[Intraosseous blockades and local injection of HYALREPAIR-02 Chondroreparant combination in low back pain treatment].

Objective: Studying the effectiveness of the combined use of intraosseous blockades (IOB) and intramuscular local injection therapy using bottled forms of HYALREPAIR-02 Chondroreparant 02/10 in patients with lumbar dorsopathy and recurrent back pain syndrome.

Material And Methods: 30 patients (16 men and 14 women) with chronic recurrent lumbar dorsopathy with pain and myofascial syndromes were examined. Group 1 included 17 patients, whose treatment included the use of IOB and intramuscular local injection therapy using bottled forms of HYALREPAIR-02 Chondroreparant 02/10.

Pulmonary Sarcoidosis: Experimental Models and Perspectives of Molecular Diagnostics Using Quantum Dots.

Sarcoidosis is a complex inflammatory multisystem disease of unknown etiology that is characterised by epithelioid cell granulomatous lesions affecting various organs, mainly the lungs. In general, sarcoidosis is asymptomatic, but some cases result in severe complications and organ failure. So far, no accurate and validated modelling for clinical and pathohistological manifestations of sarcoidosis is suggested.

Cardiometabolic risk factors and mercury content in hair of women from a territory distant from mercury-rich geochemical zones (Cherepovets city, Northwest Russia).

For the first time in the Russian Federation, a study was conducted to determine the level of mercury in the hair of young and middle-aged women with or without metabolic syndrome (MS) and the Association of mercury content in hair with cardiometabolic risk factors. The studied cohort consisted of 387 women (204 with metabolic syndrome and 183 without metabolic syndrome as controls). The mercury content in the hair was determined by atomic absorption pyrolysis.

Twist-n-Sync: Software Clock Synchronization with Microseconds Accuracy Using MEMS-Gyroscopes.

Sensor networks require a high degree of synchronization in order to produce a stream of data useful for further purposes. Examples of time misalignment manifest as undesired artifacts when doing multi-camera bundle-adjustment or global positioning system (GPS) geo-localization for mapping. Network Time Protocol (NTP) variants of clock synchronization can provide accurate results, though present high variance conditioned by the environment and the channel load.

Development of a fluorescence polarization binding assay for folate receptor.

Folate receptor (FR) has been actively investigated for targeted delivery of therapeutics into cancer cells because this receptor is selectively and highly expressed in carcinomas. Because FR rapidly cycles between the cell surface and cytoplasm, folic acid conjugated to a therapeutic agent can drive targeted therapeutic delivery to cancer cells. We prepared a novel fluorescent ligand Cy5-folate and used it to develop a fluorescence polarization (FP) FR binding assay to determine the binding affinities of FR-targeted molecules.

Development of a fluorescence polarization binding assay for asialoglycoprotein receptor.

Asialoglycoprotein receptor (ASGP-R) has been actively investigated for targeted delivery of therapeutic agents into hepatocytes because this receptor is selectively and highly expressed in liver and has a high internalization rate. Synthetic cluster glycopeptides (e.g.

Development of a microplate fluorescence assay for kynurenine aminotransferase.

Inhibition of kynurenine aminotransferases (KATs) is a strategy to therapeutically reduce levels of kynurenic acid (KYNA), an endogenous antagonist of glutamatergic N-methyl-D-aspartate (NMDA) and cholinergic α₇ nicotinic receptors. Several methods of measuring KAT activity in vitro have been developed, but none is well-suited to high throughput and automation. In this article, we describe a modification of existing high-performance liquid chromatography (HPLC)-based methods that enables the development of a 96-well microplate assay in both enzyme- and cell-based formats using human KAT I as an example.

[Capabilities of telemedicine for solving tasks of psycho-prophylactic work among military servicemen of Air Force command posts].

The article is devoted to using of telecommunicational technologies for prophylaxis, diagnostics and treatment of psychic disorders by military servicemen of specialbuildings of command post of Air Force. Were defined main tasks of using telecomunicational net for improvement of psychic reliability of military operators during a combat duty. Was proposed a scheme of using forces and facilities of medical service in organization of psycho-prophylactic work with using telemedical technologies, was proved necessity of creation of coordination counsel in telemedicine.

Deducing the transmembrane domain organization of presenilin-1 in gamma-secretase by cysteine disulfide cross-linking.

Gamma-secretase is a founding member of membrane-embedded aspartyl proteases that cleave substrates within transmembrane domains, and this enzyme is an important target for the development of therapeutics for Alzheimer's disease. The structure of gamma-secretase and its precise catalytic mechanism still remain largely unknown. Gamma-secretase is a complex of four integral membrane proteins, with presenilin (PS) as the catalytic component.

