[Biochemical bases of ftorafur-potentiating effects of perfluorodecalin, inducer of cytochrome P-450 dependent microsomal monooxygenases].

Activity of cytochrome P-450-dependent monooxygenase system is significantly lower in hepatoma H-2-73 and Lewis lung carcinoma-bearing mice as compared to that in control animals. An inhibition of the cytochrome P-450 system was observed after the injection of ftorafur. Treatment of the mice with perfluorodecalin markedly increased the antineoplastic activity of ftorafur determined by a loss of the leucocytes and of the weight hepatoma H-2-73- and Lewis lung carcinoma resistant to ftorafur alone.

[The effect of ziksorin on the development of experimental food anaphylaxis in guinea pigs].

A study was made of the effect of zixorin, an inductor of liver cytochrome P-450, on the intensity of the immediate type allergic reaction--active food anaphylaxis in response to ovalbumin in guinea-pigs. Intraperitoneal injection of zixorin in a dose of 50 mg/kh for 3 days before administration of the antigen resolution dose was found to lower 2,3-fold the level of lethal outcomes due to the anaphylactic shock. It is suggested that zixorin may be used in multimodality treatment of food allergy.

[Effects of zixoryn on pharmacokinetics of antipyrine in intact and sensitized guinea pigs].

The effect of zixoryn, a hepatic inductor of cytochrome P-450, on the pharmacokinetics of antipyrine in intact and sensibilized guinea-pigs was studied. It was found that sensibilization of albumin increased the half-time (T1/2), AUC and decreased the total body clearance (Clt) of antipyrine and the renal clearance (Clr) of metabolites in urine. The administration of zixoryn in sensibilized animals decreased T1/2 and AUC and increased the clearance of antipyrine and its metabolites.

[The effect of the adamantylamides of 1,3-diphenylpyrazole-4-carboxylic acids on the cytochrome P-450 system of the liver].

The effect of adamanthylamides of 1,3-diphenylptrazol-4-carbonic acids on the system of the liver microsomal monoxygenases was studied. A potent induction of the liver cytochrome P-450 by 1,3-diphenylpyrazol-5-methyl-4-carbonic acid, N-methyl-N-(adamant-1-il)amide was found. As a consequence of the induction of the liver cytochrome P-450, the compound reduced by 40% the degree of the delayed type hypersensitivity in mice.

[Post-stress correlation of the functional activity of macrophages by tuftsin and its derivatives].

The effect of 15 day programmed neurotization on the functional activity of the peritoneal macrophages has been studied. The NBT-test and the adhesion measurements were used. The experimental neurosis resulted in the decrease of the macrophage functional activity and in leukopenia.

[Effect of the induction of liver cytochrome P-450 by a chemically inert fluorocarbon on the reactions of immediate and delayed hypersensitivity].

The effect of chemically inert perfluorodecalin (PFD) on the development of hypersensitive reactions of immediate and delayed types in guinea pigs and mice was studied. It was found that administration of PFD to animals both before sensitization (mice) and during the development of sensitization (guinea pigs) reduced the degree of the both types of hypersensitivity.

[Relation between the toxic killing of mouse small intestine enterocytes and the interval between the administration of the S-specific agent hydroxyurea].

The dependence of small intestinal lesions on the interval between repeated regular injections of hydroxyurea was studied. The doses of 1 g/kg and 0.2 g/kg were used.

[Relation of the survival of mouse hematopoietic stem cells to the interval between periodic injections of hydroxyurea].

The dependence was studied between the survival of proliferating hemopoietic stem cells (CFUs) on the interval between regular multiple injections of hydroxyurea (HU). Before HU injection mice were irradiated in a dose of 200 rad to trigger the proliferation of CFUs. The dependence obtained is resonance in character, with a maximum being attained during injections made with a 12-hour interval.

[Mechanism of the effect of immobilized noradrenaline on the contact phase of blood coagulation].

A deficiency in the contact phase of blood coagulation, depending on the specific isolation of Fletcher's factor (prekallikreins) from blood plasma, was observed after chromatography of blood plasma on a column with noradrenaline-Sepharose. Activation of kalliklein and dekinination of high-molecular kininogene occurred after the interaction between proenzymes of contact phase of blood coagulation (factor XII, prekallikreins, high-molecular kininogene).

[Kinin system components, free kinins and proteinase inhibitors in the edematous fluids of nephrotic syndrome patients].

Main components of the kinin system, free kinins, total arginine esterase activity content of alpha 1-antitrypsin and alpha 2-macroglobulin fractions were estimated in various edematous fluids (transduates of different localization, pleural exudates of the inflammatory type) of patients with nephrotic syndrome of various etiology. Noninflammatory edematous fluids (interstitial, abdominal and pleural transudates) were found to contain activated kallikrein and prekallikrein from blood plasma; 3-10 ng/ml of free kinins were present in interstitial edematous fluids and 30-60 ng/ml - in abdominal transudate. Kinins of abdominal transudate were identified with bradikinin by chromatographic properties; a single low-molecular form of kininogene was found, its content did not exceed 10% of the substance occurring in blood plasma of the patients.

[Effect of a polyvalent proteinase inhibitor from the lungs of a bull on the state of the kinin system in burn shock and acute burn toxemia].

Effect of a polyvalent inhibitor of proteinases from bovine lungs (industrial preparation -- ingitrile, commercial grade -- conntrical) on the state of kinin system was studied in blood serum of patients with extensive burns at shock periods and in acute burn toxemia. Decrease in content of kallikreinogene, which is typical for shock and acute, toxemia and which reflects the activation of kallikrein-kinin system, was less distinct in patients, treated with the inhibitor, than in patients, which were not treated with ingitrile. The early and rapid restoration in kininogene content and distinct inhibition of the total arginine-esterase activity were observed in blood serum after treatment with the inhibitor.

[State of the kinin system and level of serum proteinase inhibitors in latent nephritis and the nephrotic syndrome of different etiology].

Main components of kinin system, the arginine-esterase activity and proteinase inhibitors were estimated in blood serum of patients with nephrotic syndrome of various etiology (glomerulonephritis, amyloidosis, systemic lupus erythematous) and also in patients with latent nephritis and in healthy donors. Content of all the kinin system components (kallikreinogen, kininogen and kininase 1) proved to be increased in all the forms of nephropathy studied. Free kallikrein was found in blood serum of patients with nephrotic syndrome as distinct from healthy persons and patients with latent nephritis.

[Effect of trypsin inhibitor of a peptide-protein nature on kallikreins from human and rabbit blood stream].

The effect of three natural trypsin inhibitors--polyvalent Kunitz inhibitor (BPTI), the inhibitor from cow colostrum (CTI) and the inhibitor from soybean (SBTI)--on esterase and kininogenase action of partially purified kallikrein preparations from human and rabbit blood serum is studied. The effect of each inhibitor was estimated from Ki values. The latters show that BPTI, SBTI and CTI are strong inhibitors of both kallikreins.