Human TRIM14 protects transgenic mice from influenza A viral infection without activation of other innate immunity pathways.

TRIM14 is an important component of innate immunity that defends organism from various viruses. It was shown that TRIM14 restricted influenza A virus (IAV) infection in cell cultures in an interferon-independent manner. However, it remained unclear whether TRIM14 affects IAV reproduction and immune system responses upon IAV infection in vivo.

[Efficacy and safety of new oral anticoagulants as part of triple antithrombotic therapy in patients with atrial fibrillation and acute coronary syndrome. Data from an observational study].

Aim To study efficacy and safety of a triple antithrombotic therapy with direct oral anticoagulants (DOAC) versus warfarin in patients with atrial fibrillation after acute coronary syndrome, for 12 months following discharge from the hospital.Materials and methods This single-site cohort, prospective, observational study performed at the Regional Vascular Center 2 of the N.A.

In Vivo Comparison of Chlorine-Based Antiseptics versus Alcohol Antiseptic for Surgical Hand Antisepsis.

Despite being commonly used as effective preparation for surgical hand antisepsis, alcohol solutions have major drawbacks, such as drying effect, emergence of hand eczema, and other diseases. This study aimed to demonstrate the effectiveness of sodium hypochlorite (NaOCl) and hydrogen peroxide (HO) as antiseptic in comparison to single sodium hypochlorite and 70% ethanol. In 5-day tests, the effects of 3 antiseptics were established according to standard test methods.

[Multifactorial Prognostication of the Development of Stent Thrombosis in Patients with Acute Coronary Syndrome after Percutaneous Coronary Intervention on the background of Dual Antiplatelet Therapy].

Aim: to identify predictors of stent thrombosis in patients with acute coronary syndrome (ACS) after percutaneous coronary intervention (PCI) for 12 months by creating a miathematical logistic regression model to optimize rehabilitation, secondary prevention of ischemic events in the first year after ACS, as well as a personalized approach to treatment.

Materials And Methods: The analysis used data from the hospital register, which contained information on all PCIs, performed in the Semashko hospital between September 2016 and August 2018 (2378 patients). For this study we selected a sample of 183 ACS patients (146 men and 37 women) after PCI: 25 with definite stent thrombosis confirmed by repeated coronary angiography (CAG) (the main study group), and 158 without developing definite stent thrombosis (the comparison group) according to the observation for 12 months.

[Double antiplatelet therapy in patients with acute coronary syndrome after percutaneous coronary intervention: individual efficacy and hemorrhagic safety of P2Y12 blockers of ticagrelor and clopidogrel in actual clinical practice].

Aim: To study the comparative efficacy and safety of clopidogrel and ticagrelor in the "double" antiplatelet therapy (DATT) in patients with acute coronary syndrome (ACS) after percutaneous coronary intervention (PCI) in the early and late periods in real clinical practice, and to assess adherence to treatment.

Materials And Methods: The study included 109 patients with ACS, who underwent PCI. Patients were divided into two groups: the 1st group (n=57) - received ticagrelor and the 2nd group (n = 52) - received clopidogrel in the DATT for 12 months.

[Issues of antithrombotic therapy in ACS patients with atrial fibrillation].

The review analyzed current ideas on prevalence and clinical significance of atrial fibrillation following acute coronary syndrome; described modern approaches to administration of antithrombotic therapy; and addressed available clinical studies on the treatment with warfarin and new oral anticoagulants as a part of combination antithrombotic therapy in patients with atrial fibrillation and acute coronary syndrome.

[Major predictors of stent thrombosis in patients with acute coronary syndrome following transcutaneous coronary intervention who received different double antiplatelet therapy].

The review analyzes the reports addressing from different standpoints the issue of stent thrombosis and its major predictors in patients with acute coronary syndrome following transcutaneous coronary interventions. The review focuses on comparative efficacy and safety of ticagrelor and clopidogrel and the effect of double antiplatelet therapy on stent thrombosis. The issue of patients' compliance with double antiplatelet therapy is also discussed.

