Improved HLA-based prediction of coeliac disease identifies two novel genetic interactions.

Human Leucocyte Antigen (HLA) testing is useful in the clinical work-up of coeliac disease (CD) with high negative but low positive predictive value. We construct a genomic risk score (GRS) using HLA risk genotypes to improve CD prediction and guide exclusion criteria. Imputed HLA genotypes for five European CD case-control GWAS (n > 15,000) were used to construct and validate an interpretable HLA-based risk model (HDQ), which shows statistically significant improvements in predictive performance upon all previous HLA-based risk models.

Dissipative Distillation of Supercritical Quantum Gases.

We experimentally realize a method to produce nonequilibrium Bose-Einstein condensates with condensed fraction exceeding those of equilibrium samples with the same parameters. To do this, we immerse an ultracold Bose gas of ^{87}Rb in a cloud of ^{39}K with substantially higher temperatures, providing a controlled source of dissipation. By combining the action of the dissipative environment with evaporative cooling, we are able to progressively distil the nonequilibrium Bose-Einstein condensate from the thermal cloud.

Dosimetric evaluation of vaginal cuff brachytherapy planning in cervical and endometrial cancer patients.

Purpose: The aim of the study was to perform a prospective analysis of dosimetric consequences of rectal enema administration before vaginal cuff brachytherapy (VCB), the dose distribution in organs at risk (OARs), and the presence of air gaps (AGs) in patients with cervical or endometrial cancer.

Material And Methods: In total, 75 patients treated in 2019 were randomly divided into two groups including 38 patients with and 37 without an enema before VCB. All patients received post-operative high-dose-rate (HDR).

Morphology of sesamoid bones in keyboard musicians.

Background: The sesamoid bones are small, usually oval bone structures often found in joints and under the tendons. Although their precise function is not fully understood, it is agreed upon that they protect the joints and make movements faster and less energy consuming. Sesamoid bones are found in hands, especially around first, second and fifth metacarpophalangeal joint and the interphalangeal joint of the thumb.

Near Infrared Phosphorescent Dinuclear Ir(III) Complex Exhibiting Unusually Slow Intersystem Crossing and Dual Emissive Behavior.

A dinuclear iridium(III) complex shows dual emission consisting of near infrared (NIR) phosphorescence (λ = 714 nm, CHCl, = 300 K) and green fluorescence (λ = 537 nm). The NIR emission stems from a triplet state (T) localized on the ditopic bridging ligand (LC). Because of the dinuclear molecular structure, the phosphorescence efficiency (Φ = 3.

Tris(ferrocenylmethidene)sumanene: synthesis, photophysical properties and applications for efficient caesium cation recognition in water.

The synthesis of a sumanene derivative bearing three ferrocenyl substituents is presented. This conjugated compound is solution-processable, shows red-light emission with high fluorescence quantum yield and can be used for the construction of the first buckybowl-based sensor for the selective and effective recognition of caesium cations (Cs) in aqueous solution.

Characteristics of capercaillie (Tetrao urogallus) semen analysed with flow cytometry combined with fertility results.

In order to increase the reproductive indices of capercaillie kept in closed breeding facilities, it is necessary to constantly expand the methods of better understanding the characteristics of sperm and their fertilizing potency. The aim of the study was to analyse selected features of capercaillie sperm using flow cytometry and their connection with fertility results. The study included five males, three of which were kept in a family group with eight females and two were kept alone.

Ethanol water: influence of the terminal functional group of the alkyl chain and environment of the self-assembly process on electron transport through the thiol layer.

Self-assembly of alkanethiol chains on metallic surfaces is a spontaneous process which leads to the formation of highly ordered layers. However, the organization of the thiol chains on the surface strongly depends on the intermolecular interactions between the terminal groups in the chain. The solution environment also plays an important role.

MCM-22, MCM-36, and ITQ-2 Zeolites with Different Si/Al Molar Ratios as Effective Catalysts of Methanol and Ethanol Dehydration.

