[Cognitive impairment and social functioning in organic personality disorder due to epilepsy].

Objective: To develop criteria for the diagnosis of cognitive impairment (CI) and to determine the degree of social functioning in organic personality disorder due to epilepsy.

Material And Methods: One hundred and twenty-six patients with epilepsy, aged 18 to 65 years, with disease duration of 11 to 20 years, were examined. The main research method is clinical-psychopathological.

[Dysphoric disorders in epilepsy].

Objective: to determine the differentiated clinical variants of dysphoric disorders in epilepsy based on their syndromal patterns, etiopathogenetic and dynamic characteristics.

Material And Methods: Eighty men (mean age 32.7 years, illness duration from 11 to 20 years) were examined using clinical/psychopathological methods.

[Negative mental disorders in epilepsy].

Objective. To search for clinical features of negative mental disorders in epilepsy. Material and methods.

[Chronic schizophrenia-like psychoses in epilepsy].

Clinical peculiarities of interictal chronic epileptic psychoses were studied in 58 patients. The authors suggested a systematic of schizophrenia-like psychoses in epilepsy. Hallucinoses, paranoid, depressive, manic and catatonic psychotic states were described.

[The clinical and morphological features of the course of tuberculosis in HIV infection].

The development of an acutely progressing process of varying extent to the point of total damage to both lungs is typical of a patient with tuberculosis concurrent with HIV infection due to progressive immunodeficiency. There is an apparent need for dividing patients with comorbidity into 2 groups: (1) HIV/TB, in patients HIV infection is a primary disease; (2) TB/HIV, in whom tuberculosis is accordingly primary. These groups differ in clinical manifestations, forms of tuberculosis, and pathomorphological changes.

DNA structural transitions induced by divalent metal ions in aqueous solutions.

Using methods of IR spectroscopy, light scattering, gel-electrophoresis DNA structural transitions are studied under the action of Cu2+, Zn2+, Mn2+, Ca2+ and Mg2+ ions in aqueous solution. Cu2+, Zn2+, Mn2+ and Ca2+ ions bind both to DNA phosphate groups and bases while Mg2+ ions-only to phosphate groups of DNA. Upon interaction with divalent metal ions studied (except for Mg2+ ions) DNA undergoes structural transition into a compact form.

Complexes of (dG-dC)20 with Mn2+ ions: a study by vibrational circular dichroism and infrared absorption spectroscopy.

The B-Z transition of the synthetic oligonucleotide, (dG-dC)20, induced by Mn2+ ions at room temperature, was investigated by absorption and Vibrational Circular Dichroism (VCD) spectroscopy in the range of 1800-800 cm(-1). Metal ion concentration was varied from 0 to 0.73 M Mn2+ (0 to 8.

DNA interaction with biologically active metal ions. Cooperativity of metal ion binding at compacting of DNA.

The interaction of Cu2+, Mn2+ and Ca2+ ions with DNA in aqueous and water-ethanol solutions at different metal ion concentrations was studied by IR-spectroscopy. At sufficiently high DNA concentrations, DNA interaction with Cu2+, Ca2+ and Mn2+ ions results in compacting of DNA in the aqueous solutions. This process shows a very high cooperativity.

[Calorimetric study of the helix-coil transition in DNA during interaction with Cu2+ ions].

Using the method of differential scanning calorimetry, the DNA helix-coil transition was studied in solutions (10(-3) M Na+, 10(-3) M tris HCl, pH 7.0) containing divalent copper ions at relative metal ion concentrations (Mt2+/PDNA) ranging from 0.2 to 20.

[Calorimetric study of Ca2+ and Mn2+ ions' effect on DNA helix-coil transition].

Using the method of differential scanning calorimetry, the DNA helix-coil transition studied in solutions (10(-3) M Na+, 10(-3) M tris HCl, pH 7.0) containing divalent metal ions (Mn2+ and Ca2+) at relative metal ion concentrations (Me/PDNA) ranging from 0.2 to 20.

[Effect of low temperatures on molecular parameters of DNA].

Cryoconservation effects on macromolecular parameters of DNA from reproductive cells of fishes are studied as well as cryodamages of DNA in solutions under various freezing conditions, by the viscometry method. A dependence of the low temperature action on DNA concentration in solution and on the action duration is determined. It is stated as well that the nitrogen temperature action on the biopolymer solution results in the formation of both double- and single-stranded breaks.

[Interaction of DNA with calcium ions by a vibrational spectroscopy].

DNA complexes with Ca2+ ions formed in films at various relative humidities were studied by IR-spectroscopy for molar concentration ratio of calcium ions and DNA phosphate groups [Ca2+]/[P] = 0.4-20. It is shown that the transition of DNA complexed with Ca2+ ions into B-form occurs at higher average numbers (n) of water molecules absorbed per nucleotide: n > 18 at the ratio [Ca2+]/[P] = 10, n > 24 at [Ca2+]/[P] = 20 and n > 12 in the absence of Ca2+ ions.

[Structural and physicochemical characteristics of DNA from animal tissue subjected to chronic irradiation in the Chernobyl area].

The properties of animal DNAs exposed to prolonged irradiation in the Chernobyl zone, have been studied by the methods of viscometry, thermal denaturation, IR-spectroscopy, and electrophoresis. High content of low-molecular fractions have been observed in the preparations of DNA from liver and spleen, their quantities increasing with age and generation of animals. This effect is especially strong in DNA from liver.

[Study of the interaction of DNA with copper ions using IR- and Raman spectroscopy].

DNA with Cu2+ ions in solution and films is studied by IR and Raman spectroscopy at different relative humidities (R.H. = 51 divided by 98%).

[Study of the interaction of DNA with manganese ions by IR-spectroscopy].

DNA complexed with Mn2+ ions in films is studied at different relative humidities and ion contents ([Mn2+]/[P] = 0.4-1) by IR spectroscopy. It is shown that substantially more sorbed water molecules are necessary for the transition of DNA complexed with Mn2+ ions into the double helical conformation (preferentially B-form) that for macromolecules in the absence of ions.

[Interaction of DNA with divalent metal ions].

The interaction between the native DNA macromolecules and Ca2+, Mn2+, Cu2+ ions in solutions of low ionic strength (10(-3) M Na+) is studied using the methods of differential UV spectroscopy and CD spectroscopy. It is shown that the transition metal ions Mn2+ exercise binding to the nitrogen bases of DNA at concentrations approximately 5 x 10(-6) M and form chelates with guanine of N7-Me(2+)-O6 type. Only at high concentrations in solution (5 x 10(-3) M) do Ca2+ ions interact with the nitrogen bases of native DNA.

Studies of formation of bivalent copper complexes with native and denatured DNA.

The formation of Cu2+ complexes with native and denatured DNA is studied by the methods of differential UV spectroscopy, CD spectroscopy, and viscometry. On ion binding to the bases of native DNA the latter transforms into a new conformation. This transition is accompanied with a sharp increase in UV absorption and a decrease in the intrinsic viscosity though the high degree of helicity persists.

[Changing characteristic viscosity of DNA interacting with Cu2+ and Mn2+ ions].

The interaction of DNA with Cu2+ and Mn2+ ions in low ionic strength solutions (10(-3) M NaCl) has been studied by the viscosimetry method. The DNA characteristic viscosity [eta] decreases sharply at low ion concentrations (addition of 10(-5) M Cu2+ leads to [eta] reduction by a factor of 1.4) and attains certain saturation at high concentrations, the saturation magnitude slightly depending on the ion type.