Changes in the expression of CD106, osteogenic genes, and transcription factors involved in the osteogenic differentiation of human bone marrow mesenchymal stem cells.

Mesenchymal stem cells (MSCs) are well known to possess multipotential differentiation and are becoming a good tool for clinical research. However, specific markers for their purification and the mechanism of their osteogenic differentiation remain to be elucidated. In the present study, we compared the expression of CD106, and osteogenic differentiation-related proteins and genes in human bone marrow (BM)-derived MSCs, before and after differentiation by FACS, histochemical staining, immunohistochemical staining, RT-PCR, and real-time PCR.

Preparation of human immune effector T cells containing iron-oxide nanoparticles.

Preparation of human immune T cells containing iron-oxide nanoparticles was carried out for the development of magnetically mediated immunotherapy. Peripheral blood lymphocytes (PBLs) after the incubation with magnetite nanoparticles were found to contain measurable ferric ions, which suggested the incorporation of magnetite nanoparticles. Transmission electron microscopic (TEM) study indicated that the incorporation of magnetite nanoparticles was mediated by endocytosis of PBLs.

Large-cell neuroendocrine carcinoma of the skin, with lymphoid stroma.

We report two cases of a large-cell neuroendocrine carcinoma of the skin as a distinct histological category. The patients are a 63-year-old woman with a 7-mm reddish round nodule on her nose for 4 months, and a 95-year-old man with several hemorrhagic nodules on the external ear for 28 months. The first patient had two positive sentinel lymph nodes.

Time courses of progress to the chronic stage of middle cerebral artery occlusion models in rats.

Several kinds of middle cerebral artery occlusion model in rats have been developed. Variable ischemic inductions are attributed to the different contributing factors in ischemic damage formation. In the present study, we examined the differences in ischemic induction attributed to chronic stage.