Publications by authors named "Kotryna Skardziute"
Article Synopsis
- The Mx-TOP method allows for profiling three key epigenetic layers—chromatin accessibility, DNA modification, and DNA hydroxymethylation—from a single sample using a specialized tagging and sequencing technique.
- Validation tests showed that Mx-TOP is highly sensitive and informative in exploring how chromatin and DNA modifications influence gene transcription.
- The method was used to investigate epigenetic changes during neuronal differentiation, revealing that gene body hydroxymethylation (5hmC) plays a crucial role in maintaining promoter accessibility and resisting global changes in chromatin structure.
Due to an extreme rarity of 5-carboxylcytosine (5caC) in the mammalian genome, investigation of its role brings a considerable challenge. Methods based on bisulfite sequencing have been proposed for genome-wide 5caC analysis. However, bisulfite-based sequencing of scarcely abundant 5caC demands significant experimental and computational resources, increasing sequencing cost.
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