Solubilization of brain mitochondrial hexokinase by thiopental.

The control of hexokinase activity probably is accomplished by regulating the partitioning of the enzyme between soluble and particulate forms, the latter being more active. In the present investigation we have examined the thiopental effect on the cerebral hexokinase distribution. In anesthesia, after administration of thiopental to male Sprague Dawley rats, the increase of the soluble fraction of hexokinase was dose dependent.

Decreased GABA and glutamate concentration in rat brain after treatment with 6-aminonicotinamide.

The effect of 6-aminonicotinamide (6-AN) on putative amino acid neurotransmitters, namely glutamate, GABA and aspartate was studied on brains of rats treated with this antimetabolite (35 mg/kg i.p.).

[Influence of diazepam and phenytoin on penicillin-induced cerebral convulsions (author's transl)].

The effects of diazepam and phenytoin on penicillin-induced EEG changes and convulsions were studied in 32 rabbits. EEG changes and convulsions were elicited by 2.4 g/kg benzylpenicillin i.

Decreased glycolytic flux rate in the isolated perfused rat brain after pretreatment with 6-aminonicotinamide.

Cerebral energy metabolism was studied in the isolated perfused rat brain after 6-aminonicotinamide (6-AN; 35 mg/kg i.p.) administered to the intact animals 7 hrs before perfusion was started.

The effects of increased glucose supply and thiopental anesthesia on energy metabolism of the isolated perfused rat brain.

The effects of glucose concentrations in the perfusion medium ranging from 5 to 15 mM and thiopental, on cerebral energy metabolism were studied using the isolated perfused rat brain. After a perfusion time of 30 min brain levels of the following substrates and metabolites were determined: P-creatine, ATP, ADP, AMP, glycogen, glucose, glucose-6P, fructose-6-P, pyruvate, lactate, alpha-ketoglutarate, glutamate, ammonia. In control experiments increasing the glucose concentration in the perfusion medium produced an increase of intracellular brain glucose concentration only, revealing a linear relationship between glucose content in brain and blood.

[Bacterial growth in the perfusion medium of the isolated rat brain and its inhibition by antibiotics].

In the perfusion medium of the isolated rat brain the bacterial content was examined. The perfusion medium used contained 30% bovine erythrocytes, 2 g% bovine serum albumin and about 13 mM glucose in Krebs-Henseleit-solution. Before the perfusion of the isolated rat brain was started the medium recirculated up to two hours in the perfusion apparatus.