Human Diploid Fibroblast Cell Lines - a Model System for Studying Immunodulatory Properties of Modern Immunobiological Drugs and Viruses.

The immunomodulatory properties of immunobiological drugs Glutoxim and Phosprenyl we well as vesicular stomatitis virus and inactivated tick-borne encephalitis vaccine virus were studied using human diploid fibroblast cell line from the collection of M. P. Chumakov Federal Research Center for Research and Development of Immunobiological Products.

Selection of Domestic Cell Lines for the Creation of Diagnostic and Preventive Preparations against Enterovirus 71.

We studied the sensitivity of domestic proprietary human and animal cell lines from the collection of M. P. Chumakov Federal Scientific Center for Research and Development of Immuneand-Biological Products to infection with different enterovirus 71 strains.

[Antiviral activity of phosprenyl and gamapren against infection caused by influenza a (H5N1) virus in cell culture].

The antiviral activity of Phosprenyl and Gamapren in vitro against highly pathogenic strain of avian influenza H5N1 virus was studied. Inoculation of the virus to the susceptible cell culture led to development of the cytopathogenic effect. Preliminary introduction of Phosprenyl and Gamapren an hour prior to infecting the cells with virus 10.

[Comprehensive assessment of the health of students with different state of the musculoskeletal system].

Priority health indices (the morbidity rate, the resistance status, the physical development, the orthopedic status, the level of bone mineralization, functional reserves of the body, health groups) in schoolchildren aged 7-18 years with a different state of the musculoskeletal system (MSS), learning in secondary schools were studied with the aim of the development of prevention programs. The high incidence of MSS diseases was determined to be combined with a high level of overall morbidity rate, on separate classes--with diseases of the nervous, endocrine, digestive systems, increasing with age ofthe student. Comprehensive assessment of health is much worse in schoolchildren with functional MSS diseases, as well as in girls.

[Antiviral activity and possible mechanisms of action of moraprenil phosphates during experimental infection caused by herpes simplex type 1 virus].

Aim: Study of antiviral activity of moraprenil phosphates (MPP) against herpes simplex type 1 virus (HSV1) in vitro and during experimental infection caused by HSV1 in mice.

Materials And Methods: Activity of MPP in vitro was tested by the ability to suppress formation of symplasts in VERO cells infected with HSV1, strain VR-3. A series of MPP that suppress virus-induced symplast-formation by 30 times was selected for in vivo experiments.

Efficacy and safety of pidotimod in the prevention of recurrent respiratory infections in children: a multicentre study.

Acute respiratory infections (ARI) still represent a big challenge for paediatricians, especially in those children defined as "ailed" as they are more susceptible to such kinds of disease. In this paediatric population, the immune system is still under-developed with an evident alteration in cytokine levels. A clinical study was carried out in 5 sites in Russia with the intention to enroll children particularly susceptible to contract respiratory infections (defined as "ailing"), assigning them to a treatment group with pidotimod in comparison with a control group, treating them for 30 days and observing the reduction in the number of ARI episodes throughout the follow-up period (6 months).

[Adjuvants in modern medicine and veterinary].

The review is dedicated to immunologic adjuvants--various natural and synthetics substances that are added to vaccines for stimulation of specific immune response, but they do not induce specific response themselves. Critically important is the selection of the correct adjuvants, for which mechanisms of effect on immune system are studied the most. The majority of these mechanisms as well as physical-chemical and biological features of modern adjuvants are analyzed in the review.

[Multidirectional effect of MIF and sodium polyprenyl phosphate on the course of experimental flavivirus infection in mice].

Aim: Study of macrophage migration inhibiting factor (MIF) effect after intracerebral administration on the course of experimental infection induced in mice by tick borne encephalitis virus (TEV), and study of sodium polyprenyl phosphate (PPP) and/or antibodies against MIF on the course of this infection against the background of MIF administration.

Materials And Methods: Phosprenil preparation was used as a source of PPP. PPP was administered intracerebrally.

[Experimental study of the phenomenon of antibody dependent tick-borne encephalitis virus infectivity enhancement in vitro].

Phenomenon of antibody dependent tick-borne encephalitis virus (TBEV) infectivity enhancement in the human monocytic cell culture J-96 has been studied. Three specific commercial sera that are generally used for therapy and prevention of TBE in humans were used. Results showed that enhancement of TBEV infectivity was markedly apparent following use of 2 out of 3 studied sera.