The initial substrate-binding site of gamma-secretase is located on presenilin near the active site.

gamma-Secretase is a structurally enigmatic multiprotein complex that catalyzes intramembrane proteolysis of a variety of substrates, including the amyloid beta-protein precursor of Alzheimer's disease and the Notch receptor essential to cell differentiation. The active site of this transmembrane aspartyl protease apparently lies at the interface between two subunits of presenilin-1 (PS1); however, evidence suggests the existence of an initial substrate-binding site that is distinct from the active site. Here, we report that photoaffinity probes based on potent helical peptide inhibitors and designed to mimic the amyloid beta-protein precursor substrate bind specifically to the PS subunit interface, at a site close to the active site.

Signal peptide peptidase forms a homodimer that is labeled by an active site-directed gamma-secretase inhibitor.

Presenilin (PS) is the presumptive catalytic component of the intramembrane aspartyl protease gamma-secretase complex. Recently a family of presenilin homologs was identified. One member of this family, signal peptide peptidase (SPP), has been shown to be a protease, which supports the hypothesis that PS and presenilin homologs are related intramembrane-cleaving aspartyl proteases.

Differential effects of inhibitors on the gamma-secretase complex. Mechanistic implications.

Gamma-secretase is a protease complex of four integral membrane proteins, with presenilin (PS) as the apparent catalytic component, and this enzyme processes the transmembrane domains of a variety of substrates, including the amyloid beta-protein precursor and the Notch receptor. Here we explore the mechanisms of structurally diverse gamma-secretase inhibitors by examining their ability to displace an active site-directed photoprobe from PS heterodimers. Most gamma-secretase inhibitors, including a potent inhibitor of the PS-like signal peptide peptidase, blocked the photoprobe from binding to PS1, indicating that these compounds either bind directly to the active site or alter it through an allosteric interaction.

Gamma-secretase/presenilin inhibitors for Alzheimer's disease phenocopy Notch mutations in Drosophila.

Signaling from the Notch (N) receptor is essential for proper cell-fate determinations and tissue patterning in all metazoans. N signaling requires a presenilin (PS)-dependent transmembrane-cleaving activity that is closely related or identical to the gamma-secretase proteolysis of the amyloid-beta precursor protein (APP) involved in Alzheimer's disease pathogenesis. Here, we show that N-[N-(3,5-difluorophenacetyl)-L-alanyl]-(S)-phenylglycine t-butyl ester, a potent gamma-secretase inhibitor reported to reduce amyloid-beta levels in transgenic mice, prevents N processing, translocation, and signaling in cell culture.

Effect of surface charges on the rates of intermolecular electron-transfer between de novo designed metalloproteins.

A de novo designed coiled-coil metalloprotein was prepared that uses electrostatic interactions to control both its conformational and bimolecular electron-transfer properties. The title protein exists as a coiled-coil heterodimer of the [Ru(trpy)(bpy)-KK(37-mer)] and [Ru(NH(3))(5)-EE(37-mer)] polypeptides which is formed by interhelix electrostatic attractions. Circular dichroism studies show that the electrostatic heterodimer has K(d) = 0.

[Molecular mechanisms of autorepair of DNA double strand breaks].

The physical mechanism of interaction between nucleotides in intracellular liquid medium was studied. It was shown that during the contact between the thymine and guanine there exists a potential barrier the distance of about 10 A, which prevents the enzyme self-repairing in DNA after a double damage. All the remaining pairs of nucleotides have no such a barrier.

[Enzyme immunoassay of human chorionic beta-gonadotropin in biological fluids using photographic detection].

Potentialities of a new detection method making use of photographic information transformers were demonstrated as exemplified by enzyme immunoassay of human chorionic beta-gonadotropin. Commercial enzyme immunoassay kits for measurements of human chorionic beta-gonadotropin manufactured by Diaplus joint venture were used in the study. The results of traditional spectrophotometric detection and photographic detection were in good correlation, the correlation coefficient r being equal to 0.

[Biological properties of immunochemically pure tetanus antitoxin].

Immunochemically pure tetanus antitoxin obtained from enzyme-treated horse serum is less reactogenic and anaphylactogenic and possesses higher therapeutic properties than antitoxin purified by nonspecific physico-chemical methods and containing ballast antigens. Due to its increased persistence in the recipient's body, the immunochemically pure antitoxin induces passive immunity in considerably lower doses than the preparations purified by the method "Diaferm-3".