Multi-Disciplinary Monitoring Networks for Mesoscale Underground Experiments: Advances in the Bedretto Reservoir Project.

The Bedretto Underground Laboratory for Geosciences and Geoenergies (BULGG) allows the implementation of hectometer (>100 m) scale in situ experiments to study ambitious research questions. The first experiment on hectometer scale is the Bedretto Reservoir Project (BRP), which studies geothermal exploration. Compared with decameter scale experiments, the financial and organizational costs are significantly increased in hectometer scale experiments and the implementation of high-resolution monitoring comes with considerable risks.

In situ observation of helium and argon release during fluid-pressure-triggered rock deformation.

Temporal changes in groundwater chemistry can reveal information about the evolution of flow path connectivity during crustal deformation. Here, we report transient helium and argon concentration anomalies monitored during a series of hydraulic reservoir stimulation experiments measured with an in situ gas equilibrium membrane inlet mass spectrometer. Geodetic and seismic analyses revealed that the applied stimulation treatments led to the formation of new fractures (hydraulic fracturing) and the reactivation of natural fractures (hydraulic shearing), both of which remobilized (He, Ar)-enriched fluids trapped in the rock mass.

Comprehensive geological dataset describing a crystalline rock mass for hydraulic stimulation experiments.

High-resolution 3D geological models are crucial for underground development projects and corresponding numerical simulations with applications in e.g., tunneling, hydrocarbon exploration, geothermal exploitation and mining.

Isolation of phosphoglycerate kinases by affinity chromatography.

A variety of Sepharose derivatives containing DL-O-phosphorylserine or adenosine nucleotides with different points of attachment, has been synthesized and tested for affinity to phosphoglycerate kinase. The most effective gels contained periodate-oxidized ATP or ADP bound via the ribose by hydrazone formation to adipoyl-dihydrazo-Sepharose. The effect of pH, magnesium and buffer ions on the binding capacity of the ATP derivative of Sepharose has been examined.

Hereditary deficiency of phosphoglycerate kinase: a new variant in erythrocytes and leucocytes, not associated with haemolytic anaemia.

An X-chromosome linked phosphoglycerate kinase deficiency in erythrocytes and leucocytes was discovered in a large German kindred. Seven males of two generations were found to have only 21% of the normal enzyme activity in their erythrocytes, and twelve females of three generations showed various degrees of this defect. The differences in the expression of the deficiency in heterozygote females are explained by the Lyon hypothesis.