[Development and assessment of a reagent kit for RNA detection of CrimeanCongo hemorrhagic fever virus with using reverse transcription loop-mediated isothermal amplification method.].

This study presents the results of laboratory trials of the reagent kit for the rapid detection of RNA of the Crimean-Congo hemorrhagic fever virus (CCHFV) using loop-mediated isothermal amplification with reverse transcription (RT-LAMP). The developed RT-LAMP reagent kit was used to detect the CCHFV and showed a sensitivity of 103 GE/ml of viral RNA, which is sufficient for detection of the CCHFV in the early stage of human infections. The kit showed high specificity and no cross-reactivity with viral panel from the State collection of viruses of the FBRI SRC VB «Vector» (arboviruses and hemorrhagic fever viruses).

[RETROSPECTIVE ANALYSIS OF BIOLOGICAL AND MOLECULAR-GENETIC PROPERTIES OF STRAINS - CAUSATIVE AGENTS OF CHOLERA - ISOLATED IN UKRAINE IN 1994 - 2011].

Aim: Retrospective analysis of biological and molecular-genetic properties of strains - cau- sative agents of cholera - isolated in the period of epidemics in Ukraine in 1994 - 2011.

Materials And Methods: Phenotypic and molecular-genetic properties of 5 strains of cholera vibrios, biovar El Tor isolated from cholera patients and 4 strains from the environmental samples were studied using traditional bacteriological and genetic methods. Detection of DNA for toxigenicity genes and genes characteristic for El Tor and classic biovar were carried out by PCR method using rea- gent kits <> and <> an experimental test system).

[DETERMINATION OF PHYLOGENETIC RELATIONSHIP OF YERSINIA PESTIS STRAINS FROM NATURAL PLAGUE FOCI OF THE CAUCASUS BY MULTI-LO- CUS VNTR-ANALYSIS].

Aim: Determination of the degree of phylogenetic relationship of Yersinia pestis strains iso- lated from the territories of natural foci of plague from the Caucasus using VNTR-typing by 25 loci (MLVA25).

Materials And Methods: 26 strains of Y pestis from Russian natural foci of the Caucasus were used in the study. 25 loci of tandem repeats in Y pestis genome by Le Fleche scheme were used for execution of multi-locus VNTR-analysis.

[EPIZOOTOLOGIC MONITORING OF NATURAL-FOCAL INFECTIONS IN THE SOUTH OF RUSSIA IN 2015].

Aim: Analyze epizootologic manifestations of natural-focal infections (NFI) in the south of Russia in 2015.

Materials And Methods: Reports provided by administrations of Federal Service of Surveillance for Protection of Consumers Rights and Human Welfare, Centers of Hygiene and Epidemiology, Research Institutes for Plague Control and Station for Plague Control were used. Data processing was carried out using Excel program.

A NEW GENETIC VARIANT OF THE CRIMEAN-CONGO HEMORRHAGIC FEVER VIRUS ISOLATED IN CRIMEA.

This work represents the results of the genetic identification of the Crimean-Congo hemorrhagic fever virus (CCHF virus) strains isolated in the Crimean Federal District in conducting the epidemiological survey of the imported case of CCHF from Crimea in 2015. One sample of the serum from a patient and 61 pools (506 specimens) of ticks collected during the epizootiological survey of 6 administrative districts of the Crimean Federal District were tested using PCR for the presence of the CCHF virus RNA. RNA of the CCHF virus was detected in serum from a patient and 10 samples of ticks.

[EPIDEMIOLOGIC SITUATION BY NATURAL-FOCI INFECTIONS IN THE CRIMEA FEDERAL DISTRICT IN 2014-2015].

Aim: Analysis of epidemic manifestations of natural-foci infections (NFI), clarification of spectrum of their causative agents, determination of epizootic activity of natural foci in the Crimea Federal District (KFD).

Materials And Methods: Epizootologic examination of 10 administrative districts of KDF was carried out. 291 pools (2705 specimens) of ixodes ticks and 283 samples of organs of small mammals were studied by PCRmethod for the presence of DNA/RNA of causative agents of a number of NFI.

[PROCUREMENT OF SANITARY AND EPIDEMIOLOGICAL WELFARE IN THE REGION OF-CITY-RESORT SOCHI FOR DANGEROUS AND NATURAL-FOCI INFECTIOUS DIS- EASES IN 2015].

Aim: Analysis ofresults of epidemiologic monitoring especially dangerous, natural-foci and other infectious diseases, asill as epizootologic activity of natural foci of infection on,the terri- tory, of city-resort Sochi.

Materials And Methods: Laboratory studies of 820. samples by PCR; im- mune- and bacteriologic methods were carried out, among those 344 - clinical material, 12 - water from open bodies and 321 - field material.

[Modern methods application of genotyping of infectious diseases pathogens in the context of operational work of specialized anti-epidemic team during the XXII Olympic Winter Games and XI Paralympic Winter Games].

This paper considers the experience of genotyping and sequencing technologies in laboratories of specialized anti-epidemic team (SAET) during the XXII Olympic Winter Games and XI Paralympic Winter Games of 2014 in Sochi. The work carried out during the pre-Olympic period on performance of readiness by SAET for these studies is analyzed. The results of genotyping strains of pathogens during the Olympic Games are presented.