Multianalyte serum analysis using mid-infrared spectroscopy.

This study assesses the potential for using mid-infrared (mid-IR) spectroscopy of dried serum films as the basis for the simultaneous quantitation of eight serum analytes: total protein, albumin, triglycerides, cholesterol, glucose, urea, creatinine and uric acid. Infrared transmission spectra were acquired for 300 serum samples, each analysed independently using accepted reference clinical chemical methods. Quantitation methods were based upon the infrared spectra and reference analyses for 200 specimens, and the models validated using the remaining 100 samples.

Quantitation of protein, creatinine, and urea in urine by near-infrared spectroscopy.

Objectives: To determine the feasibility of near-infrared analysis for quantitating urea, creatinine, and protein in urine. Practical advantages of this method include ease of sample presentation and the absence of reagents or disposables.

Design And Methods: The near-infrared methods were developed by first measuring the spectra of 123 different urine samples and, using independent clinical analyses, determining the protein, creatinine, and urea levels in each.

Arthritis diagnosis based upon the near-infrared spectrum of synovial fluid.

Synovial fluid aspirates have been characterized by measuring their visible/near-infrared spectra (400-2500 nm). The hypothesis tested in this study is that the spectra contain sufficient information to serve as an aid in the diagnosis and/or staging of arthritic disorders. The concentrations of all major constituents are carried implicitly in the spectra, and in this sense this approach is similar in spirit to conventional synovial fluid analysis.