Comparative evaluation of platelet-rich fibrin, platelet-rich fibrin + 50 wt% nanohydroxyapatite, platelet-rich fibrin + 50 wt% dentin chips on odontoblastic differentiation - An study-part 2.

Aim: The purpose of this study was to investigate the effects of platelet-rich fibrin (PRF) modified with bioactive radiopacifiers-nanohydroxyapatite (nHA) and dentin chips (DC) on odontoblastic differentiation in human dental pulp cells (HDPCs).

Subjects And Methods: PRF was modified with 50wt% of nHA (G bone-SHAG31, Surgiwear Company) and 50 wt% of DC. HDPSCs differentiation and mineralization by the groups ([Group A - Control (Dimethyl sulfoxide), Group B - PRF, Group C - PRF + nHA, Group D - PRF + DC]) were assessed.

Comparative evaluation of radiopacity and cytotoxicity of platelet-rich fibrin, platelet-rich fibrin + 50wt% nano-hydroxyapatite, platelet-rich fibrin + 50wt% dentin chips: An study.

Aims: The aim of this study is to impart radiopacity to platelet-rich fibrin (PRF) using two different bioactive agents (nano-hydroxyapatite [nHA] and dentin chips [DC]) and to assess the cell proliferation rate induced by the modified PRF.

Subjects And Materials: PRF was modified with 50wt% of nHA (G bone-SHAG31, Surgiwear Company) and 50wt% of DC. The five samples of each group (Group 1 - dentin disc, Group 2 - PRF, Group 3 - PRF + 50wt% nHA, and Group 4-PRF + 50wt% DC) were digitally radiographed together with 8-mm aluminum stepwedge using DIGORA software (for Windows 2.