Antibody and siRNA Nanocarriers to Suppress Wnt Signaling, Tumor Growth, and Lung Metastasis in Triple-Negative Breast Cancer.

The paucity of targeted therapies for triple-negative breast cancer (TNBC) causes patients with this aggressive disease to suffer a poor clinical prognosis. A promising target for therapeutic intervention is the Wnt signaling pathway, which is activated in TNBC cells when extracellular Wnt ligands bind overexpressed Frizzled7 (FZD7) transmembrane receptors. This stabilizes intracellular β-catenin proteins that in turn promote transcription of oncogenes that drive tumor growth and metastasis.

Combination cancer imaging and phototherapy mediated by membrane-wrapped nanoparticles.

Cancer is a devastating health problem with inadequate treatment options. Many conventional treatments for solid-tumor cancers lack tumor specificity, which results in low efficacy and off-target damage to healthy tissues. Nanoparticle (NP)-mediated photothermal therapy (PTT) is a promising minimally invasive treatment for solid-tumor cancers that has entered clinical trials.

Ascorbate reacts with singlet oxygen to produce hydrogen peroxide.

Singlet oxygen is a highly reactive electrophilic species that reacts rapidly with electron-rich moieties, such as the double bonds of lipids, thiols, and ascorbate (AscH-). The reaction of ascorbate with singlet oxygen is rapid (k = 3 x 10(8) M(-1) s(-1)). Here we have investigated the stoichiometry of this reaction.

Ascorbate enhances the toxicity of the photodynamic action of Verteporfin in HL-60 cells.

As a reducing agent, ascorbate serves as an antioxidant. However, its reducing function can in some settings initiate an oxidation cascade, i.e.

Effect of carnosine on Drosophila melanogaster lifespan.

A positive dose-dependent effect of carnosine (beta-alanyl-L-histidine) on the lifespan of male Drosophila melanogaster flies was shown. The mean lifespan of male flies receiving 200 mg/liter carnosine approached that of females. At the same time carnosine had no effect on the lifespan of female flies.

Peculiarities of carnosine metabolism in a patient with pronounced homocarnosinemia.

The article describes a case of homocarnosinemia with increased liquor and plasma content of homocarnosine, increased urinary excretion of homocarnosine, and low activity of serum carnosinase. These metabolic disturbances were accompanied by moderate neurological disorders. Changes in carnosine metabolism in family members were less pronounced and not accompanied by neuropathological symptoms.

Antioxidant systems in tissues of senescence accelerated mice.

Significant decrease in the level of lipid antioxidants (measured from the kinetics of the induced chemiluminescence in brain homogenate) and of the hydrophilic antioxidant carnosine as well was observed in the brain of 14-16-month-old mice of SAMP1 line, which is characterized by accelerated accumulation of senile features, in comparison with the control line SAMR1. In the brain of SAMP1 animals the activity of cytosolic Cu/Zn-containing superoxide dismutase (SOD) was reduced, while the activity of membrane-bound Mn-SOD was at an extremely low level. The activity of glutathione-dependent enzymes (glutathione peroxidase, glutathione reductase, and glutathione transferase) did not differ in the brain of SAMP1 and SAMR1 animals, and catalase activity was similarly low in both cases.

Effect of nitroso compounds on Na/K-ATPase.

Thiol containing NO.-derivatives were found to inhibit the activity of brain and kidney Na/K-ATPase. S-Nitrosogluthatione demonstrated only minor inhibiting activity, while dinitrosyl iron complexes (DNIC) with cysteine or glutathione were much more effective.

[Effect of extremely low frequency electromagnetic radiation and ultra-violet radiation on aggregation of thymocytes and erythrocytes].

Electromagnetic radiation of superhigh frequencies (46.12 and 46.19 GHz, 0.

[Isolation of carnosine synthetase from animal and human muscles].

Carsinosine synthetase was comparatively estimated in the homogenates of chicken sternal muscle and human skeletal and heart muscles. Partially purified enzyme was obtained from chicken muscle, showing its activity of 60 nmoles carnosine/mg per min. The levels of carnosine synthesized was measured by three different methods: column chromatography on Dowen 50 x 8 resin, and thin-layer chromatography on Fixion or Silufol plates.

[Change in the aggregation ability of rabbit thymocytes under the combined effect of UV-radiation and extremely high frequency radiation].

The number of thymocytes, which have ability to aggregate with homologous erythrocytes, increases under action of the 254 nm UV-radiation at low fluences of 100-350 J/m2 similar action of ultra high frequency (UHF) radiation (46.12 GHz). The number of the active cells decreases during their incubation in the dark after irradiation, i.

[Place of the pathology service in the municipal-insurance public health system in the Kemerovo district].

Role and place of pathology service within the system of the insurance medicine is considered. The problems of financing and relations between bureaus of pathology and health departments are discussed.

[Characteristics of foot blood supply in rigidity of the great toe].

By means of angiography, made in 20 patients with rigidity of the first toes due to osteoarthrosis, there have been revealed chronic deficiency of blood circulation in the foot tissues, especially in its antero-internal section. Blood circulation deficiency is due either to the congenital anomaly of the foot vessels or to the consequences of their traumatic injuries. Blood supply deficiency results in degenerative-dystrophic changes of the first metatarsophalangeal articulations with development of their rigidity, aggravated by post-ischemic contracture of the foot muscles.

[Case of trichinosis with lethal outcome due to consumption of brown bear meat in the Krasnoyarsk Territory].

One autopsy case of trichinellosis (in a man of 22) with extensive involvement of the locomotor apparatus (secondary infectious-allergic polymyositis) is presented. Group infection with trichinellosis occurred by eating of insufficiently treated meat of a brown bear which confirms the existence of a natural focus of trichinellosis in the Krasnoyarsky kray.

[Acute untreated idiopathic polymyositis with cardiac lesions].

An autopsy observation of acute idiopathic polymyositis with the involvement of the sceletal musculature and the heart in a man of 23 who had not been treated with corticosteroids is presented. The main morphological lesions were found in the cross-striated muscles and myocardium. They were characterized by deep degeneration, necrosis, and atrophy of muscle fibers, multiplication of sarcolemma nuclei, massive lymphoplasmo-histiocytis infiltration of the interstitial tissue, active resorbtion by macrophages of the dead muscle cells, the occurrence of giant cells.