[Influence of propagating electrical signals on delayed luminescence of geranium leaves. II. Theoretical analysis].

A mathematical model of delayed luminescence was devised. It is based on the description of primary photosynthetic reactions, which takes the donor and acceptor parties of photosystem II and the electrochemical gradient on thylakoid membranes into account. The model was used for the theoretical analysis of the mechanisms of changes in delayed luminescence characteristics, induced by variation potentials.

[Influence of propagating electrical signals on delayed fluorescence of geranium leaves. 1. Experimental analysis].

It was shown that local damaging stimulation of geranium graft induced the generation and propagation of electrical signals (variation potential), which changed the parameters of delayed luminescence in the leaf blade. The responses of delayed luminescence included two stages, which probably occur by different mechanisms.

[Electrophoretic analysis (SDS PAGE) of ovulatory cervical mucus in patients with fertility failure and after unsuccessful IVF].

Objective: To evaluate the presence and activity of local sperm antibodies and electrophoretic analysis of ovulatory cervical mucus (OCM) to prove the correlation of results of Kremer mucus sperm capillary penetration test and i-MAR (mixed antiimmunoglobulin reaction) test with SDS-PAGE.

Design: Retrospective study.

Setting: Department of Obstetrics and Gynecology, Medical Faculty of Charles University, Pilsen.

Anti-phospholipid antibodies against phosphatidylinositol, and phosphatidylserine are more significant in reproductive failure than antibodies against cardiolipin only.

Problem: The humoral immune response to phospholipids was investigated in women with reproductive failure [1073 women after one in vitro fertilization (IVF), 853 women after two and more IVF, 627 women after three and more repeated spontaneous miscarriages or missed abortions, 412 women after diagnostic laparoscopy] and compared with that of 391 healthy fertile women.

Method Of Study: Sera from all women in the study were tested by enzyme-linked immunosorbent assay (ELISA) for the detection of IgG, IgA, and IgM isotypes of antibodies against seven phospholipids (aPLs), i.e.

Six kinds of anti-phospholipid antibodies (aPLs) in ovulatory mucus and seminal plasma from couples with repeated miscarriages.

Objective: Determination of aPLs in ovulatory mucus and in seminal plasma in 18 infertile couples (women, median age 33.2, range 28-39 years; men, median age 36.4, range 29-46 years) with repeated unexplained miscarriages (four to nine spontaneous abortions).

Immunity to spermatozoa and male fertility.

Immunological characterization of semen and male fertility are discussed. The pH of seminal plasma is very similar in groups of fertile and infertile men. Nonspecific immunological factors such as lysozyme, C3c, alpha-1 antitrypsin and beta-2 microglobulin were significantly higher in 14% of infertile men.

Studies on associations between human leukocyte antigen (HLA) class II alleles and antiphospholipid antibodies in Danish and Czech women with recurrent miscarriages.

Autoantibody production is commonly associated with particular HLA class II phenotypes. The aim of the present study was to investigate whether the presence of antiphospholipid (APL) antibodies and other autoantibodies in women with unexplained recurrent miscarriage was associated with particular human leukocyte antigen (HLA)-DR or -DQ alleles or linked epitopes which have previously been reported as being associated with the recurrent miscarriage syndrome or the presence of APL. In a total of 123 Danish and Czech women with recurrent miscarriage, serum was investigated for six different APL antibodies including anticardiolipin (ACL) antibody.

Correlation between peritoneal fluid and serum antiphospholipid antibodies in women with primary infertility.

Objective: To compare the peritoneal and serum levels of antiphospholipid antibodies (aPLs) against the following phospholipids: cardiolipin, L-alpha-phosphatidic acid, L-alpha-phosphatidylethanolamine, 1-alpha-phosphatidyl-DL-glycerol, L-alpha-phosphatidyl-inositol, L-alpha-phosphatidyl-L-serine in immunoglobulin isotypes of G, M, and secretory A.

Patients And Methods: A group of 107 women with primary infertility were immunologically examined. An ELISA method was used for detection of aPLs.

[Profile of antiphospholipid antibodies in various diagnoses associated with reproduction].

The authors investigated antiphospholipid antibodies (APA) against phosphatidic acid, ph-ethanolamine, ph-DL-glycerol, ph-inositol, ph-L-serine and cardiolipine in isotypes IgG and IgM in various diagnoses associated with reproduction. They found that the most varied pattern of high levels of these antibodies is in particular in patients with the diagnosis of intractable infertility (in 26.5% women IgG against cardiolipine, in 24.

Structure and function of IgE myeloma protein VL from an atopic patient.

In a woman suffering from IgE myeloma, hay fever and polyvalent respiratory and skin allergy the IgE monoclonal protein VL was isolated and investigated with respect to structural and functional properties. The amino acid sequence of 22 isolated peptides--especially of the biologically significant C2-C3 part--corresponded with that originally described by Bennich et al. (Immunol Rev 1978;41:3-23; Prog Immunol 1974;13:49-58).

[Zona pellucida antibodies determined by the passive hemagglutination reaction--I. Methods].

Antibodies against the zona pellucida can block the fertilization process. The authors elaborated therefore a feasible examination method for the needs of the Clinic of Immunology of Reproduction of the Gynaecological and Obstetric Department in Plzen. Using three different extraction solubilization methods and chromatographic partition, they obtained a total of 11 porcine antigenic zonal fractions (from 13.

[Immunologic factors in human colostrum and milk].

Background: The mammary gland is at present conceived as an immunological secretory organ, capable of producing specific and non-specific factors transmitted into colostrum or milk.

