Increasing the efficiency of hypolipidemic therapy with the combined use of quercetin in patients with non-alcoholic fatty liver disease on the background of the metabolic syndrome.

The article analyzes the results of a set of biochemical indicators in the course of treatment with the combined use of rosuvastatin with quercetin in patients with metabolic syndrome (MS) combined with non-alcoholic fatty liver disease. Changes in blood biochemical parameters have been identified and presented with MS, essential for revealing generalbiological mechanisms development and interrelationship between the components of MS and non-alcoholic fatty liver. The effect of an increase in free cholesterol and triglycerides and activation of freeradical oxidation of lipids followed by theaccumulation of oxidative stress products was noted.

Seasonal variation of immune response to heterologous erythrocytes in natural populations of red-backed () and gray-sided () voles in Western Siberia.

We studied the seasonal variation of adaptive humoral immunity (AHI) in northern red-backed vole ( Pallas, 1779, RBV) and gray-sided vole ( Sundevall, 1846, GSV) in Tomsk region of Western Siberia. Immunoresponsiveness (IR) to sheep red blood cells was assessed by the number of antibody-producing cells in the spleen. The use of a generalized linear model to analyze the effects of species, sex, year of research, and season of withdrawal of individuals from nature on IR showed a significant effect of species identity, season of animal capture, and the interaction of species with season.

DETERMINATION OF SAFETY PROFILE OF REPARATIVE ACTIVITY OF THE NEW HYGIENIC PRODUCT "APIPROL".

Objective: The aim: To prove safety of the new elixir and determine the effect on regenerative processes under conditions of the experimental trauma.

Patients And Methods: Materials and methods: The formula of the new dental elixir "Apiprol" includes biologically active components of bee products (propolis, comb capping wax), plant origin compounds. The elixir was single administered, intragastrally to white nonlinear mice weighing (22±2) g at doses at 50 mg/kg, 500 mg/kg, 5000 mg/kg.

Lipoic Acid Exacerbates Oxidative Stress and Lipid Accumulation in the Liver of Wistar Rats Fed a Hypercaloric Choline-Deficient Diet.

Non-alcoholic fatty liver disease (NAFLD) is currently estimated as the most prevalent chronic liver disease in all age groups. An increasing body of evidence obtained in experimental and clinical data indicates that oxidative stress is the most important pathogenic factor in the development of NAFLD. The study aimed to investigate the impact of α-lipoic acid (LA), widely used as an antioxidant, on the effects of a hypercaloric choline-deficient diet.

Detrimental effects of 6 months exposure to very low doses of a mixture of six pesticides associated with chronic vitamin deficiency on rats.

This study aimed to evaluate the long-term low-dose effects of exposure to a mixture of 6 pesticide active substances (diquat, imazamox, imazethapyr, tepraloxydin, bentazone, acifluorfen) and to elucidate if chronic vitamin deficiency can influence their toxicity. Two hundred Wistar rats were divided in 4 groups: a vitamin-sufficiency control group, a vitamin-deficiency control group, a vitamin sufficiency test group and a vitamin-deficiency test group. The test groups were treated with the aforementioned pesticides at doses 100 times lower than the corresponding NOAEL.

Imbalance of acquired immunity factors in newborns with cytomegaloviral infection depending on the severity of the disease.

Objective: to assess the imbalance of the parameters of the immune status at the time of manifestation of CMVI in newborns in order to predict the development of a severe form of the disease.

Methods: 133 newborns with CMVI were examined. Depending on the severity of the condition, all children were divided into two groups: 1 - CMVI, severe form - 60 people (45.

[Nutriome as the direction of the "main blow": determination of physiological needs in macro- and micronutrients, minor biologically active substances].

One of the essential parts of fundamental research in Nutrition Science is the determination of the physiological requirements of humans for energy and food substances. Research that has been carried out in this area over the past 90 years, consistently develops and improves the norms of physiological requirements for energy and nutrients for various groups of the population of the Russian Federation. In the 50 years of the last century in this research field, determining the values of daily intake for macronutrients (proteins, lipids and carbohydrates), was in the first place.

Local Use of Apisan Gel, A New Oral Care Product in the Treatment of Experimental Periodontitis Against the Background of Hyperacid Gastritis and Intoxication with Tobacco Smoke.

Objectives: The aim of the work was to substantiate the use of a newly created oral care product in the treatment of periodontal disease reconstructed against the background of hyperacidic gastritis under the conditions of tobacco smoke intoxication.

Materials And Methods: The study was conducted in 2 stages. In the first stage, all experimental animals were divided into 4 groups: 1) intact, 2) with simulated periodontitis, 3) with reproduced periodontitis against the background of reproduced hyperacidic gastritis, 4) with reproduced periodontitis against the background of hyperacid gastritis under the conditions of tobacco smoking.

