Human papillomavirus types 16 E1 mRNA is transcribed from P14 early promoter in cervical neoplasms.

High-risk human papillomavirus (HR-HPV) persistent infection is responsible for the development of the majority of cervical cancers. The therapy against HPV-associated cancer requires knowledge of the viral gene expression mechanisms. In this study, the polyadenylated polycistronic transcripts containing full-size E1ORF and produced from the early P14 promoter were detected for the first time in cervical tumors with episomal forms of the HPV16 genome.

[Cloning of alternative isoforms of the catalytic subunit of the human thelomerase (htert)].

Most human tumors, including cervical cancer, are characterized by telomerase activation (cell proliferation activation enzyme). Such activation is implemented in the elongation of the terminal segments (telomeres) of the telomerase chromosome. The gene of the enzyme is RNA-encoded, the RNA in tumors being observed in a few isoforms.

[The clinical morphological concept of origin and course of severe carcinoma in citu (CIN)].

The study was directed to determine objective causes of hypo-diagnostics of neoplastic process of cervix uteri with further development of clinical morphological concept close to understanding and comprehension by practical physicians to avoid diagnostic and tactical errors. The analysis was made concerning diagnostic errors and under-evaluation of degree of neoplasia in 880 patients with CIN of different degree and micro-invasive cancer of cervix uteri. The study also included 187 females with abnormal smears without CIN and cervix uteri cancer.

[The role of cervical transformational zone as an object of human papilloma virus oncogenic effect in cervical intraepithelial neoplasms and invasive cancer development].

The correlation of morphological mistakes in neoplasia grade verification from visibility of transformation zone (TZ) and patient age was studied in 503 patients with CIN and microinvasive cervical cancer. The square of ectocervix lesion was defined by LeiseCap software in colposcopic working station Leisegang 3MV. The exclusive significance of TZ in HPV-associated cancerogenesis was confirmed clinically.

[pAkt expression in cervical intraepithelial neoplasia (CIN) and in microinvasive cervical cancer].

The study objective was an immunohistochemical evaluation of pAkt expression in 81 CIN and microinvasive cervical cancer tissue samples and 10 samples of relatively "normal" cervical epithelium of HPV-infected women. PAkt expression showed significant up-regulation in CIN2, CIN3 and microinvasive cancer in compare to CIN1 and "normal" epithelium. The rate of pAkt- positive cells increased progressively by cervical neoplasia grade advancement reaching 7 +/- 5% in CIN2, 15 +/- 13% in CIN3 and 17 +/- 15% in microinvasive cancer.

[Value of HPV (hybrid capture 2) testing for assessing the effectiveness of conservative surgical treatment in CIN2-3/carcinoma in situ and microinvasive cervical cancer].

The prospective study in 525 patients with CIN2-3/CIS and microinvasive cervical cancer showed the association of epithelial lesions with HPV HR in almost all cases. This allowed using HPV-testing for conservative surgical treatment (ablation, LEEP/ LLETZ, conization) efficacy assessment and patient follow-up. The value of HPV-testing for residual disease and recurrence detection was higher that of cytology and colposcopy.

[Ki-67 expression, thymidine phosphorylase and PTEN in intraepithelial cervical carcinoma].

Expression of Ki-67, thymidine phosphorylase (TP) and PTEN were assessed in various grades of cervical intraepithelial neoplasia (CIN) in order to evaluate their potentials of predicting the gravity of possible damage to the epithelium as well as pro- or regression of CIN. Ki-67 and TP levels were shown to correlate directly with CIN grade. It was suggested that a small number of cases of Ki-67 and TP expression absence (15%), exclusively in CIN3 samples, be due to imminent progression to invasive cancer.

[Are human papillomaviruses responsible for the occurrence of bladder cancer].

A female patient with recurrent bladder cancer underwent complex examination. The primary tumor removed in 2004 showed human papillomavirus (HPV) 16 DNA, mRNA corresponding to HPV16 oncogene E7, as well as HPV16 protein E7. The patient is a smoker who has been working at a chemical factory for over 20 years.

Cervical intraepithelial neoplasia: Telomerase activity and splice pattern of hTERT mRNA.

Cervical cancers are characterized by the persistence of human papilloma virus (HPV) genome that is found in tissue samples starting from the early stages of tumor progression. Just like in other tumors, the activation of telomerase was observed in cervical carcinomas, but information about its expression was controversial. The aim of this study is to find possible correlations between the presence of HPV sequences, activity of telomerase and expression of different spliced forms of hTERT RNA in cervical intraepithelial neoplasias (CIN).

[Clear cell adenocarcinoma of the corpus uteri].

Specimens obtained from 15 patients suffering from a rare form of carcinoma of corpus uteri--a clear cell adenocarcinoma--were studied. The importance of identification of this form of cancer as a specific nosologic unit was shown, since the 5-year survival rate in patients with this lesion (57.2 +/- 16.

[Clinico-morphological characteristics of light-cell adenocarcinoma of the ovaries].

Clinical and morphological data on 37 observations of a rare neoplasm, clear-cell adenocarcinoma of the ovaries (CAO), are presented. Studies of the microstructure permitted the authors to distinguish 4 histological variants: tubular, papillary, solid, and mixed. Comparison of the data on the three-year survival rate with different histological variants of the tumor in all stages and within stage I revealed a higher survival rate in patients with the tubular variant of CAO which is considered to be more favourable prognostically.