Evaluating the contribution of fat infiltration in anterior gluteus minimus muscle to walking ability in female with unilateral hip osteoarthritis and candidates for total hip arthroplasty.

Background: The purpose of this study is to investigate the relationship between gait and fat infiltration in anterior and posterior gluteus minimus in the patients with hip osteoarthritis.

Methods: Ninety-one female patients who were diagnosed as the unilateral hip osteoarthritis, classified into Kellgren-Lawrence global scoring system grades 3 or 4, and candidate for total hip arthroplasty were retrospectively reviewed. The horizontally cross-sectional regions of interest for the gluteus medius and anterior and posterior gluteus minimus were manually circumscribed in a single transaxial computed tomography image and muscle density of those regions were obtained.

Some properties of beta-1,2-mannosyltransferases related to the biosynthesis of the acid-labile oligomannosyl side chains in Candida albicans NIH B-792 strain cells.

We detected the beta-1,2-mannosyltransferases (beta-1,2-MTs), which participate in the biosynthesis of oligomannosyl side chains in the mannan acid-labile fraction, in a particulate insoluble fractions prepared from Candida albicans NIH B-792 strain cells grown at 27 degrees C and at 37 degrees C in a yeast extract-added Sabouraud liquid medium (YSLM). The beta-1,2-MT VI-6 prepared from the cells grown at 27 degrees C exhibited the maximum activity at pH 7.0 and at 30 degrees C.

Activity and stability of alpha- and beta-mannosyltransferases in Candida albicans cells cultured at high temperature and at low pH.

We measured the activity and stability of three mannosyltransferases to ascertain the mechanisms of changes in the antigenicity and the mannan structure of Candida albicans cells cultured at high temperature (37 degrees C) under acidic (pH 2.0) conditions in a liquid medium. The alpha-1,6-mannosyltransferase (alpha-1,6-MT) activity of the particulate-insoluble enzyme fractions prepared from C.

Antigenicity of Candida tropicalis strain cells cultured at 27 and 37 degrees C.

All cells of four Candida tropicalis strains IFO 0199 (Ct-0199), IFO 0587 (Ct-0587), IFO 1400 (Ct-1400), and IFO 1647 (Ct-1647), obtained by cultivation at 27 and 37 degrees C for 48 h in yeast extract-added Sabouraud liquid medium, showed the shapes of typical budding yeast and the same agglutination patterns against factor sera 1, 4, 5 and 6 in the commercially available kit 'Candida Check'. The cells of the C. tropicalis IFO 0589 strain display the same properties at 27 degrees C but formed hyphae at 37 degrees C.

Antigenicity of cell wall mannans of Candida albicans NIH A-207 strain cells cultured in galactose-added yeast nitrogen base medium.

The cultivations of the Candida albicans NIH A-207 strain (A-strain) for 5 d at 27 and 37 degrees C in 500 mM galactose-added yeast nitrogen base medium (YNB-Gal) decreased the growth of blastoconidia and the pH in the cultures, with dry weights of 56 and 47% and with pHs of 2.41 and 2.47, compared with the dry weight of 100% and pH of 5.