[Antiviral and immunomodulating activity of polyprenyl phosphates in viral infections].

Expressed antiviral activity of Fortepren (FP) and Gamapren (GP), polyprenyl phosphate (PPP)-contaning agents, was demonstrated in experiments on mice infected with the human herpes simplex virus, type 1 (HSV1) or the vernal encephalitis virus (VEV). Since both the viral infections are of great social significance, the PPP-containing agents should be considered prospective for the medical practice. The experimental data suggested that both the drugs considerably inhibited the VEV infectiousness in the susceptible cell culture.

[Moraprenylphosphates suppress reproduction of Taylor murine encephalomyelitis virus and accumulation of VP3 viral protein in susceptible cell cultures BHK-21 and P388D1].

Influence of moraprenylphosphates (phosphorylated polyprenol of plant origin) upon the accumulation of Taylor murine encephalomyelitis virus VP3 protein in the susceptible cell cultures was studied. It has been shown that moraprenylphosphates inhibited the accumulation of VP3 at early stages of infectious process. Moraprenylphosphates were found to decrease infectivity of the virus as well as virus-induced cellular apoptosis.

[The correction action of Phosprenyl and Gamavit on the functional activity of mouse peritoneal macrophages in response to high doses of Staphylococcus aureus alpha-toxin].

The study of the functional activity of peritoneal macrophages of BALB/c mice at different stages of the toxic action caused by S. aureus alpha-toxin (ST) was carried out. The analysis of the dynamics of toxic reaction revealed the main critical points of triggering necrotic processes: the first hour and day 2.

[Caries prevention in children during orthodontic treatment].

The study allowed establishing imbalance of immune system during orthodontic appliance treatment. It follows in 1, 5 month after fixation of appliance and in the final period of treatment (15 months after fixation of breakers). These can be considered "critical" and it is necessary to make immune-correctional therapy and to prescribe measures of caries prevention.

[Local features of temporal organization of tissue mast cells in the dura mater of mature rat brain].

Rhythms of functional activity of mast cells were studied in frontal, parietal and occipital areas of dura mater of mature rats. Chronological organization of mast cells dura mater was established. Circadian component dominated in all rhythms studied.

[Microorganism persistence in hematopoietic tissue: means for detecting it and its significance].

Many microorganisms are capable of prolonged persistence in the marrow. In this study, carried out by the method of negative selection based on the treatment of mouse marrow cells with specific antimicrobial sera and complement, Mycoplasma arthritidis and L-forms of group B streptococci were found to be capable of persisting in the marrow in close association with the late category of clonogenic precursor cells, CFU-7, as well as, to a lesser extent, with late erythroid precursors, CFUe. Early colony-forming cells, CFUs-12 and PFUe, as well as granulocyto-macrophagal precursors, CFUgm, did not practically express antigens to the given infective agents on their surface.

[Localization of biogenic monoamines in the dura mater of the rat brain].

Quantitative and qualitative composition of catecholamines (noradrenaline and dophamine) and indolalkylamines (serotonin and tryptamine) and their localization have been studied in cells and neural fibers of the rat dura mater. Luminiscent-cytophotometric and electron microscopic methods have revealed two types of cells depositing biogenic monoamines. As demonstrate the experiments with rausedil injection, monoaminocytes of the first type (mast cells) contain predominantly indolalkylamines and a small amount of dophamine.

[Circadian rhythm in the functional activity of mast cells of the dura mater of rat brain].

The Falk-Hillarp method was used to study circadian activity of the rat dura mater mast cells and the content of monoamines in them. The quantitative changes in the mast cells are described as time-dependent. The data obtained were statistically analyzed to disclose the latent periodicity of the processes occurring in the mast cells.

[Age and individual characteristics in the structure of the celiac trunk in man].

The individual and developmental features of the structure of the celiac trunk were studied in 155 human corpses of either sex, age and habitus. The method used was the dissection preceded by the injection of vessels with roentgen-contrast masses and roentgenography. The individual and developmental features of the level of branching the celiac trunk off from the abdominal part of the aorta have been revealed, as well as the angle of its branching, the length and diameter of the celiac trunk and the form of its ramification.