[Effects of polyphenols on activity of glycosyl hydrolases in the cecum of rats fed obesity inducing diets].

The results of experimental studies indicate that the preventive and therapeutic effects of polyphenols in obesity are accompanied by a significant decrease in the severity of dysbiosis caused by the predominance of fats and simple carbohydrates in the diet, especially fructose, and the restoration of the functional state of the microbiota. of the work was to study the effect of quercetin and resveratrol - polyphenols, widely represented in the daily human diet, on the activity of bacterial glycosidases in rats receiving diets high in fructose or fat and fructose. .

Efficiency of local therapy with the use of a new preparation for oral care at periodontitis in the course of hyperpeptic gastritis after tobacco smoke intoxication.

Objective: Introduction: Periodontal diseases, arising against a background of stomach pathology at tobacco addiction remain an acute problem of modern dentistry. The aim of the work is an experimental study of efficiency of application of the new preparation for oral care during treatment of periodontitis simulated against a background of hyperpeptic gastritis under conditions of intoxication by tobacco smoke.

Patients And Methods: Materials and methods: At the first stage all experimental animals were divided into 4 groups: І - intact, II - with simulated periodontitis, III - with simulated periodontitis against a background of simulated hyperpeptic gastritis, IV - with simulated periodontitis against a background of hyperpeptic gastritis under conditions of tobacco smoking.

[The role of polymorphism of the interferon gene ɣ and interferonoproduction in the pathogenesis of infection caused by herpes 6 type virus in children of early age.].

The purpose of the article is to reveal the immunological features of the infection caused by the herpesvirus type 6 virus, based on the level of the polymorphism IFNG geneγ (+874 A / T) and interferon production. Group I (n = 58) consisted of children with herpetic infection of type 6, severe form. Group II (n = 31) included children with herpetic infection of type 6, medium-heavy form.

[Effects of quercetin on protective capacity in rats fed a high-fructose diet].

The purpose of the study was to determine effects of quercetin on protective capacity parameters in the experiment on rats fed a high fructose diet. Rats of the control group received a semi-synthetic (s/s) diet and water; animals from the 1st experimental group - s/s diet and 20% fructose solution instead of drinking water; rats of the 2nd experimental group- s/s diet with quercetin (0.1% indiet) and 20% fructose solution instead of drinking water for 20 weeks.

INFLUENCE OF OZONE THERAPY ON ORAL TISSUE IN MODELING OF CHRONIC RECURRENT APHTHOUS STOMATITIS.

Chronic recurrent aphthous stomatitis (CRAS) belongs to the group of chronic, inflammatory, ulcerative diseases of the oral mucosa. The aim of this study was to determine the effects of ozone on the morphofunctional peculiarities of the soft tissues in modeling chronic recurrent aphthous stomatitis. We performed experimental investigation for study of the morpho-functional state of tissues of the oral mucosa in CRAS with using of previously proposed and widely used modeling scheme with ovalbumin and aluminum hydroxide.

[The influence of separate and combined supplementation with curcumin and quercetin on the protective capacity in rats].

The purpose of the study was to determine the effects of curcumin (CUR) and quercetin (QUER) on the expression of genes and activity of prototypical Nrf2/ARE- and AhR/ XRE-regulated enzymes. Investigation was carried out on male Wistar rats with initial body weight (230-235 g b.w.

[Toxicological evaluation of colloidal nano-sized silver stabilized polyvinylpyrrolidone. III. Enzymological, biochemical markers, state of antioxidant defense system].

Nanosized colloidal silver (NCS) with primary nanoparticles (NPs) size in the range of 10-80 nm in aqueous suspension was administered to rats with initial weight 80±10 gfor the first 30 day intragastrically and for lasting 62 days with the diet consumed in doses of 0.1; 1.0 and 10 mg/kg of body weight b.

[Effects of rutin on protective capacity in rats].

The purpose of the study was to determine effects of rutin dietary administration on the activity of xenobiotic-metabolizing enzymes and antioxidant status. The study has been carried out on 3 groups of male Wistar rats (n = 8 in each), with initial body weight 100-120 g. Animals of the control group (1st group) received standard semi-synthetic diet, the experimental groups--the same diet with rutin in the amount of 40 mg/kg b.

[Effect of rutin and hesperidin on the expression of gene and the activity of hemoxygenase-1 and NAD(P)H-quinone oxidoreductase at their separate and combined action].

