Effect of glypican-1 gene on the pulp cells during the reparative dentine process.

GPC-1 (glypican-1) is a cell surface heparan sulfate proteoglycan that acts as a co-receptor for heparin-binding growth factors and members of the TGF-β (transforming growth factor beta-1) family. The function of cell-surface proteoglycans in the reparative dentine process has been under investigation. Gpc-1 was detected with similar frequency as tgf-β1 in the cDNA library using mRNA from the odontoblast-like cell-enriched pulp of rat incisors.

Micro-computed tomographic comparison of nickel-titanium rotary versus traditional instruments in C-shaped root canal system.

Introduction: The purpose of this study was to assess the efficacy of instrumentation of C-shaped canals with ProTaper rotary system and traditional instruments by using micro-computed tomography (micro-CT).

Methods: Twenty-four mandibular molars with C-shaped canals were selected in pairs and sorted equally into 2 groups, which were assigned for instrumentation by ProTaper rotary system (ProTaper group) or by K-files and Gates-Glidden burs (Hand Instrument group). Three-dimensional images were constructed by micro-CT.

Effects of mineral trioxide aggregate on the differentiation of rat dental pulp cells.

The effect of mineral trioxide aggregate (MTA) on the odontoblast-like differentiation of pulp cells was evaluated using heat-shock protein 25 (hsp25) as a marker for odontoblast differentiation. The cells were cultured with tooth-colored MTA or calcium hydroxide-containing cement (Dycal). The effects of the materials on the pulp cells were observed using a confocal laser scanning microscope.

Analysis of surface roughness and microleakage of fissure sealants following organic debris removal with Carisolv.

Objective: The objective of the present study was to improve fissure sealing by pre-treatment with Carisolv in order to remove organic debris. The surface morphology and roughness of fissure cavities and the degree of microleakage after Carisolv application were compared with those after bristle brush treatment in vitro.

Methods: Fifty extracted human teeth were used in this study.

Light intensity evaluation of laser-induced fluorescence after caries removal using an experimental caries staining agent.

Objective: The purpose of this study was to evaluate the intensity of fluorescent light reflected from cavity wall dentin, after dentin stained by an experimental caries detecting liquid was removed.

Background Data: The conventional caries detecting liquid tends to penetrate dentin too deeply. On the other hand, DIAGNOdent has gradually gained recognition and established itself as a caries detection device using a laser beam.

Artificial plaque removal with Carisolv system: a clinical approach.

In the present study, removal of artifcial plaque in pits and fissures with the Carisolv system was compared with that of conventional bristle brush methoda in vitro. The results indicate that in the dental clinic, complete plaque removal with the Carisolv is possible, and in addition to acid etching, treated cavity was almost free of debris which might increase sealant retention.

Effects of KTP laser irradiation, diode laser, and LED on tooth bleaching: a comparative study.

Objective: This in vitro study examines the whitening efficacy of a light-emitting diode (LED), a diode laser, and a KTP laser irradiation in dental bleaching by analyzing the change in color achieved from the treatment, the temperature increase induced in the pulpal cavity, as well as enamel microhardness measurement after treatment.

Background Data: Bleaching techniques achieved significant advances with the use of coherent or incoherent radiation sources to activate the bleaching agents.

Methods: A hydrogen peroxide bleaching agent, Hi-Lite, was stimulated with an LED, a 980-nm diode laser at 0.

Effect of a novel Er:YAG laser in caries removal and cavity preparation: a clinical observation.

Objective: We evaluated the applicability of a novel Er:YAG laser under clinical conditions.

Background Data: The Er:YAG laser has been demonstrated to be a safe and effective alternative to the conventional turbine bur, but the relatively low cutting speed prevented the wide application of Er:YAG laser in clinical cavity preparation.

Methods: A Smart 2940 D laser developed by Deka Corporation was used for cavity preparation in 95 teeth of 45 patients.

An assessment following root canal preparation by Er,Cr: YSGG laser irradiation in straight and curved roots, in vitro.

In the present study, the effectiveness of Er,Cr:YSGG laser in straight and curved root canal preparation was compared with that of the conventional canal preparation technique, in vitro. The degree of root curvature of 40 root canals was determined, and then 20 canals were prepared by an Er,Cr:YSGG laser of 2 W by using the crown-down technique, while the other 20 canals were shaped by K-file (control). The achievement degree of root canal preparation and debris score was investigated morphologically.

