic details of the polarization transfer process involve the depolarization of the electrons resulting from microwave (MW) irradiation (saturation), as well as electron-electron cross-relaxation occurring during the DNP experiment. Recently, electron-electron double resonance (ELDOR) experiments have been performed under DNP conditions to map the depolarization profile along the EPR spectrum as a consequence of spectral diffusion.

Abortions in an organized dairy farm from North India reveal the possibility of breed susceptibility to Bovine Brucellosis.

The present study was undertaken over a three year period (2012-2014) in an organized dairy farm located in North India to ascertain as the putative cause of abortion. The dairy farm maintained cattle of Frieswal, Crossbred and Sahiwal breeds and followed calf-hood vaccination with Strain 19 live vaccine in all the heifers. Even with the recommended vaccination schedule and good managemental practices in place, 88 cases of abortions clinically suspected of bovine brucellosis (40 from Frieswal breed, 17 from Crossbred cattle and 31 from Sahiwal breed) were reported from this farm.

Electron-Electron Cross-Relaxation and Spectral Diffusion during Dynamic Nuclear Polarization Experiments on Solids.

Recently it has been shown that experimental electron-electron double resonance (ELDOR) spectra of amorphous glasses containing free radicals with inhomogeneously broadened electron paramagnetic resonance (EPR) spectra can be analyzed using a set of coupled rate equations for the electron polarizations of frequency bins composing these spectra, named the eSD (electron spectral diffusion) model. The rate matrix defining these equations has elements depending on the microwave, the spin-lattice relaxation rates and on eSD rate constants responsible for polarization exchange. In this study, we show that in addition to the static dipolar flip-flop terms in the Hamiltonian a zero-quantum electron cross-relaxation mechanism can be responsible for the polarization exchange process in our samples.

Canine Monocytic Ehrlichiosis among working dogs of organised kennels in India: A comprehensive analyses of clinico-pathology, serological and molecular epidemiological approach.

Canine Monocytic Ehrlichiosis (CME) is a serious tick-borne rickettsial disease affecting canine populations globally. Besides few reports from stray and pet dogs from localised geographical regions (cities/towns/small states), a comprehensive study on prevalence of Ehrlichia canis (E. canis) among working dogs from different geo-climatic zones of India was pertinently lacking.

Humoral and cytokine response elicited during immunisation with recombinant Immune Mapped protein-1 (EtIMP-1) and oocysts of Eimeria tenella.

Eimeria tenella, the causative agent of caecal coccidiosis, is a pathogenic gut dwelling protozoan which can cause severe morbidity and mortality in farmed chickens. Immune mapped protein-1 (IMP-1) has been identified as an anticoccidial vaccine candidate; in the present study allelic polymorphism was assessed across the IMP-1 coding sequence in E. tenella isolates from four countries and compared with the UK reference Houghton strain.

Cryptic Eimeria genotypes are common across the southern but not northern hemisphere.

The phylum Apicomplexa includes parasites of medical, zoonotic and veterinary significance. Understanding the global distribution and genetic diversity of these protozoa is of fundamental importance for efficient, robust and long-lasting methods of control. Eimeria spp.

Performance and exhaust emission characteristics of variable compression ratio diesel engine fuelled with esters of crude rice bran oil.

As a substitute to petroleum-derived diesel, biodiesel has high potential as a renewable and environment friendly energy source. For petroleum importing countries the choice of feedstock for biodiesel production within the geographical region is a major influential factor. Crude rice bran oil is found to be good and viable feedstock for biodiesel production.

Population, genetic, and antigenic diversity of the apicomplexan Eimeria tenella and their relevance to vaccine development.

The phylum Apicomplexa includes serious pathogens of humans and animals. Understanding the distribution and population structure of these protozoan parasites is of fundamental importance to explain disease epidemiology and develop sustainable controls. Predicting the likely efficacy and longevity of subunit vaccines in field populations relies on knowledge of relevant preexisting antigenic diversity, population structure, the likelihood of coinfection by genetically distinct strains, and the efficiency of cross-fertilization.

Cloning and sequencing of beta-tubulin and internal transcribed spacer-2 (ITS-2) of Eimeria tenella isolate from India.

Beta-tubulin is an important multifunctional protein of eukaryotes abundant in the cytoskeleton and responsible for the formation of tubulin, structures responsible for cell morphology and which aid in motility and intracellular transportation. It has been used as a genotypic marker for studying the evolutionary history and phylogenetic relationships between eukaryotic organisms. Internal transcribed spacers of the ribosomal RNA genes have been widely used for typing inter-species and intra-species variation.

Electron spin relaxation of C60 monoanion in liquid solution: applicability of Kivelson-Orbach mechanism.

We report the results of our investigation on the electron spin relaxation mechanism of the monoanion of C60 fullerene in liquid solution. The solvent chosen was carbon disulfide, which is rather uncommon in EPR spectroscopy but proved very useful here because of its liquid state over a wide temperature range. The conditions for exclusive formation of the monoanion of C60 in CS2 were first determined using electrochemical measurements.

Electron spin-lattice relaxation mechanisms of nitroxyl radicals in ionic liquids and conventional organic liquids: temperature dependence of a thermally activated process.

During the past two decades, several studies have established a significant role played by a thermally activated process in the electron spin relaxation of nitroxyl free radicals in liquid solutions. Its role has been used to explain the spin relaxation behavior of these radicals in a wide range of viscosities and microwave frequencies. However, no temperature dependence of this process has been reported.

Development and evaluation of serodiagnostic assays with recombinant BgSA1 of Babesia gibsoni.

Indirect ELISA, dot-ELISA and double antibody sandwich ELISA (DAS-ELISA) using truncated recombinant BgSA1 (rBgSA1) were developed for detecting Babesia gibsoni infection in naturally infected dogs. Truncated BgSA1 gene of 858 bp, encoding 32 kDa protein was cloned in pET-32a(+) expression vector, expressed in Escherichia coli and the recombinant protein was purified under native conditions. To evaluate the ability of the truncated rBgSA1as serodiagnostic reagent for B.

An optimised protocol for molecular identification of Eimeria from chickens.

Molecular approaches supporting identification of Eimeria parasites infecting chickens have been available for more than 20 years, although they have largely failed to replace traditional measures such as microscopy and pathology. Limitations of microscopy-led diagnostics, including a requirement for specialist parasitological expertise and low sample throughput, are yet to be outweighed by the difficulties associated with accessing genomic DNA from environmental Eimeria samples. A key step towards the use of Eimeria species-specific PCR as a sensitive and reproducible discriminatory tool for use in the field is the production of a standardised protocol that includes sample collection and DNA template preparation, as well as primer selection from the numerous PCR assays now published.