Flavonoids rutin (R) and hesperidin (Hes) have a broad spectrum of the biological activity based on their antiradical properties and ability to increase the activity of antioxidant enzymes, including the activity of heme oxygenase-1 (HO-1) and NAD(P)H-quinone oxidoreductase (QR). It is supposed that the main regulator of the activity of HO-1 and QR is the transcription factor Nrf2. The purpose of the study was to determine the effects of R and Hes on the expression of Nrf2 gene and protein, on the activity and mRNA and protein expression of HO-1 and QR at their separate and combined action.

[The effect of rutin and hesperidin on the expression of Nrf2- and AhR-regulated genes and CYP3A1 gene in rats intoxicated with carbon tetrachloride].

The purpose of the study was to determine the effects of rutin (R) and hesperidin (Hes), the main representatives of two most studied subclasses of flavonoids - flavonols and flavanones, on the expression of prototypical Nrf2 and AhR-regulated genes and CYP3A1 gene in rats intoxicated with carbon tetrachloride (CCl4). Investigations were carried out on 5 groups of male Wistar rats with the initial body weight (b.w.

[Effect of minor bioactive food substances – rutin and hesperidin in their separate and combined alimentary arrives on the immune system of rats and the activity of nuclear factor NF-kB liver cells].

The effect of rutin and hesperidin in their separate and combined admission to the immune system and the activity of nuclear factor NF-kB of rat liver cells has been investigated. Wistar male rats with an initial body weight of 224–225 g were divided into 4 groups of 6 rats in each. The rats of the 1st group (control) received a complete semi-synthetic diet, rats in group 2 – the same diet supplemented with rutine (400 mg/kg b.

[Toxicological assessment of nanostructured silica. II. Enzymatic, biochemical indices, state of antioxidative defence].

Nanostructured silica (SiO2) "Aerosil" with the size of the primary nanoparticles (NPs) of 5-30 nm, in the form of ultrasound treated water suspension was administered to rats of 80 ± 4 g initial body weight for the first 30 days by intragastric gavage and then for 62 days with diets consumed in doses of 0.1; 1.0; 10 and 100 mg/kg body weight per day.

[Effects of vitamins deficiency on the cytochrome P450 inducibility in rats].

The purpose of the study was to determine multi-vitamin deficiency effects on the inducibility of main isoforms of cytochrome P450 in the rat liver. The study was carried out on 4 groups of Wistar rats. Rats of the 1st and 3rd group received semi-synthetic diets containing adequate (100% of recommended vitamin level) level of vitamins, the 2nd and 4th--the semi-synthetic diet containing vitamins in the amount of 20% from adequate level.

Effect of polyunsaturated fatty acids ω-3 on the induction of activity and expression of CYP1A1 and CYP1A2 genes in the liver of rats under the influence of indole-3-carbinol.

Supplementation of the ration with eicosapentaenoic and docosahexaenoic ω-3 polyunsaturated fatty acids (PUFA) in doses of 0.3 and 1 g/kg body weight for 4 weeks had no effect on ethoxyresorufin O-dealkylase (EROD) activity and expression of the CYP1A1 gene in male Wistar rats, but caused a dose-dependent increase in methoxyresorufin O-dealkylase (MROD) activity of CYP1A2 (by 28 and 73%, respectively) without significant changes in CYP1A2 mRNA expression. ω-3 PUFA had no effect on the indole-3-carbinol-induced (20 mg/kg body weight over the last 7 days of the experiment) EROD activity and expression of CYP1A1 mRNA.

[Effects of rutin on the activity of antioxidant enzymes and xenobiotic-metabolizing enzymes in liver of rats fed diets with different level of fat].

The study has been carried out on 6 groups of male Wistar rats, which received semi-synthetic diets within 28 days. Rats of 1st and 4th group received fat-free diet, 2nid.and 5th - diet containing standard amount of fat (10% by weight, 26% by caloric content; lard/sunflower oil - 1/1); 3rd and 6th group - a high-fat diet (30% by weight, 56% by caloric content).

Activity of xenobiotic-metabolizing enzymes in the liver of rats with multi-vitamin deficiency.

The purpose of the study was to determine how multi-vitamin deficiency affects xenobiotic-metabolizing enzyme (XME) activities in the rat liver. Vitamin levels and XME activities were studied in the livers of male Wistar rats who were fed for 4 weeks with semi-synthetic diets containing either adequate (100 % of recommended vitamin intake) levels of vitamins (control), or decreased vitamin levels (50 % or 20 % of recommended vitamin intake). The study results have shown that moderate vitamin deficiency (50 %) leads to a decrease of vitamin A levels only, and to a slight increase, as compared with the control, in the following enzyme activities: methoxyresorufin O-dealkylase (MROD) activity of CYP1 A2 - by 34 % (p < 0.