MCM-22, MCM-36, and ITQ-2 zeolites with the intended Si/Al molar ratios of 15, 25, and 50 were synthetized and tested as catalysts for dehydration of methanol to dimethyl ether and dehydration of ethanol to diethyl ether and ethylene. The surface concentration of acid sites was regulated by the synthesis of zeolite precursors with different aluminum content in the zeolite framework, while the influence of porous structure on the overall efficiency of alcohol conversion was analyzed by application of zeolitic materials with different types of porosity-microporous MCM-22 as well as microporous-mesoporous MCM-36 and ITQ-2. The zeolitic samples were characterized with respect to their: chemical composition (ICP-OES), structure (XRD, FT-IR), texture (N sorption), and surface acidity (NH-TPD).

Evaluation of bioactive compounds and antioxidant capacity of edible feijoa ( (O. Berg) Burret) flower extracts.

Abstract: The research aimed to evaluate the polyphenolic composition and the antioxidant capacity of edible extracts of feijoa ( (O. Berg) Burret) flowers. Phenolic compounds of whole feijoa flower (FM), feijoa petals (PM) and feijoa petals juice (PJ) were identified by high-performance liquid chromatography coupled with electrospray mass spectrometry and quantified by liquid chromatography coupled with ultraviolet/visible detection.

Mutation-Independent Allele-Specific Editing by CRISPR-Cas9, a Novel Approach to Treat Autosomal Dominant Disease.

CRISPR-Cas9 provides a tool to treat autosomal dominant disease by non-homologous end joining (NHEJ) gene disruption of the mutant allele. In order to discriminate between wild-type and mutant alleles, Streptococcus pyogenes Cas9 (SpCas9) must be able to detect a single nucleotide change. Allele-specific editing can be achieved by using either a guide-specific approach, in which the missense mutation is found within the guide sequence, or a protospacer-adjacent motif (PAM)-specific approach, in which the missense mutation generates a novel PAM.

Protein exchange is reduced in calcium-independent epithelial junctions.

Desmosomes are cell-cell junctions that provide mechanical integrity to epithelial and cardiac tissues. Desmosomes have two distinct adhesive states, calcium-dependent and hyperadhesive, which balance tissue plasticity and strength. A highly ordered array of cadherins in the adhesive interface is hypothesized to drive hyperadhesion, but how desmosome structure confers adhesive state is still elusive.

The desmosome as a model for lipid raft driven membrane domain organization.

Desmosomes are cadherin-based adhesion structures that mechanically couple the intermediate filament cytoskeleton of adjacent cells to confer mechanical stress resistance to tissues. We have recently described desmosomes as mesoscale lipid raft membrane domains that depend on raft dynamics for assembly, function, and disassembly. Lipid raft microdomains are regions of the plasma membrane enriched in sphingolipids and cholesterol.

Effect of semen extenders on viability of ISA Brown and Hubbard Flex roosters' sperm stored for 24 h.

Artificial insemination is used in almost 95% of turkey reproductive flocks and is becoming more important in chickens, particularly broiler breeders, as well as in assisted reproduction of wild birds kept in breeding centers. Diluted semen is recommended for artificial insemination. Pooled semen samples collected twice a week by dorso-abdominal massage from 2 chicken lines: laying-ISA Brown (ISA-B) and meat type-Hubbard Flex (H-F) were divided into 5 parts: neat semen and diluted in 1:2 ratio with 4 extenders: basic EK; EK + 1 μg/mL organic selenium and 8 μg/mL vitamin E; EK + 10 mg/mL of royal jelly; and EK + 0.

Impact of Prebiotics and Synbiotics Administered on the Immune Response against Experimental Antigens in Chicken Broilers.

The effect of the application of selected prebiotics and synbiotics on the humoral immune response against T-dependent (SRBC) and T-independent (dextran) antigens and delayed-type hypersensitivity (DTH) to phytohemagglutinin was studied. On the 12th day of incubation, 800 eggs (Ross 308) were divided into five groups and injected into the egg air chamber with prebiotic inulin (Pre1), Bitos (Pre2), a synbiotic composed of inulin and subsp. IBB SL1 (Syn1), a synbiotic composed of Bitos and subsp.

Female-Male and Female-Female Social Interactions of Captive Kept Capercaillie () and Its Consequences in Planning Breeding Programs.