Methods And Results: Colostrum was collected from the 1st to 3rd day after delivery, milk on the 4th to 19th day after delivery. Colostrum from 42 and milk from 63 mothers was examined.

[Thermoradiotherapy of cancer of the endometrium].

The paper is concerned with the consideration of technical potentialities of local UHF-hyperthermia with regard to combined radiotherapy of endometrial cancer. The authors performed comparative analysis of thermoradiotherapy of a tumor and adjacent normal tissues and of combined radiotherapy as an independent method. The results of this investigation have shown that a decrease in a single focal dose per fraction, taking into account the DMF of hyperthermia in thermoradiotherapy, the same therapeutic effect on a tumor being preserved like in radiotherapy, reduces the frequency of radiation reactions of adjacent normal organs and tissues.

[Plasmapheresis--a therapeutic method for immunity-related infertility?].

In a 30-year-old woman with primary idiopathic sterility the immunological cause of infertility was assessed (spermagglutination antibodies in serum and in the ovulation mucus of the uterine cervix). The patient was treated without success by condom therapy, general and local immunosuppression. Therefore treatment by means of membrane separation plasmapheresis was started during two preovulation periods.

[The effect of general anesthesia on memory in experimental animals].

Full recovery of short-term memory (maximum time of delayed reactions) and of the structure of situational conditioned reflex and differentiation inhibition in dogs is observed in 1.5-2.5 h after tranquil-anaesthesia (diazepam-ketamine in doses of 0.

[The role of different neuromediator systems in regulating the reproduction of memory engrams in rats].

In experiments on 796 white rats the role was studied of various neurotransmitter systems (choline-, adreno-, serotonin-, dopamine-, and GABA-ergic) in regulation of processes of engrams reproduction, determined by tests of conditioned passive- and active-defensive reactions in comparison with changes of spontaneous motor activity and oriented reaction. Correlations are analyzed between the changes of animals behavioural reactions in conditions of pharmacological control of activity of the studied neurotransmitter systems.

[The significance of the antihypoxic properties of etimizol in its nootropic action].

The antihypoxic effects of etimizol and other nootropics are analysed on various experimental models of hypoxia and during bicycle ergometric exercise in healthy volunteers. Etimizol was shown to relieve amnesia effectively in the origin of which there is the hypoxic component (hypobaric hypoxia, actinomycin D, mechanical injury of the brain). The advantage of etimizol as compared to other nootropics with similar action (meclofenoxate, piracetam) is its high effectiveness at a single administration.

[Long-term results of combined radiation therapy of cancer of the cervix].

The authors presented 5-year results of combined radiation therapy of 217 patients with stage I-II cervical cancer using 60Co high energy sources on the Agat-B unit for intracavitary irradiation. Intracavitary therapy with fractions of 10 and 5 Gy was compared. No significant correlation was found between a therapeutic effect and a fraction value.

[GABA-ergic mechanisms of the action of ketamine on the structures of the hippocampo-reticulo-neocortical system of the brain].

In freely mobile rabbits with electrodes preliminarily implanted in the brain structures ketamine (5 mg/kg intravenously or 20 mg/kg intramuscularly) exhibited properties of GABA receptor agonist decreasing the direct excitability of the mesencephalic reticular formation and periaqueductal gray matter and reducing their activating effect on the frontal cortex (FC) and dorsal region of the hippocamp (DH). GABA-mimetics (muscimol, baclofen, aminooxyacetic acid) as well as GABA system antagonists (bicuculline, thiosemicarbazide) attenuated ketamine action at the level of DH and especially FC.

[Neurophysiological mechanisms of the action of GABA-tropic agents on brain structures].

The effect of systemic administration of agonists and antagonists of GABA system on excitability and intracentral relationships of the brain structures was found during chronic experiments on rabbits with bipolar electrodes implanted in the region of the frontal cortex, dorsal hippocampus, midbrain reticular formation and central gray matter. The changes of the functional state of the cerebral formations were suggested to be related to a different degree of the involvement of GABA receptors of types A and B.

[Role of adrenergic mechanisms in regulating memory engram retrieval and energy metabolism in the brain structures of the rat].

In conditions of stimulation of adrenergic mechanisms with ephedrine, there was an improvement of the memory engrams reproduction (MER). Blockade of the alpha-adrenergic system by pyrroxan more expressively impeded reproduction of a passive defensive habit in comparison with the action of a beta-adrenolytic, the obsidan. Stimulation of MER processes was accompanied by a parallel activation of glucose-6-phosphate- and 6-phosphategluconatedehydrogenases of pentose path in the tissues of the frontal area of the cerebral cortex and pons Varolii.

[Immediate and near-term results of radiotherapy of uterine cancer using highly active 252Cf sources].

The paper is concerned with the results of therapy of 35 patients with Stage I-II uterine body cancer with the method of combined modality radiotherapy using neutron radiation of high energy 252Cf sources on the ANET-B unit with a system of automatic remote-control delivery of sources for intracavitary irradiation. The RBE was 3. A 3-12 mos.

[Optimization of intracavity irradiation in the treatment of cancer of the corpus uteri using high-activity sources].

The paper is concerned with an analysis of the results of concomitant therapy of corpus uteri cancer, Stage I-II, in 70 patients with relation to the regimen of intracavitary irradation using high energy sources on the Agat-B afterloading unit. Therapeutic efficacy, the frequency and severity of radiation complications were evaluated depending on the regimen of contact irradiation. Short-term results of concomitant radiation therapy were improved when passing over from a scheme of intracavitary irradiation of 10 Gy a week to schemes of dynamic fractionation and twofold irradiation a week at a dose of 5 Gy.