Peroxide treatment changes activity of non specific esterase in vascular endothelium of isolated pig aorta in vitro.

The effect of hydrogen peroxide on certain enzymes contained in endothelial cells was investigated in vitro. Specifically in this study the influence of hydrogen peroxide on the non-specific esterase activity in endothelial cells of the perfused pig aorta was examined. We perfused an isolated segment of the pig aorta for 10 h and added 5, 10, 12, 15 ml 10% hydrogen peroxide to 100 ml perfused medium.

The effect of peroxides on the vascular endothelium of isolated pig aorta in vitro.

The effect of peroxide on endothelial cells (perfused pig aorta) was examined using an in vitro perfusion model. Hydrogen peroxide was added to the perfusion medium (pig serum together with a buffer solution) which was expected to lead to an increased oxidation of lipids and lipoproteins. Oxidation processes of this type play a decisive role in the pathogenesis and progression of arteriosclerosis.

Description of a simple method to study some aspects of the pathogenesis of arteriosclerosis by perfusion of arterial segments.

A perfusion apparatus is described which permits the perfusion of arterial segments of about 5 cm in length. It works under physiological conditions. During the perfusion, temperature and pressure constancy or pressure variation, respectively, pulsation, sufficient oxygen supply, and high sterility are guaranteed.

Crystallinity of mineral deposited in arterial walls in the course of arteriosclerosis in diabetics and in patients with normal carbohydrate metabolism.

A comparison was made of the amount and crystallinity of mineral deposited in the course of the arteriosclerosis in arterial walls in diabetic patients and in individuals with normal carbohydrate metabolism. The macroscopically unchanged tunica intima, fibro-lipidic plaques and bone-like lamellae were taken from aorta thoracalis , aorta abdominalis, arteriae femorales and arteriae coronariae in the course of the autopsy of 17 insulin dependent diabetic individuals and of 9 persons with arteriosclerosis, but with normal carbohydrate metabolism, called control group. The total inorganic constituents in the three kinds of samples were determined by ashing of dried samples at 600 degrees C for 6 h.

[Histochemical determination of sorbitol dehydrogenase in the rat kidney. Indicator histochemical, immunohistochemical and microelectrophoretic studies].

By comparative indicator- and immunohistochemical technique we have investigated the distribution of sorbitol dehydrogenase (SoDH) in rat kidney. In this connection we have tested some parameters which influenced the histochemical demonstration of SoDH activity by tetrazolium salt indicator technique (e.g.

[Specificity of the histochemical determination of sorbitol dehydrogenase--comparative indicator and immunohistochemical studies on the rat kidney].

We have investigated the distribution of sorbitol dehydrogenase by comparative indicator- and immunohistochemical technique in rat kidney. In this connection we have tested some parameters which influence the histochemical demonstration of SoDH-activity by tetrazolium salt indicator technique (e.g.

[On the localization of sorbitol dehydrogenase in the rat kidney (author's transl)].

We have investigated the localization and demonstration of sorbitol dehydrogenase activity in the rat kidney by comparative histochemical-electrophoretical technique. Under histochemical conditions an adequate demonstration of the activity of the sorbitol dehydrogenase in native sections is possible by membrane incubating technique in presence of PMS and KCN. In the glomerulum the strong and uniform reaction with regard to the specifity is not clear.