[Morphological features of placenta and expression of collagen-6, MMP-1 and TIMP-1 in placental membranes of women with premature rupture of membranes].

Morphological and immunohistochemical investigation of the women's afterbirth with premature rupture of membranes (PROM) and well-time ruptures of placental membranes has been carried out. Inflammatory infiltration of membranes and placental deficiency were higher at patients with PROM. The absence of inflammatory changes in placental tissue during growth of anhydrous gap was demonstrated.

[Expression of estrogen and progesterone receptors in the endometrium of fertile and infertile women].

The aim of the research is investigation of the endometrial hormonal status during menstrual cycle infertile and infertile women. 68 endometrial biopsies from 18-45 years old women have been studied. Some changes of ER and PR expression during menstrual cycle both in fertile and infertile women has been founded.

[Neuroendocrinal breast cancer].

The complex clinico-morphological and immunohistochemical investigation of neuroendocrinal breast cancer has been carried out. Among 12 observations 3 histological types of neuroendocrinal breast cancer has been founded: parvicellular, colloid and clear-cell cancers. The questions of classification, therapeutic approach, collective and personal prognosis require the optimization of theirs clinico-morphological research with an immunohistochemical verification.

[Morphological and immunohistochemical characteristic of oral mucosa in patients with inflammatory bowel disease].

The aim of presented study was to investigate the morphological and immunohistochemical characteristics of the oral mucosa in patients with Crohn's disease and ulcerative colitis. The study involved 40 patients aged from 18 to 64 years, among them 30 patients with Crohn's disease and 10 patients with ulcerative colitis. Clinical, endoscopic, morphometric and immunohistochemical study have shown that Crohn's disease is characterized by generalized inflammation in the gastrointestinal tract with signs of immune inflammation in the mouth, whereas in ulcerative colitis the pathologic process is limited to the colon.

[Quantitative immunohistochemistry: optimization of analyzing the expression of signaling molecules in the myometrium].

The paper analyzes in detail various aspects of semiautomatic quantitative analysis of the results of immunohistochemical reaction using the Morphology 5.0 computer program to analyze microscopic images. Various procedures for assessing the extent of expression of nuclear and cytoplastic markers are compared and a rationale is provided to choose the value of the relative area of a microimage.

[Detection of endothelial NO synthase in the placenta and nitric oxide in the serum of pregnant women in the complex evaluation of the efficiency of treatment for gestosis].

The content of nitric oxide (NO) in the blood and that of endothelial NO synthase in different placental structures were studied in 107 women with impaired fetoplacental blood flow. The change in the concentration of these substances was established to reflect the efficiency of using this or that treatment mode of gestosis.

[Morphological and immunohistochemical characteristics of oral mucosa in patients with inflammatory intestinal disorders].

The aim of the work was to study morphological and immunological characteristics of oral cavity in patients with Crohn's disease and ulcerative colitis. The group of 40 patients aged 18-64 yr included 30 with Crohn's disease and 10 with ulcerative colitis. They were examined in clinical, endoscopic, morphometric, and immunohistochemical studies.

[Endocrine secretion in endometrial and breast malignant tumors in elderly women].

Hystochemical and immunohystochemical investigations revealed APUD tumor cells in malignant endometrial and breast tumors, which affect elderly women. Identification of the hormones produced by APUD-cells in carcinomas and neuroendocrine tumors could form the basis for selecting the optimal method of treatment, including selection of pills to correct the impaired patients' hormonal status.

[A role of placental macrophages in gestosis pathogenesis in women of various age].

The main aim of this work was to determinate a role of placental macrophages in aetiology of gestosis and significance of pregnant women age during this pathology. We analyzed expression of CD68 (marker of placental macrophages) in placentas of healthy women and women with gestosis from younger and older age groups. We have found that in case of gestosis the increasing expression of placental macrophages at placentas of women younger than 30 years old takes place, although at placentas of women older than 30 years old expression of macrophages remains at stably high level during normal pregnancy flow and in case of gestosis.

[Effect of pineal gland peptides on morphofunctional structure of the pancreas in ageing].

A study of pineal gland peptides effect on morphology and functions of the pancreas in the model of premature ageing in rats was performed with respect to the need in methods for premature ageing prevention. Structural, morphological and functional alterations in pancreas tissue, suggesting premature ageing of the gland, were identified by methods of immunohistochemistry and electronic microscopy. There was registered a geroprotective effect of the pineal gland peptides on pancreas tissue, manifested in the resistance of the latter to the impact of stress factors entailing premature ageing.

[Methodological approaches to quantitative immunohistochemical evaluation of expression of breast apoptosis and proliferation markers].

Apoptosis and proliferative markers were studied in fibroadenoma and fibrocystic breast lesions. A quantitative immunohistochemical test for these factors using computer system of microscopic images is presented. It provides the advantages for interpretation of immunohistochemical reactions by design of expression standards for different pathologies in the breast.