Basic study of morphological changes and surface roughness of cavities prepared by TEA CO2 laser irradiation.

Objective: The purpose of this study was to investigate the morphological change of dental hard tissue and surface roughness of cavities prepared by transversely excited, atmospheric pressure (TEA) CO2 laser irradiation.

Background Data: It has been reported that dental hard tissues and bone can be removed by a long-pulse of TEA CO2 laser irradiation with minimal thermal damage. However, there are few reports on the surface roughness of lased teeth.

Effect of Er,Cr:YSGG laser irradiation on eruption of rat mandibular incisor after disturbance of the enamel organ in the pulp.

To investigate the efficacy of Er,Cr:YSGG (erbium,chromium:yttrium scandium gallium garnet) laser irradiation in root canal preparation and to evaluate its effect on eruption of rat incisors after disturbance of the enamel organ in the pulp, 20 canals of lower left incisor teeth were prepared by K-files followed by Er,Cr:YSGG laser irradiation, and 20 canals of right incisors were subjected to K-files only (control). At 1 week after irradiation, both sides of incisors erupted at the same level from the gingival margin. Histological findings showed that laser irradiation produced a slightly larger damage in the pulp than that of control.

Ultramorphological and histochemical changes after ER,CR:YSGG laser irradiation and two different irrigation regimes.

The purpose of this study was to evaluate the ultramorphological and chemical changes in Er,Cr:YSGG laser-treated radicular dentin in comparison with two different irrigation regimes. Extracted human premolars were assigned into three experimental groups. Group 1: After each file size, root canals were irrigated with 5.

Caries removal with Carisolv system: criteria evaluation and microleakage test.

In the present study, cavities prepared by Carisolv were verified as being caries-free by two methods; by conventional visual and tactile criteria, and by DIAGNOdent. The results indicate that in the dental clinic, the efficiency of complete carious dentin removal with Carisolv is no longer difficult when a proper clinical guide is used. Furthermore, treatment of dentin surfaces with Carisolv is capable of decreasing marginal microleakage after composite resin restorations.

Isolation and partial sequencing of cDNA clones from rat incisor after Nd:YAG laser irradiation in root canal.

Objective: The purpose of this study was to identify the genes expressed in odontoblast-like cells and related to osteodentin production of pulp cells in vivo during the repair process.

Methods: A pulsed Nd:YAG laser was irradiated in mandibular incisors of rats at 2 W and 20 pulses/s for 5 s. We constructed a unidirectional cDNA library from the non-calcified portion of irradiated incisors, sequenced cDNA clones and classified their sequences by homology search through the GenBank database.

Effects of diode laser irradiation on smear layer removal from root canal walls and apical leakage after obturation.

Objective: The objective of this study was to investigate the rise in temperature in root surfaces during and immediately after diode laser irradiation, to observe morphological changes of root canal wall after irradiation, and to evaluate the apical leakage after irradiation and obturation in vitro.

Background Data: There have been very few reports on root canal treatment by 980-nm wavelength diode laser.

Methods: Sixty-six extracted human single-rooted teeth were instrumented up to size 60 K-file, and then randomly divided into three groups of 22 teeth each.

Morphological study of the Er,Cr:YSGG laser for root canal preparation in mandibular incisors with curved root canals.

Objective: The objectives of this study were to observe morphological changes of root canal walls and to evaluate the capability of Er,Cr:YSGG laser for preparation of curved mandibular incisor root canals in vitro.

Background Data: There are no published reports on the use of Er,Cr:YSGG laser for curved root canal preparation.

Methods: Twenty curved mandibular incisor root canals (10 and 15 degrees) were prepared sequentially by laser irradiation at 20 Hz, 2 Wusing two different sizes of fine fiber tips (200 and 320 microm in diameters).

Pulsed Nd:YAG laser effect on eruption of rat mandibular incisors following disturbance of the enamel organ in the pulp.

We investigated the effects of pulsed neodymium:yttrium-aluminium-garnet (Nd:YAG) laser irradiation time on the eruption of 56 mandibular incisors in 28 rats. Clinically, some incisors erupted and the others did not. The incisors were irradiated at 2 W, 20 pulses/s for the period of 3 s, 5 s, 7 s, and 10 s, and the regeneration process was monitored at 20 days after laser treatment.