[Therapy of Arterial Hypertension in Patients With Chronic Heart Failure and Signs of Chronic Kidney Disease With Fixed Perindopril/Amlodipine Combination].

Purpose: to assess efficacy of a fixed combination of perindopril arginine and amlodipine besylate in the treatment of hypertensive patients with chronic heart failure (CHF) and signs of chronic kidney disease (CKD).

Material And Methods: Persons with CKD (n = 53, age 64.5 ± 8.

[Therapy of Arterial Hypertension in Patients With Chronic Heart Failure and Signs of Chronic Kidney Disease With Fixed Perindopril/Amlodipine Combination].

Purpose: to assess efficacy of a fixed combination of perindopril arginine and amlodipine besylate in the treatment of hypertensive patients with chronic heart failure (CHF) and signs of chronic kidney disease (CKD).

Material And Methods: Persons with CKD (n=53, age 64.5+/-8.

Enhanced expression of trim14 gene suppressed Sindbis virus reproduction and modulated the transcription of a large number of genes of innate immunity.

In the present research, we have studied an influence of enhanced expression TRIM14 on alphavirus Sindbis (SINV, Togaviridae family) infection. In the HEK293 cells transfected with human trim14 gene (HEK-trim14), SINV yield after infection was decreased 1000-10,000 times (3-4 lg of TCD50/ml) at 24 h p.i.

RegPrecise 3.0--a resource for genome-scale exploration of transcriptional regulation in bacteria.

Background: Genome-scale prediction of gene regulation and reconstruction of transcriptional regulatory networks in prokaryotes is one of the critical tasks of modern genomics. Bacteria from different taxonomic groups, whose lifestyles and natural environments are substantially different, possess highly diverged transcriptional regulatory networks. The comparative genomics approaches are useful for in silico reconstruction of bacterial regulons and networks operated by both transcription factors (TFs) and RNA regulatory elements (riboswitches).

Trim14 overexpression causes the same transcriptional changes in mouse embryonic stem cells and human HEK293 cells.

The trim14 (pub, KIAA0129) gene encodes the TRIM14 protein which is a member of the tripartite motif (TRIM) family. Previously, we revealed high expression levels of trim14 in HIV- or SIV-associated lymphomas and demonstrated the influence of trim14 on mesodermal differentiation of mouse embryonic stem cells (mESC). In the present work, to elucidate the role of trim14 in normal and pathological processes in the cell, we used two different types of cells transfected with trim14: mESC and human HEK293.

Comparative genomic reconstruction of transcriptional networks controlling central metabolism in the Shewanella genus.

Background: Genome-scale prediction of gene regulation and reconstruction of transcriptional regulatory networks in bacteria is one of the critical tasks of modern genomics. The Shewanella genus is comprised of metabolically versatile gamma-proteobacteria, whose lifestyles and natural environments are substantially different from Escherichia coli and other model bacterial species. The comparative genomics approaches and computational identification of regulatory sites are useful for the in silico reconstruction of transcriptional regulatory networks in bacteria.

Complete genome and proteome of Acholeplasma laidlawii.

We present the complete genome sequence and proteogenomic map for Acholeplasma laidlawii PG-8A (class Mollicutes, order Acholeplasmatales, family Acholeplasmataceae). The genome of A. laidlawii is represented by a single 1,496,992-bp circular chromosome with an average G+C content of 31 mol%.

[Primary generalized amyloidosis with restrictive cardiomyopathy and severe chronic cardiac insufficiency].

The paper reports a case of restrictive cardiomyopathy due to cardiac amyloidosis. Diagnosis of this condition encounters difficulty created by the absence of pathognomonic symptoms of the disease. Major manifestations of amyloid cardiomyopathy are refractive chronic cardiac insufficiency, absence of cardiomyalgia, marked deterioration of diastolic filling of both ventricles, systemic hypotension, and disturbed heart rhythms.

