A study of macroinvertebrate communities in Bolshiye Koty Bay of Lake Baikal using DNA metabarcoding.

The diversity of macroinvertebrates, the structure of their communities in Bolshiye Koty Bay (Lake Baikal) was studied by a DNA metabarcoding approach using an Illumina MiSeq system. Internal primer mlCOIintF in combination with jgHCO2198 of the Folmer fragment of the COI gene were used for macroinvertebrate metabarcoding. A total of 118009 reads of the COI gene fragment (at least 313 bp in length) were obtained.

DNA metabarcoding of benthic algae and associated eukaryotes from Lake Baikal in the face of rapid environmental changes.

Here we report new data describing the biodiversity of phytobenthic communities based on DNA-metabarcoding using the 18S rDNA marker and the Illumina MiSeq system. The study was initiated due to the blooming of f ilamentous algae (mainly of the genus Spirogyra) and cyanobacteria in the coastal zone of Lake Baikal under climate change and anthropogenic impact. The composition and taxonomic diversity of algae and other organisms associated with them on different sites of Lake Baikal (near Bolshoi Ushkaniy Island, in Listvennichny Bay) and in the Kaya (within the city of Irkutsk, located in the same drainage basin as Lake Baikal) were determined using DNAmetabarcoding.

Response of Aquatic Organisms Communities to Global Climate Changes and Anthropogenic Impact: Evidence from Listvennichny Bay of Lake Baikal.

Recent studies have revealed how the freshwater biota of Lake Baikal responds to climate change and anthropogenic impacts. We studied phyto- and zooplankton, as well as phyto- and zoobenthos, in the open coastal waters of the southern basin of the lake and of Listvennichny Bay. A total of 180 aquatic organism taxa were recorded.

Complex Bioactive Supplements for Aquaculture-Evolutionary Development of Probiotic Concepts.

The use of novel and effective probiotic-based immunostimulating preparations, prebiotics, metabiotics, and phytobiotics is considered as a promising direction for the creation of new complex feed additives for aquaculture enterprises to increase the health and productivity of the cultivated hydrobionts. The information on the products of anaerobic solid-phase fermentation of plant substrates is presented as the basis for new probiotic-based additives for aquacultures. Biologically active feed additives ProStor and GerbaStor containing probiotics and medicinal herbs and their effects on aquaculture are discussed.

[The disability due to industrial injuries and occupational diseases in the Arkhangelsk Oblast].

The article presents analysis of primary disability in the Northern Region as compared with The Russian Federation. The dynamics and structure of disability due to occupational injuries and diseases is presented. In 2008-2018, in the Arkhangelsk Oblast the level of disability for the analyzed group of causes decreased up to 9.

Metavirome datasets from two endemic Baikal sponges .

Sponges are ecologically important components of marine and freshwater benthic environments; these holobionts contain a variety of microorganisms and viruses. For the metagenomic characterization of potential taxonomic and functional diversity of sponge-associated dsDNA viruses, we surveyed two samples of Baikal endemic sponge (diseased and visually healthy). In total, after quality processing, we have obtained 3 375 063 and 4 063 311 reads; of these 97 557 and 88 517 sequences, accounting for ca.

The diversity of the Baikal lineage of Pallas, 1766: molecular and morphological evidence.

In this paper, molecular analyses of Baikal hydras from the ' group', based on COI and ITS1-5.8S-ITS2, and morphological analysis of their holotrichous isorhizas, were performed. Low genetic diversity and shared haplotypes were found between Pallas, 1766 and Swarczewsky, 1923 specimens, which is evidence of the mixing of these lineages.

The effect of bloom of filamentous green algae on the reproduction of yellowfin sculpin Cottocomephorus grewingkii (Dybowski, 1874) (Cottoidae) during ecological crisis in Lake Baikal.

In shallow water areas of open Lake Baikal, filamentous green alga of the genus Spirogyra grows abundantly. Together with alga of the genus Ulothrix, it forms algal mats. According to our observations from 2010 to 2013, the spawning habitat conditions for the yellowfin sculpin Cottocomephorus grewingkii (Dybowski, 1874) (Cottidae) proved to be significantly disturbed in the littoral zone of Listvennichnyi Bay (southern Baikal), which, in turn, reduced the number of egg layings.

[Genetic Differentiation of Populations of Baikal Endemic Sergentia baicalensis Tshern. (Diptera, Chironomidae)].

The population structure of endemic species Sergentia baicalensis (Diptera, Chironomidae) from Lake Baikal was studied using the first subunit of the cytochrome C oxidase mitochondrial gene (Col). Two populations inhabiting different basins of this lake, the southern-central and northern, were detected. It was confirmed that the divergence time of this species was dated to Late Miocene (9.

[Nuclear-cytoplasmic compatibility and the state of mitochondrial and chloroplast DNA regions in alloplasmic recombinant and introgressive lines (H. vulgare)-T. aestivum].

Alloplasmic lines combining alien nuclear and cytoplasmic genomes are convenient models for studying the mechanisms of nuclear-cytoplasmic compatibility/incompatibility. In the.present study, we have investigated the correlation between the characters and state of mitochondrial (mt) and chloroplast (cp) DNA regions in alloplasmic recombinant common wheat lines with barley cytoplasm characterized by partial or total fertility.

[Evolutional relationships of endemic green algae Draparnaldioides simplex from Lake Baikal with nonbaicalian taxa of family Chaetoforaceae (Chlorophyta)].

