Preparation of mixed keratinocyte and melanocyte cultures from biopsy specimens of pigmented skin sites of patients with vitiligo.

A method for preparation and expansion of mixed keratinocyte and melanocyte culture from human skin biopsy specimens was developed. The culture contains two melanocyte types: derivatives of hair follicle stem cells and epidermal basal layer stem cells. Fibroblasts were completely eliminated after culturing in selective medium.

[The therapeutic efficacy of tigazon in treating dermatoses].

Forty patients with various dermatoses (psoriasis, ichthyosis, Darier's disease, discoid lupus erythematosis) were followed-up over the course of treatment with tigason, an aromatic retinoid. The effect was satisfactory in the majority of patients. Side effects were recorded in 16 cases.

[First experience of using autotransfusions of UV-irradiated oxygenated blood in patients with various allergic dermatoses].

This treatment modality has been used in the management of 47 patients with toxicoderma and eczema. No side effects or complications have been observed. The eauthors analyze the literature data and their own findings concerning the mechanisms of action of such autotransfusions.