Capercaillie behavior, both in the wild and in captivity, is poorly known due to this species' secretive way of life. Female-male and female-female social organization and interactions are especially poorly documented. The research was conducted in Capercaillie Breeding Center in Wisła Forestry District where a breeding flock is kept throughout the year.

VE-cadherin endocytosis controls vascular integrity and patterning during development.

Tissue morphogenesis requires dynamic intercellular contacts that are subsequently stabilized as tissues mature. The mechanisms governing these competing adhesive properties are not fully understood. Using gain- and loss-of-function approaches, we tested the role of p120-catenin (p120) and VE-cadherin (VE-cad) endocytosis in vascular development using mouse mutants that exhibit increased (VE-cadGGG/GGG) or decreased (VE-cadDEE/DEE) internalization.

Ferrocene-pyrene conjugates for detection of various monovalent anions in solution.

The ability of ferrocene-pyrene conjugates towards anion recognition still arouses great deal of interest. However, the reported ferrocene-pyrene compounds allow detection of just one anion. Here, for the first time we present new pyrenyl derivatives of ferrocene, which ensure simultaneous detection of various monovalent anions.

New Unsymmetrical Bisacridine Derivatives Noncovalently Attached to Quaternary Quantum Dots Improve Cancer Therapy by Enhancing Cytotoxicity toward Cancer Cells and Protecting Normal Cells.

The use of nanoparticles for the controlled drug delivery to cells has emerged as a good alternative to traditional systemic delivery. Quantum dots (QDs) offer potentially invaluable societal benefits such as drug targeting and biomedical imaging. In contrast, QDs may also pose risks to human health and the environment under certain conditions.

Metallocenyl 7-ACA Conjugates: Antibacterial Activity Studies and Atomic-Resolution X-ray Crystal Structure with CTX-M β-Lactamase.

The conjugation of organometallic groups to current β-lactam antibiotics is a field of increasing study due to the ability of certain organometallic groups to enhance the antibiotic potency of these drugs. Herein, we report the antibacterial properties of two metallocenyl (ferrocenyl and ruthenocenyl) 7-aminocephalosporanic acid (7-ACA) antibiotic conjugates. Continuing a trend we found in our previous studies, the ruthenocenyl conjugate showed greater antibacterial activity than its ferrocenyl counterpart.

Pan-mammalian analysis of molecular constraints underlying extended lifespan.

Although lifespan in mammals varies over 100-fold, the precise evolutionary mechanisms underlying variation in longevity remain unknown. Species-specific genetic changes have been observed in long-lived species including the naked mole-rat, bats, and the bowhead whale, but these adaptations do not generalize to other mammals. We present a novel method to identify associations between rates of protein evolution and continuous phenotypes across the entire mammalian phylogeny.

Arbuscular Mycorrhiza Changes the Impact of Potato Virus Y on Growth and Stress Tolerance of L. .

Under the field conditions crop plants interact with diverse microorganisms. These include beneficial (symbiotic) and phytopathogenic microorganisms, which jointly affect growth and productivity of the plants. In last decades, production of potato ( L.

Catalytic and photocatalytic oxidation of diphenyl sulphide to diphenyl sulfoxide over titanium dioxide doped with vanadium, zinc, and tin.

Samples of TiO (P25) doped with zinc, tin, and vanadium, thermally treated at 550 °C for 6 h, were tested as catalysts and photocatalysts for the oxidation of diphenyl sulphide to diphenyl sulfoxide and sulfone, using hydrogen peroxide as an oxidation agent. Thermal treatment of pure TiO and its vanadium-doped forms resulted in a decrease of anatase and an increase of rutile content. The opposite effect was observed for TiO doped with zinc or tin, where thermal treatment resulted in the rutile to anatase phase transition.

A dependency between PRL gene polymorphism and breeding parameters in Polish Black-and-White Holstein-Friesian cows.

The aim of this work was to determine the dependency between polymorphism in the locus g.8398G > A and fertility rates, bodyweight of cows and calves, the course of parturition and viability of calves in Polish Black-and-White Holstein-Friesian cattle. Three genotypes (AA, AG, and GG) were identified with a frequency of 0.