Effects of mechanical stress on human oral mucosa-derived cells.

Objectives: Placement of a denture results in the application of mechanical stress (MS), such as occlusal force, onto the oral mucosa beneath the denture. To better understand the molecular mechanism underlying MS-induced inflammation in the oral mucosa, we examined the impact of MS on human oral epithelial cells (HO-1-N-1) and human fibroblasts (HGFs) in this study.

Materials And Methods: MS was applied on HO-1-N-1 and HGFs using a hydrostatic pressure apparatus.

Transplantation of dental pulp-derived cell sheets cultured on human amniotic membrane induced to differentiate into bone.

Objective: The usefulness of the amniotic membrane as a cell culture substrate has led to its use in the development of dental pulp-derived cell sheets. We induced osteoblastic differentiation of dental pulp-derived cell sheets and conducted histological and immunological examinations in addition to imaging assessments for regeneration of bone defects.

Methods: Dental pulp cells were obtained by primary culture of the dental pulp tissue harvested from extracted wisdom teeth.

Effects of far infrared radiation by isotropic high-density carbon on the human oral mucosa.

Objectives: Wound healing of the oral mucosal epithelium through the application of far infrared radiation emitted by isotropic high-density carbon was investigated in order to clarify the preventive and therapeutic effects of isotropic high-density carbon on oral mucosal injury.

Materials And Methods: A carbon massager with an isotropic high-density carbon tip was used. Far infrared radiation was applied to the human buccal mucosal squamous cell carcinoma cell line, HO-1-N-1 using a carbon massager, and cell growth factors and heat shock protein levels were measured using real-time RT-PCR and ELISA.

Accumulation of microcystins in various organs of the freshwater snail Sinotaia histrica and three fishes in a temperate lake, the eutrophic Lake Suwa, Japan.

So far, there has been only one study to examine microcystin (MC) contents in various organs of snails in a subtropical Chinese lake. In this study, tissue distribution and seasonal dynamics of MC-RR and -LR were investigated in various organs of a freshwater snail (Sinotaia histrica) in a temperate eutrophic lake, Lake Suwa, Japan. Accumulation of microcystins in some fish was also investigated.