Phylogenetic relationships between the endemic baicalian green algae Draparnaldioides simplex C. meyer et Skabitsch, 1976 and holarctic taxa of green algae were studied using the fragment of 18S rDNA and internal transcribed spacers ITS1 and ITS2 of nuclear DNA. We showed that the baicalian genus Draparnaldioides is a separate taxon.

[Specific features of fertility restoration in alloplasmic lines obtained based on hybridization of self-fertilized offspring of barley-wheat (Hordeum vulgare L. x Triticum aestivum L.) amphiploid with common wheat varieties Saratovskaya 29 and Pyrotrix 28].

The problems of fertility restoration in the progeny of barley-wheat hybrids (H. vulgare x T. aestivum) are explained by incompatibility between the cytoplasm of cultivated barley and the nuclear genome of common wheat.

[Characteristics of common wheat cultivars of West Siberia carrying the wheat-rye 1RS.1BL translocation].

Using genomic in situ hybridization, among the common wheat cultivars produced in West Siberia (Siberian Research Institute of Agriculture, Omsk) with the involvement of the winter wheat cultivar Kavkaz carrying the wheat-rye 1RS.1BL translocation we identified three cultivars with this translocation: Omskaya 29, Omskaya 37, and Omskaya 38. The protein and crude gluten contents in the grain of these cultivars are equal to or exceed the levels observed in cultivars without the wheat-rye translocation.

[Zoobenthos communities and their seasonal dynamics in nonfreezing springs of Baikal region].

The faunal composition, structure of the zoobenthos communities, and seasonal changes in two springs in the south of Irkutsk area are investigated. Both springs have the water temperature 4-5 degrees C all year round. The fauna consists of widely distributed hydrobionts, crenobionts, stygobionts, and near-water organisms.

[Production of alloplasmic and euplasmic wheat-barley ditelosomic substitution lines 7H(1)Lmar(7D) and analysis of the 18S/5S mitochondrial repeat in these lines].

Alloplasmic lines of common wheat with disomic substitution of chromosome 7D for telocentric chromosome 7H(1)Lmar of barley H. marinum subsp. gussoneanum Hudson were isolated from the plants of generation BC3, produced as a result of backcrossing of barley-wheat hybrids H.

[Features of alloplasmic wheat-barley substitution and addition lines (Hordeum marinum subsp. gussoneanum)-triticum aestivum].

Two alloplasmic wheat-barley substitution lines were studied: a line replaced at three pairs of chromosomes 1Hmr((IB), 5Hmar(5D), and 7Hmar(7D), and the disomic-substituted line 7Hma(7D). The lines were constructed on the basis of individual plants from BCIF8- and BC2F6 progeny of barley-wheat hybrids (H. marinum subsp.

[Production of wheat-rye substitution lines based on winter rye cultivars with karyotype identification by means of C-banding, GISH, and SSR markers].

The study presents a continuation of the research aimed at producing of wheat-rye substitution lines based on the cross (Triticum aestivum L. x Secale sereale L.) x Triticum aestivum L.

[Effect of rye Secale cereale L. chromosomes 1R and 3R on polyembryony expression in hybrid combinations between (Hordeum vulgare L.)-Triticum aestivum L. alloplasmic recombinant lines and wheat T. aestivum L.-rye S. cereale L. substitution lines].

The effect of rye chromosomes on polyembryony was studied for reciprocal hybrid combinations between (Hordeum vulgare L.)-Triticum aestivum L. alloplasmic recombinant lines and five wheat T.

[Features of the formation of self-fertile euploid lines (2n = 42) by self-pollination of the 46-chromosome barley-wheat BC1 hybrid Hordeum marinum subsp. gussoneanum Hudson (= H. geniculatum All.) (2n = 28) x Triticum aestivum L. (2n = 42)].

We studied some features of the development of self-fertile 42-chromosome lines on the base of self-pollination progeny of 46-chromosome plants obtained by backcrossing of barley--wheat hybrids Hordeum marinum subsp. gussoneanum Hudson (= H. geniculatum All.

[Production of wheat-rye substitution lines and identification of chromosome composition of karyotypes using C-banding, GISH, and SSR markers].

Based on the cross (Triticum aestivum L. x Secale cereale L.) x T.

[Features of crossability, haploidy and polyembryony in hybrid combinations between common barley Hordeum vulgare L. (2n = 14) and wheat-rye substitution lines Triticum aestivum L., cultivar Saratovskaya 29/Secale cereale L., cultivar Onokhoiskaya].

The role of individual chromosomes of rye in the manifestation of crossability and seedling development in hybrid combinations between common barley Hordeum vulgare L., cultivar Nepolegayushchii (2n = 14) and five wheat-rye substitution lines Triticum aestivum L., cultivar Saratovskaya 29/Secale cereale L.

[Mechanisms of meiotic restitution and their genetic regulation in wheat-rye polyhaploids].

Meiosis has been studied in partially fertile wheat-rye F1 hybrids yielded by crosses Triticum aestivum (Saratovskaya 29 variety) x Secale cereale L. (Onokhoiskaya variety) (4x = 28). Hybrid self-fertility proved to be caused by formation of restituted nuclei, which appear after equational segregation of univalent chromosome in AI and sister chromatid non-separation in AII of meiosis, as well as after AI blockage in three different ways.

[The effect of rye chromosomes on callus induction and regeneration in callus cultures of immature embryos of wheat-rye substitution lines, Triticum aestivum L. cultivar Saratovskaia 29/Secale cereale L. cultivar Onokhoiskaia].

The effect of individual rye chromosomes on the induction of callus and the character of its regenerating capacity was studied with cultured immature embryos of wheat-rye (Triticum aestivum L. cv. Saratovskaya 29-Secale cereale L.