Publications by authors named "Kousuke Inamura"
Article Synopsis
- Human pluripotent stem cell-derived liver organoids (HLOs) show promise for liver regenerative therapy, requiring large amounts of hiPSC-derived liver cells for transplantation.
- A new dialysis-based medium conditioning method was developed to enhance the hepatic differentiation of hiPSCs by efficiently accumulating essential growth factors.
- This approach allowed for high-density production of HLOs, achieving better or comparable liver cell functionality while minimizing growth factor usage and costs.
Article Synopsis
- Primary cultured hepatocytes are essential for studying liver metabolism and toxicity, but they often lose function after isolation due to an unnatural environment.
- Polydimethylsiloxane (PDMS) enhances hepatocyte function due to its high oxygen permeability, but its tendency to absorb chemicals has limited its application in drug development.
- A new material, 4-polymethyl-1-pentene polymer (PMP), maintains liver function effectively for at least a week and shows promise as a superior alternative to PDMS and traditional polystyrene for drug testing.
Article Synopsis
- * A ring-shaped culture vessel was found to produce smaller, more uniform hiPSC aggregates compared to other suspension culture systems, enhancing mass transfer for essential biochemical exchanges.
- * Different culture systems led to varying differentiation tendencies of the aggregates, with smaller ones favoring ectodermal and mesodermal lineages, while larger aggregates leaned towards endodermal lineage, indicating the influence of aggregate size on stem cell differentiation.
Suspension culture is an important method used in the industrial preparation of pluripotent stem cells (PSCs), for regenerative therapy and drug screening. Generally, a suspension culture requires agitation to keep PSC aggregates suspended and to promote mass transfer, but agitation also causes cell damage. In this study, we investigated the use of a Bingham plastic fluid, supplemented with a polysaccharide-based polymer, to preserve PSCs from cell damage in suspension culture.
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