Brown fat ATP-citrate lyase links carbohydrate availability to thermogenesis and guards against metabolic stress.

Brown adipose tissue (BAT) engages futile fatty acid synthesis-oxidation cycling, the purpose of which has remained elusive. Here, we show that ATP-citrate lyase (ACLY), which generates acetyl-CoA for fatty acid synthesis, promotes thermogenesis by mitigating metabolic stress. Without ACLY, BAT overloads the tricarboxylic acid cycle, activates the integrated stress response (ISR) and suppresses thermogenesis.

Quantitative analysis of metabolic fluxes in brown fat and skeletal muscle during thermogenesis.

Adaptive thermogenesis by brown adipose tissue (BAT) dissipates calories as heat, making it an attractive anti-obesity target. Yet how BAT contributes to circulating metabolite exchange remains unclear. Here, we quantified metabolite exchange in BAT and skeletal muscle by arteriovenous metabolomics during cold exposure in fed male mice.

Effect of Nanodiamond Sizes on the Efficiency of the Quasi-Specular Reflection of Cold Neutrons.

Nanomaterials can intensively scatter and/or reflect radiation. Such processes and materials are of theoretical and practical interest. Here, we study the quasi-specular reflections (QSRs) of cold neutrons (CNs) and the reflections of very cold neutrons (VCNs) from nanodiamond (ND) powders.

Enhanced directional extraction of very cold neutrons using a diamond nanoparticle powder reflector.

For more than a decade, detonation nanodiamond (DND) powders have been actively studied as a material for efficient reflectors of very cold neutrons (VCNs) and cold neutrons. In this work, we experimentally demonstrate, for the first time, the possibility of enhanced directional extraction of a VCN beam using a reflector made of fluorinated DND powder. With respect to the theoretical flux calculated from an isotropic source at the bottom of the reflector cavity, the gain in the VCN flux density along the beam axis is ∼10 for the neutron velocities of ∼57 and ∼75 m/s.

Proximity labeling of endogenous RICTOR identifies mTOR complex 2 regulation by ADP ribosylation factor ARF1.

Mechanistic target of rapamycin (mTOR) complex 2 (mTORC2) regulates metabolism, cell proliferation, and cell survival. mTORC2 activity is stimulated by growth factors, and it phosphorylates the hydrophobic motif site of the AGC kinases AKT, SGK, and PKC. However, the proteins that interact with mTORC2 to control its activity and localization remain poorly defined.

Effect of Particle Sizes on the Efficiency of Fluorinated Nanodiamond Neutron Reflectors.

Over a decade ago, it was confirmed that detonation nanodiamond (DND) powders reflect very cold neutrons (VCNs) diffusively at any incidence angle and that they reflect cold neutrons quasi-specularly at small incidence angles. In the present publication, we report the results of a study on the effect of particle sizes on the overall efficiency of neutron reflectors made of DNDs. To perform this study, we separated, by centrifugation, the fraction of finer DND nanoparticles (which are referred to as S-DNDs here) from a broad initial size distribution and experimentally and theoretically compared the performance of such a neutron reflector with that from deagglomerated fluorinated DNDs (DF-DNDs).

Clustering of Diamond Nanoparticles, Fluorination and Efficiency of Slow Neutron Reflectors.

Neutrons can be an instrument or an object in many fields of research. Major efforts all over the world are devoted to improving the intensity of neutron sources and the efficiency of neutron delivery for experimental installations. In this context, neutron reflectors play a key role because they allow significant improvement of both economy and efficiency.

Reprogramming to recover youthful epigenetic information and restore vision.

Ageing is a degenerative process that leads to tissue dysfunction and death. A proposed cause of ageing is the accumulation of epigenetic noise that disrupts gene expression patterns, leading to decreases in tissue function and regenerative capacity. Changes to DNA methylation patterns over time form the basis of ageing clocks, but whether older individuals retain the information needed to restore these patterns-and, if so, whether this could improve tissue function-is not known.

