Synthesis of Ni-functionalized polydopamine magnetic beads for facilitated purification of histidine-tagged proteins.

Facilitated purification of proteins, at a low cost and a short time, is one of the key steps in the industrial production of recombinant proteins. In the current study, polydopamine nanoparticles (PDA-NPs) are considered in the synthesis of magnetic beads for purifying recombinant proteins due to advantages such as biocompatibility/ biodegradability, easy synthesis, as well as the ability to directly chelate metal ions. They were synthesized in Tris buffer (pH: 8:5), then chelated with Fe(20 mg) and Ni ions at concentrations of 2, 3, 5, and 7 mg/ml.