Efficacy of root canal preparation by Er,Cr:YSGG laser irradiation with crown-down technique in vitro.

Objective: The purpose of this study was to compare the efficacy of Er,Cr:YSGG laser in root canal preparation and its effectiveness in removing debris and smear layer with the efficacy of the conventional hand instrument, in vitro.

Background Data: Recently, the use of the Er,Cr:YSGG laser device in root canal preparation has been excepted in the dental clinic. However, there have been no published reports on root canal preparation by using the Er,Cr:YSGG laser irradiation.

In vivo study of the healing processes that occur in the jaws of rabbits following perforation by an Er,Cr:YSGG laser.

This study investigates the healing process that takes place in the bone and soft tissue of the maxilla and the mandible after perforation by an Er,Cr:YSGG laser device. The jaws of New Zealand white rabbits were irradiated with an Er,Cr:YSGG laser, forming wounds 0.4 mm in diameter.

Morphological comparative study on ablation of dental hard tissues at cavity preparation by Er:YAG and Er,Cr:YSGG lasers.

Objective: The purpose of this study was to evaluate morphologically the dental hard tissue ablation at the class V cavity preparation by two types of laser devices: Er:YAG and Er,Cr:YSGG lasers in vitro.

Background Data: There have been no reports on the comparative study of dental tissue ablation at cavity preparation by Er:YAG and Er,Cr:YSGG lasers.

Methods: Twenty-two extracted human mature molar teeth were used in this study and divided into two groups of 11 teeth each.

Healing process of skin after CO2 laser ablation at low irradiance: a comparison of continuous-wave and pulsed mode.

Objective: The purpose of the present study was to compare the healing process of rabbit's skin after the ablation of continuous wave CO(2) laser and pulsed CO(2) laser at low irradiances.

Background Data: Because of its advantages, the CO(2) laser has been viewed as an alternative to the traditional scalpel. Simple thermal models suggest that suitably short pulses of laser irradiation can leave a smaller thermal damaged zone to ablated tissue, which may lead to faster healing.

Effects on periradicular periodontal tissues of root canal irradiation with Er:YAG laser in rats.

Objective: The effects of Er:YAG laser irradiation on the periodontal region during root canal preparation were evaluated using rats histopathologically.

Background Data: The effects on periodontal tissues along the root surface and apical area during root canal preparation using Er:YAG laser irradiation in vivo are not clear.

Methods: One hundred mesial root canals of mandibular first molars in rats were divided into four groups.

Thermographical and morphological studies of Er,Cr:YSGG laser irradiation on root canal walls.

Objective: This study was performed to investigate the temperature rises on root surfaces and morphological changes on root canal walls of extracted human teeth after irradiation by erbium, chromium: yttrium, scandium, gallium, garnet (Er, Cr:YSGG) laser and to evaluate the efficiency in removing smear layer and debris from the prepared root canal walls in vitro.

Background Data: No report on Er, Cr:YSGG laser effects under various conditions and specific tips for endodontic treatments has been reported.

Methods: Sixty extracted human teeth with single and straight root were used in this study.

Histopathological changes in dental pulp irradiated by Er:YAG laser: a preliminary report on laser pulpotomy.

Objective: The effects of Er:YAG laser irradiation on the pulp tissue during a pulpotomy procedure were evaluated histopathologically.

Background Data: The effects on pulp tissue during laser pulpotomy using Er:YAG laser irradiation are not clear.

Materials And Methods: Sixty mesial root canals of mandibular first molars in rats were divided into four groups.

Comparative study of carious dentin removal by Er,Cr:YSGG laser and Carisolv.

Objective: The present study aimed to compare carious dentin removal by air turbine, Carisolv and erbium,chromium:yttrium,scandium,gallium,garnet (Er,Cr:YSGG) laser, and examine morphological changes before and after these caries removal techniques under light microscopy and scanning electron microscopy (SEM).

Background Data: Although there have been numerous studies on removing caries by Er,Cr:YSGG laser, none has compared Er,Cr:YSGG laser and Carisolv, or reported on the usage of DIAGNOdent as a diagnostic tool particularly for advanced caries in in vitro experiments.

Materials And Methods: Sixty extracted human teeth diagnosed as advanced caries were divided into three groups based on the treatment received, namely air turbine, Carisolv, and Er,Cr:YSGG laser groups.