Reciprocal Dysregulation of MiR-146b and MiR-451 Contributes in Malignant Phenotype of Follicular Thyroid Tumor.

Over the last few years, incidental thyroid nodules are being diagnosed with increasing frequency with the use of highly sensitive imaging techniques. The ultrasound thyroid gland examination, followed by the fine-needle aspiration cytology is the standard diagnostic approach. However, in cases of the follicular nature of nodules, cytological diagnosis is not enough.

[Comparative analysis of RT-qPCR based methodologies for microRNA detection.].

Many pathological states are accompanied by characteristic changes in the cellular profile of microRNAs - small molecules that regulate gene expression at the posttranscriptional level. This allows us to consider miRNA as a promising class of biological markers. In the work, a direct comparison of three RT-qPCR methodologies (s-Loop, u-Elong and 2-Tail) for miRNA analysis was performed.

Microglia inhibit photoreceptor cell death and regulate immune cell infiltration in response to retinal detachment.

Retinal detachment (RD) is a sight-threatening complication common in many highly prevalent retinal disorders. RD rapidly leads to photoreceptor cell death beginning within 12 h following detachment. In patients with sustained RD, progressive visual decline due to photoreceptor cell death is common, leading to significant and permanent loss of vision.

[Isolation of extracellular micro-vesicles from cell culture medium: comparative evaluation of methods].

Extracellular vesicles (EV) are secreted by cells of multicellular organisms. EV mediate specific mode of intercellular communication by "horizontal" exchange of substances and information. This phenomenon seems to have an essential biological significance and became a subject of intensive research.

Invited article: development of high-field superconducting Ioffe magnetic traps.

We describe the design, construction, and performance of three generations of superconducting Ioffe magnetic traps. The first two are low current traps, built from four racetrack shaped quadrupole coils and two solenoid assemblies. Coils are wet wound with multifilament NbTi superconducting wires embedded in epoxy matrices.

Determination of the Neutron Lifetime Using Magnetically Trapped Neutrons.

We report progress on an experiment to measure the neutron lifetime using magnetically trapped neutrons. Neutrons are loaded into a 1.1 T deep superconducting Ioffe-type trap by scattering 0.

[New trends in the radiodiagnosis of extensive malignancies of the maxilla and nasal cavity and paranasal sinus mucosa].

Application of computed (CT) and magnetic resonance tomography (MRT) provided means of precise sitting of tumor and identifying boundaries of adjacent tissue involvement. The study established certain characteristics of CT detection of bone damage. It also showed MRT superiority in visualizing the soft-tissue component of neoplasm and distinguishing between tumor tissue and concomitant secondary inflammation.

[Computerized tomography of solitary lung metastasis].

Experience has pointed to a rather low potential of traditional procedures of roentgenography and tomography in detecting small foci (ca. 4-5 cm in dia.).

[Computed tomography and magnetic resonance imaging in the diagnosis of accessory nasal sinuses, upper jaw and nasal cavity tumors].

Radiation studies, including computed tomography (CT) and magnetic resonance imaging (MRI), revealed that these techniques can accurately determine the site of a tumor, the borders of its spread to the adjacent anatomic structures. They also revealed the features of CT in detecting osseous structural destruction and the advantage of MRI in visualizing the soft tissue component of a neoplasm and in distinguishing the degree of contrast of tumor tissue and concurrent secondary inflammation. The accuracy of CT and MRI for small tumors is 45-80 and 29% higher than that of X-ray study and traditional tomography, respectively.

[Complex radiation diagnosis of solitary metastases in the lung].

Analyzing about 1000 cases of metastatic lesion of the lung indicated that the incidence of metastases was 12.9% (117 patients). Computed tomography (CT) enhances the informative value of X-ray studies in solitary metastases.

[Analysis of the effect of volley stimulation of the vago-sympathetic trunk on establishment of the heart rate].

In electrical stimulation of the frog vago-sympathetic nerve, oscillations of the cardiac rhythm depended upon changes of the pacemaker MPs.