Transcriptional regulation of the methionine and cysteine transport and metabolism in streptococci.

In streptococci, unlike other Firmicutes, methionine biosynthesis is controlled by protein transcription factors, rather than regulatory RNAs. It was observed that most available streptococcal genomes contain orthologs of two transcriptional regulators of the LysR family: MtaR/MetR and CmbR/FhuR. Comparative genomics techniques were applied to identify two binding motifs occurring upstream of genes involved in metabolism and transport of methionine and cysteine and satisfying the LysR family requirements.

[Regulation of methionine/cysteine biosynthesis in Corynebacterium glutamicum and related genomes].

Methionine is an essential amino acid and the universal N-terminal amino acid of proteins. The biosynthesis of methionine is extensively studied in various organisms that could be used in biotechnological production of methionine. Transcriptional regulation of the methionine synthesis in the Corynebacterium glutamicum genome is well studied.

Comparative genomics of transcriptional regulation in yeasts and its application to identification of a candidate alpha-isopropylmalate transporter.

Conservation rates in non-protein-coding regions of five yeast genomes of the genus Saccharomyces were analyzed using multiple whole-genome alignments. This analysis confirmed previously shown decrease in conservation rates observed immediately upstream of the translation start point and downstream of the stop-codon. Further, there was a sharp conservation peak in the upstream regions likely related to the core promoter (-35 bp to +35 bp around TSS) and a conservation peak downstream of the stop-codon whose function is not yet clear.

[Stimulation of regenerative processes and correction of the functional activity of the liver in its partial resection and toxic lesions].

The effects of hepatotropic growth factors (HGFs) and phospholipid drugs on the recovery of functions and the regeneration of the rat liver were studied in CC14-induced toxic damage and after partial hepatectomy (PHE). HGFs isolated from the cytoplasmic cells of the regenerating liver, as well as from the liver of the animals given prodigiozan and from the media taken after culturing the explants of the regenerating liver were found to stimulate DNA synthesis and hepatocytic proliferation following PHE and in the cirrhotic liver. Prodigiozan was shown to induce the formation of HGFs not only in the rat liver following PHE, but in the liver of intact animals.

[The characteristics of the clinical picture in patients with idiopathic mitral valve prolapse and with anomalous chordae tendineae].

95 patients with idiopathic mitral prolapse (IMP) and 20 patients with anomalous chorda (AC) were evaluated clinically. 13 symptoms and syndromes significantly different from control were distinguished in IMP patients. 12 of them indicated the presence of vegetative dystonia.

[The effect of phosphatidylcholine on repair processes in liver cells in acute CCl4 damage].

Effect of phosphatidylcholine, isolated from soy beans and sunflower seeds for laboratory use, on synthesis of RNA, DNA, albumin and content of newly synthesized mRNA in polyribosomes was studied in hepatocytes after chemical hepatectomy produced by single administration of CCl4 into rats; histological and histochemical studies of liver tissue were also carried out. Phosphatidylcholine from these plants was found to prevent hepatocyte dystrophy and necrosis development, to activate the macrophage response and to stimulate reparation inducing synthesis and secretion of the tumor necrosis factor. The rate of highly labelled RNA synthesis, mainly mRNA, decreased in liver tissue of rats treated with CCl4, was increased approximately 2-fold after the phospholipid administration.

P1,P3-bis(5'-adenosyl)triphosphate (Ap3A) as a substrate and a product of mammalian tryptophanyl-tRNA synthetase.

Bovine tryptophanyl-tRNA synthetase (TrpRS, E.C.6.

Nucleoside triphosphatase activity associated with the N-terminal domain of mammalian tryptophanyl-tRNA synthetase.

Bovine tryptophanyl-tRNA synthetase (EC 6.1.1.