Open-Bundle Structure as the Unfolding Intermediate of Cytochrome ' Revealed by Small Angle Neutron Scattering.

The dynamic structure changes, including the unfolding, dimerization, and transition from the compact to the open-bundle unfolding intermediate structure of Cyt ', were detected by a small-angle neutron scattering experiment (SANS). The structure of Cyt ' was changed into an unstructured random coil at pD = 1.7 (R = 25 Å for the Cyt ' monomer).