Depolarizing action of allergens on passive sensitized lymphocytes.

An ability of rat thymus lymphocytes to respond to pollen allergens has been found. The lymphocytes were exposed with plasma of pollinosis patients. The subsequent addition of allergens specific for the patient caused the decrease of lymphocyte plasma membrane potential.

[The effect of the plasma of patients with asthma of physical effort on the energy state of the lymphocytes].

The effect of the plasma obtained before and after graded submaximal exercise was examined in 10 bronchial asthma patients with the syndrome of asthma of physical effort (APE) by means of fluorescent exploration using the cation probe 4-(p-demethylaminostyryl)-1-methylpyridinum and rat thymocytes as a test system. Four normal donors and five bronchial asthma patients without the APE syndrome were used as control. The plasma of patients with the APE syndrome, obtained after physical exercise, brought about a decrease of the number of fluorescent cells.

[Methods of measuring the transmembrane electrical potential in cells].

The methods of intracellular microelectrodes, penetrating ions and potential-sensitive fluorescent probes are considered for their possibility to be used for quantitative estimation of transmembrane electrical potentials (TMP) of small cells (mainly through the example of lymphocytes). The following fluorescent methods are described in detail: separate measurement of two TMP components--potentials on the plasma and mitochondrial membranes of a cell; recording of individual differences of cells according to the TMP value. It is supposed that heterogeneity of cells by the TMP value (in particular, the presence of depolarized cells) may be responsible for errors and divergences of the TMP mean values measured by different methods.

[Ryodipine--a new fluorescent probe in the determination of differences between lymphocytes].

Ryodipine (foridon ), a fluorescent compound possessing hypotensive activity, can be useful as fluorescence probe to distinguish between lymphocyte populations. The heterogeneity of cells revealed by the intensity of ryodipine fluorescence is mainly due to differences in the amount of membranous material in the cells. The use of ryodipine for lymphocyte identification has certain advantages over MBA, a known fluorescence probe generally employed for this purpose.

[Effect of lymphocytes on the differentiation of in vitro transformed L-line mouse fibroblasts].

Possible participation of immune system cells in the differentiation of non-lymphoid tissues has been examined. Lymphocytes were tested on transformed fibroblasts of L mouse strain. The presence of lymphocytes in L cell culture enhanced their differentiation, assessed by morphological, proliferative and biophysical criteria.

[Differences between lymphocytes revealed by membrane fluorescent probes].

Interaction of fluorescent probes of 3-methoxybenzanthrone (MBA), 4-dimethylaminochalcone (DMC), and of 1-anilinonaphthalene-8-sulfonate (ANS) with suspensions of rat thymocytes and Peyer's patches lymphocytes has been investigated. By means of the MBA, shown earlier to be a maker of B- and T-lymphocytes, it was established that thymus lymphocytes populations consisted mainly of T-cells (95 +/- 3%), and those of Peyer's patch lymphocytes of B-cells (68 +/- 9%). Definition of parameters of binding and fluorescence of ANS and DMC has shown different physical structure of the thymus and Peyer's patch lymphocyte membranes, resp.